cpDNA-RFLP in Ceramium (Rhodophyta): Intraspecific polymorphism and species-level phylogeny
Authors: Wattier, R.A., Davidson, A.L., Ward, B.A. and Maggs, C.A.
Journal: American Journal of Botany
Volume: 88
Issue: 7
Pages: 1209-1213
ISSN: 0002-9122
DOI: 10.2307/3558331
Abstract:Restriction fragment length polymorphism (RFLP) analysis of chloroplast (cp) DNA is a powerful tool for the study of microevolutionary processes in land plants, yet has not previously been applied to seaweed populations. We used cpDNA-RFLR detected on Southern blots using labeled total plastid DNA, to search for intraspecific and intrapopulational cpDNA RFLP polymorphism in two species of the common red algal genus Ceramium in Ireland and Britain. In C. botryocarpum one polymorphism was detected in one individual among 18 from two populations. Twenty-six individuals of C. virgatum from five populations at three locations exhibited a total of four haplotypes. One was frequent (80.8% of individuals); the others were rare (7.7, 7.7, and 4.2%) and were private to particular populations. Polymorphism was observed in two populations. The corrected mean was 2.26±0.36 haplotypes per population, which was within the typical range determined for higher plants using similar techniques. The spatial distribution of haplotypes was heterogeneous, with highly significant population differentiation (P = 0.00018; Fisher's exact test). Intraspecific polymorphism in C. virgatum had no impact on species-level phylogenetic reconstruction. This is the first unequivocal report of both intraspecific and intrapopulational cpDNA-RFLP polymorphism in algae.
Source: Scopus
cpDNA-RFLP in Ceramium (Rhodophyta): intraspecific polymorphism and species-level phylogeny.
Authors: Wattier, R.A., Davidson, A.L., Ward, B.A. and Maggs, C.A.
Journal: Am J Bot
Volume: 88
Issue: 7
Pages: 1209-1213
ISSN: 0002-9122
Abstract:Restriction fragment length polymorphism (RFLP) analysis of chloroplast (cp) DNA is a powerful tool for the study of microevolutionary processes in land plants, yet has not previously been applied to seaweed populations. We used cpDNA-RFLP, detected on Southern blots using labeled total plastid DNA, to search for intraspecific and intrapopulational cpDNA RFLP polymorphism in two species of the common red algal genus Ceramium in Ireland and Britain. In C. botryocarpum one polymorphism was detected in one individual among 18 from two populations. Twenty-six individuals of C. virgatum from five populations at three locations exhibited a total of four haplotypes. One was frequent (80.8% of individuals); the others were rare (7.7, 7.7, and 4.2%) and were private to particular populations. Polymorphism was observed in two populations. The corrected mean was 2.26 ± 0.36 haplotypes per population, which was within the typical range determined for higher plants using similar techniques. The spatial distribution of haplotypes was heterogeneous, with highly significant population differentiation (P = 0.00018; Fisher's exact test). Intraspecific polymorphism in C. virgatum had no impact on species-level phylogenetic reconstruction. This is the first unequivocal report of both intraspecific and intrapopulational cpDNA-RFLP polymorphism in algae.
Source: PubMed
cpDNA-RFLP in <i>Ceramium</i> (Rhodophyta):: Intraspecific polymorphism and species-level phylogeny
Authors: Wattier, R.A., Davidson, A.L., Ward, B.A. and Maggs, C.A.
Journal: AMERICAN JOURNAL OF BOTANY
Volume: 88
Issue: 7
Pages: 1209-1213
ISSN: 0002-9122
DOI: 10.2307/3558331
Source: Web of Science (Lite)
cpDNA-RFLP in Ceramium (Rhodophyta): intraspecific polymorphism and species-level phylogeny.
Authors: Wattier, R.A., Davidson, A.L., Ward, B.A. and Maggs, C.A.
Journal: American journal of botany
Volume: 88
Issue: 7
Pages: 1209-1213
eISSN: 1537-2197
ISSN: 1537-2197
DOI: 10.2307/3558331
Abstract:Restriction fragment length polymorphism (RFLP) analysis of chloroplast (cp) DNA is a powerful tool for the study of microevolutionary processes in land plants, yet has not previously been applied to seaweed populations. We used cpDNA-RFLP, detected on Southern blots using labeled total plastid DNA, to search for intraspecific and intrapopulational cpDNA RFLP polymorphism in two species of the common red algal genus Ceramium in Ireland and Britain. In C. botryocarpum one polymorphism was detected in one individual among 18 from two populations. Twenty-six individuals of C. virgatum from five populations at three locations exhibited a total of four haplotypes. One was frequent (80.8% of individuals); the others were rare (7.7, 7.7, and 4.2%) and were private to particular populations. Polymorphism was observed in two populations. The corrected mean was 2.26 ± 0.36 haplotypes per population, which was within the typical range determined for higher plants using similar techniques. The spatial distribution of haplotypes was heterogeneous, with highly significant population differentiation (P = 0.00018; Fisher's exact test). Intraspecific polymorphism in C. virgatum had no impact on species-level phylogenetic reconstruction. This is the first unequivocal report of both intraspecific and intrapopulational cpDNA-RFLP polymorphism in algae.
Source: Europe PubMed Central