The extraction and analysis of benzodiazepines in tissues by enzymic digestion and high‐performance liquid chromatography
Authors: OSSELTON, M.D., HAMMOND, M.D. and TWITCHETT, P.J.
Journal: Journal of Pharmacy and Pharmacology
Volume: 29
Issue: 1
Pages: 460-462
eISSN: 2042-7158
ISSN: 0022-3573
DOI: 10.1111/j.2042-7158.1977.tb11369.x
Abstract:A simple, rapid and sensitive method is described for the analysis of unchanged benzodiazepine drugs and their metabolites, in human and animal tissues, using the proteolytic enzyme subtilisin Carlsberg followed by reverse‐phase liquid chromatography. The enzymic digestion yields far higher recoveries than conventional extraction methods, while the liquid chromatographic analysis allows the rapid separation and detection of nanogram quantities of these drugs without the need for elaborate “clean‐up” procedures. 1977 Royal Pharmaceutical Society of Great Britain
Source: Scopus
The extraction and analysis of benzodiazepines in tissues by enzymic digestion and high-performance liquid chromatography.
Authors: Osselton, M.D., Hammond, M.D. and Twitchett, P.J.
Journal: J Pharm Pharmacol
Volume: 29
Issue: 8
Pages: 460-462
ISSN: 0022-3573
DOI: 10.1111/j.2042-7158.1977.tb11369.x
Abstract:A simple, rapid and sensitive method is described for the analysis of unchanged benzodiazepine drugs and their metabolites, in human and animal tissues, using the proteolytic enzyme subtilisin Carlsberg followed by reverse-phase liquid chromatography. The enzymic digestion yields far higher recoveries than conventional extraction methods, while the liquid chromatogrpahic analysis allows the rapid separation and detection of nanogram quantities of these drugs without the need for elaborate "clean-up" procedures.
Source: PubMed
Preferred by: David Osselton
The extraction and analysis of benzodiazepines in tissues by enzymic digestion and high-performance liquid chromatography.
Authors: Osselton, M.D., Hammond, M.D. and Twitchett, P.J.
Journal: The Journal of pharmacy and pharmacology
Volume: 29
Issue: 8
Pages: 460-462
eISSN: 2042-7158
ISSN: 0022-3573
DOI: 10.1111/j.2042-7158.1977.tb11369.x
Abstract:A simple, rapid and sensitive method is described for the analysis of unchanged benzodiazepine drugs and their metabolites, in human and animal tissues, using the proteolytic enzyme subtilisin Carlsberg followed by reverse-phase liquid chromatography. The enzymic digestion yields far higher recoveries than conventional extraction methods, while the liquid chromatogrpahic analysis allows the rapid separation and detection of nanogram quantities of these drugs without the need for elaborate "clean-up" procedures.
Source: Europe PubMed Central