Citreoviridin, a specific inhibitor of the mitochondrial adenosine triphosphatase
Authors: Linnett, P.E., Mitchell, A.D., Osselton, M.D., Mulheirn, L.J. and Beechey, R.B.
Journal: Biochemical Journal
Volume: 170
Issue: 3
Pages: 503-510
ISSN: 0264-6021
DOI: 10.1042/bj1700503
Source: Scopus
Citreoviridin, a specific inhibitor of the mitochondiral adenosine triphosphatase.
Authors: Linnett, P.E., Mitchell, A.D., Osselton, M.D., Mulheirn, L.J. and Beechey, R.B.
Journal: Biochem J
Volume: 170
Issue: 3
Pages: 503-510
ISSN: 0264-6021
DOI: 10.1042/bj1700503
Abstract:1. Citreoviridin was a potent inhibitor of the soluble mitochondrial ATPase (adenosine triphosphatase) similar to the closely related aurovertins B and D. 2. Citreoviridin inhibited the following mitochondrial energy-linked reactions also: ADP-stimulated respiration in whole mitochondria from ox heart and rat liver; ATP-driven reduction of NAD+ by succinate; ATP-driven NAD transhydrogenase and ATPase from ox heart submitochondrial particles. 3. The dissociation constant (KD) calculated by a simple law-of-mass-action treatment for the citreoviridin--ATPase complex was 0.5--4.2micron for ox-heart mitochondrial preparations and 0.15micron for rat liver mitochondria. 4. Monoacetylation of citreoviridin decreased its inhibitory potency (KD=2--25micron, ox heart; KD=0.7micron, rat liver). Diacetylation greatly decreased the inhibitory potency (KD=60--215micron, ox heart). 5. Hydrogenation of citreoviridin monoacetate diminished its inhibitory potency considerably. 6. No significant enhancement of fluorescence was observed when citreoviridin interacted with the mitochondrial ATPase.
Source: PubMed
Preferred by: David Osselton
Citreoviridin, a specific inhibitor of the mitochondiral adenosine triphosphatase.
Authors: Linnett, P.E., Mitchell, A.D., Osselton, M.D., Mulheirn, L.J. and Beechey, R.B.
Journal: The Biochemical journal
Volume: 170
Issue: 3
Pages: 503-510
eISSN: 1470-8728
ISSN: 0264-6021
DOI: 10.1042/bj1700503
Abstract:1. Citreoviridin was a potent inhibitor of the soluble mitochondrial ATPase (adenosine triphosphatase) similar to the closely related aurovertins B and D. 2. Citreoviridin inhibited the following mitochondrial energy-linked reactions also: ADP-stimulated respiration in whole mitochondria from ox heart and rat liver; ATP-driven reduction of NAD+ by succinate; ATP-driven NAD transhydrogenase and ATPase from ox heart submitochondrial particles. 3. The dissociation constant (KD) calculated by a simple law-of-mass-action treatment for the citreoviridin--ATPase complex was 0.5--4.2micron for ox-heart mitochondrial preparations and 0.15micron for rat liver mitochondria. 4. Monoacetylation of citreoviridin decreased its inhibitory potency (KD=2--25micron, ox heart; KD=0.7micron, rat liver). Diacetylation greatly decreased the inhibitory potency (KD=60--215micron, ox heart). 5. Hydrogenation of citreoviridin monoacetate diminished its inhibitory potency considerably. 6. No significant enhancement of fluorescence was observed when citreoviridin interacted with the mitochondrial ATPase.
Source: Europe PubMed Central