Retention reproducibility of basic drugs in high-performance liquid chromatography on a silica column with a methanol-ammonium nitrate solvent. Interlaboratory collaborative study

Authors: Gill, R., Osselton, M.D., Smith, R.M. and Hurdley, T.G.

Journal: Journal of Chromatography A

Volume: 386

Issue: C

Pages: 65-77

ISSN: 0021-9673

DOI: 10.1016/S0021-9673(01)94585-6

Abstract:

The reproducibility of the separation of basic drugs on silica columns has been tested in a collaborative study between nine laboratories. The results from the different laboratories were very similar. Various methods of recording the retention properties of the drugs were compared with reference to their reproducibility and ability to discriminate between different compounds. Relative retention times, relative capacity factors, and corrected capacity factors were much better than retention times and capacity factors, although all the methods suffered from large errors with weakly retained compounds. All the work was carried out using a single batch of silica to avoid variations due to the stationary phase. © 1987.

Source: Scopus

Retention reproducibility of basic drugs in high-performance liquid chromatography on a silica column with a methanol-ammonium nitrate eluent. Interlaboratory collaborative study.

Authors: Gill, R., Osselton, M.D., Smith, R.M. and Hurdley, T.G.

Journal: J Chromatogr

Volume: 386

Pages: 65-77

DOI: 10.1016/s0021-9673(01)94585-6

Abstract:

The reproducibility of the separation of basic drugs on silica columns has been tested in a collaborative study between nine laboratories. The results from the different laboratories were very similar. Various methods of recording the retention properties of the drugs were compared with reference to their reproducibility and ability to discriminate between different compounds. Relative retention times, relative capacity factors, and corrected capacity factors were much better than retention times and capacity factors, although all the methods suffered from large errors with weakly retained compounds. All the work was carried out using a single batch of silica to avoid variations due to the stationary phase.

Source: PubMed

Preferred by: David Osselton

Retention reproducibility of basic drugs in high-performance liquid chromatography on a silica column with a methanol-ammonium nitrate eluent. Interlaboratory collaborative study.

Authors: Gill, R., Osselton, M.D., Smith, R.M. and Hurdley, T.G.

Journal: Journal of chromatography

Volume: 386

Pages: 65-77

DOI: 10.1016/s0021-9673(01)94585-6

Abstract:

The reproducibility of the separation of basic drugs on silica columns has been tested in a collaborative study between nine laboratories. The results from the different laboratories were very similar. Various methods of recording the retention properties of the drugs were compared with reference to their reproducibility and ability to discriminate between different compounds. Relative retention times, relative capacity factors, and corrected capacity factors were much better than retention times and capacity factors, although all the methods suffered from large errors with weakly retained compounds. All the work was carried out using a single batch of silica to avoid variations due to the stationary phase.

Source: Europe PubMed Central