Retention reproducibility of basic drugs in high-performance liquid chromatography on a silica column with a methanol-high-pH buffer eluent. Changes in selectivity with the age of the stationary phase
Authors: Smith, R.M., Westlake, J.P., Gill, R. and Osselton, M.D.
Journal: Journal of Chromatography A
Volume: 592
Issue: 1-2
Pages: 85-92
ISSN: 0021-9673
DOI: 10.1016/0021-9673(92)85076-6
Abstract:The long-term reproducibility of the separation of basic drugs by high-performance liquid chromatography using a silica column with a methanol-high-pH eluent was examined. The relative capacity factors of specific tertiary amines changed systematically compared with the other drugs on storage of the columns for 1 year. The changes were enhanced on storing the column in mobile phase and were accelerated by prolonged washing with water. A comparison of freshly packed columns suggested that similar changes also occurred with the dry silica on storage. These effects may be a contributor to previously observed batch-to-batch differences in the silica. © 1992.
Source: Scopus
Retention reproducibility of basic drugs in high-performance liquid chromatography on a silica column with a methanol-high-pH buffer eluent. Changes in selectivity with the age of the stationary phase.
Authors: Smith, R.M., Westlake, J.P., Gill, R. and Osselton, M.D.
Journal: J Chromatogr
Volume: 592
Issue: 1-2
Pages: 85-92
DOI: 10.1016/0021-9673(92)85076-6
Abstract:The long-term reproducibility of the separation of basic drugs by high-performance liquid chromatography using a silica column with a methanol-high-pH eluent was examined. The relative capacity factors of specific tertiary amines changed systematically compared with the other drugs on storage of the columns for 1 year. The changes were enhanced on storing the column in mobile phase and were accelerated by prolonged washing with water. A comparison of freshly packed columns suggested that similar changes also occurred with the dry silica on storage. These effects may be a contributor to previously observed batch-to-batch differences in the silica.
Source: PubMed
Preferred by: David Osselton
Retention reproducibility of basic drugs in high-performance liquid chromatography on a silica column with a methanol-high-pH buffer eluent. Changes in selectivity with the age of the stationary phase.
Authors: Smith, R.M., Westlake, J.P., Gill, R. and Osselton, M.D.
Journal: Journal of chromatography
Volume: 592
Issue: 1-2
Pages: 85-92
DOI: 10.1016/0021-9673(92)85076-6
Abstract:The long-term reproducibility of the separation of basic drugs by high-performance liquid chromatography using a silica column with a methanol-high-pH eluent was examined. The relative capacity factors of specific tertiary amines changed systematically compared with the other drugs on storage of the columns for 1 year. The changes were enhanced on storing the column in mobile phase and were accelerated by prolonged washing with water. A comparison of freshly packed columns suggested that similar changes also occurred with the dry silica on storage. These effects may be a contributor to previously observed batch-to-batch differences in the silica.
Source: Europe PubMed Central