Housekeeping genes for cryopreservation studies on zebrafish embryos and blastomeres
Authors: Lin, C., Spikings, E., Zhang, T. and Rawson, D.
Journal: Theriogenology
Volume: 71
Issue: 7
Pages: 1147-1155
ISSN: 0093-691X
DOI: 10.1016/j.theriogenology.2008.12.013
Abstract:Cryopreservation success is usually analysed in terms of cell survival, although there are other potential effects that do not necessarily result in cell death. These include DNA damage, which could result in altered gene expression. Real-time reverse transcriptase PCR allows quantitative analysis of gene expression but usually requires analysis of a 'housekeeping' gene as an internal reference. As the stability of housekeeping genes varies significantly among different groups of samples, it is recommended that those chosen are validated for each different type of sample group. This study aimed to validate housekeeping genes for use in cryopreservation studies of zebrafish embryos. Seven potential housekeeping genes were analysed across fresh and chilled intact embryos and across fresh and frozen isolated blastomeres using the GeNorm and NormFinder software packages. Results suggest that combined use of β-actin and EF1α as housekeeping genes would be suitable for cryopreservation studies on zebrafish embryos and blastomeres. © 2009 Elsevier Inc. All rights reserved.
Source: Scopus
Housekeeping genes for cryopreservation studies on zebrafish embryos and blastomeres.
Authors: Lin, C., Spikings, E., Zhang, T. and Rawson, D.
Journal: Theriogenology
Volume: 71
Issue: 7
Pages: 1147-1155
ISSN: 0093-691X
DOI: 10.1016/j.theriogenology.2008.12.013
Abstract:Cryopreservation success is usually analysed in terms of cell survival, although there are other potential effects that do not necessarily result in cell death. These include DNA damage, which could result in altered gene expression. Real-time reverse transcriptase PCR allows quantitative analysis of gene expression but usually requires analysis of a 'housekeeping' gene as an internal reference. As the stability of housekeeping genes varies significantly among different groups of samples, it is recommended that those chosen are validated for each different type of sample group. This study aimed to validate housekeeping genes for use in cryopreservation studies of zebrafish embryos. Seven potential housekeeping genes were analysed across fresh and chilled intact embryos and across fresh and frozen isolated blastomeres using the GeNorm and NormFinder software packages. Results suggest that combined use of beta-actin and EF1alpha as housekeeping genes would be suitable for cryopreservation studies on zebrafish embryos and blastomeres.
Source: PubMed
Preferred by: Tiantian Zhang
Housekeeping genes for cryopreservation studies on zebrafish embryos and blastomeres
Authors: Lin, C., Spikings, E., Zhang, T. and Rawson, D.
Journal: THERIOGENOLOGY
Volume: 71
Issue: 7
Pages: 1147-1155
eISSN: 1879-3231
ISSN: 0093-691X
DOI: 10.1016/j.theriogenology.2008.12.013
Source: Web of Science (Lite)
Housekeeping genes for cryopreservation studies on zebrafish embryos and blastomeres.
Authors: Lin, C., Spikings, E., Zhang, T. and Rawson, D.
Journal: Theriogenology
Volume: 71
Issue: 7
Pages: 1147-1155
eISSN: 1879-3231
ISSN: 0093-691X
DOI: 10.1016/j.theriogenology.2008.12.013
Abstract:Cryopreservation success is usually analysed in terms of cell survival, although there are other potential effects that do not necessarily result in cell death. These include DNA damage, which could result in altered gene expression. Real-time reverse transcriptase PCR allows quantitative analysis of gene expression but usually requires analysis of a 'housekeeping' gene as an internal reference. As the stability of housekeeping genes varies significantly among different groups of samples, it is recommended that those chosen are validated for each different type of sample group. This study aimed to validate housekeeping genes for use in cryopreservation studies of zebrafish embryos. Seven potential housekeeping genes were analysed across fresh and chilled intact embryos and across fresh and frozen isolated blastomeres using the GeNorm and NormFinder software packages. Results suggest that combined use of beta-actin and EF1alpha as housekeeping genes would be suitable for cryopreservation studies on zebrafish embryos and blastomeres.
Source: Europe PubMed Central