The gusBC genes of Escherichia coli encode a glucuronide transport system.
Authors: Liang, W.-J., Wilson, K.J., Xie, H., Knol, J., Suzuki, S., Rutherford, N.G., Henderson, P.J.F. and Jefferson, R.A.
Journal: J Bacteriol
Volume: 187
Issue: 7
Pages: 2377-2385
ISSN: 0021-9193
DOI: 10.1128/JB.187.7.2377-2385.2005
Abstract:Two genes, gusB and gusC, from a natural fecal isolate of Escherichia coli are shown to encode proteins responsible for transport of beta-glucuronides with synthetic [(14)C]phenyl-1-thio-beta-d-glucuronide as the substrate. These genes are located in the gus operon downstream of the gusA gene on the E. coli genome, and their expression is induced by a variety of beta-d-glucuronides. Measurements of transport in right-side-out subcellular vesicles show the system has the characteristics of secondary active transport energized by the respiration-generated proton motive force. When the genes were cloned together downstream of the tac operator-promoter in the plasmid pTTQ18 expression vector, transport activity was increased considerably with isopropylthiogalactopyranoside as the inducer. Amplified expression of the GusB and GusC proteins enabled visualization and identification by N-terminal sequencing of both proteins, which migrated at ca. 32 kDa and 44 kDa, respectively. Separate expression of the GusB protein showed that it is essential for glucuronide transport and is located in the inner membrane, while the GusC protein does not catalyze transport but assists in an as yet unknown manner and is located in the outer membrane. The output of glucuronides as waste by mammals and uptake for nutrition by gut bacteria or reabsorption by the mammalian host is discussed.
Source: PubMed
The gusBC Genes of Escherichia coli Encode a Glucuronide Transport System
Authors: Liang, W.-J., Wilson, K.J., Xie, H., Knol, J., Suzuki, S., Rutherford, N.G., Henderson, P.J. and Jefferson, R.A.
Journal: Journal of Bacteriology
Volume: 187
Pages: 2377-2385
ISSN: 0021-9193
DOI: 10.1128/JB.187.7.2377-2385.2005
Abstract:Two genes, gusB and gusC, from a natural fecal isolate of Escherichia coli are shown to encode proteins responsible for transport of ß-glucuronides with synthetic [14C]phenyl-1-thio-ß-D-glucuronide as the substrate. These genes are located in the gus operon downstream of the gusA gene on the E. coli genome, and their expression is induced by a variety of ß-D-glucuronides. Measurements of transport in right-side-out subcellular vesicles show the system has the characteristics of secondary active transport energized by the respiration-generated proton motive force. When the genes were cloned together downstream of the tac operator-promoter in the plasmid pTTQ18 expression vector, transport activity was increased considerably with isopropylthiogalactopyranoside as the inducer. Amplified expression of the GusB and GusC proteins enabled visualization and identification by N-terminal sequencing of both proteins, which migrated at ca. 32 kDa and 44 kDa, respectively. Separate expression of the GusB protein showed that it is essential for glucuronide transport and is located in the inner membrane, while the GusC protein does not catalyze transport but assists in an as yet unknown manner and is located in the outer membrane. The output of glucuronides as waste by mammals and uptake for nutrition by gut bacteria or reabsorption by the mammalian host is discussed.
http://jb.asm.org/cgi/content/abstract/187/7/2377
Source: Manual
Preferred by: Wei-Jun Liang
The gusBC genes of Escherichia coli encode a glucuronide transport system.
Authors: Liang, W.-J., Wilson, K.J., Xie, H., Knol, J., Suzuki, S., Rutherford, N.G., Henderson, P.J.F. and Jefferson, R.A.
Journal: Journal of bacteriology
Volume: 187
Issue: 7
Pages: 2377-2385
eISSN: 1098-5530
ISSN: 0021-9193
DOI: 10.1128/jb.187.7.2377-2385.2005
Abstract:Two genes, gusB and gusC, from a natural fecal isolate of Escherichia coli are shown to encode proteins responsible for transport of beta-glucuronides with synthetic [(14)C]phenyl-1-thio-beta-d-glucuronide as the substrate. These genes are located in the gus operon downstream of the gusA gene on the E. coli genome, and their expression is induced by a variety of beta-d-glucuronides. Measurements of transport in right-side-out subcellular vesicles show the system has the characteristics of secondary active transport energized by the respiration-generated proton motive force. When the genes were cloned together downstream of the tac operator-promoter in the plasmid pTTQ18 expression vector, transport activity was increased considerably with isopropylthiogalactopyranoside as the inducer. Amplified expression of the GusB and GusC proteins enabled visualization and identification by N-terminal sequencing of both proteins, which migrated at ca. 32 kDa and 44 kDa, respectively. Separate expression of the GusB protein showed that it is essential for glucuronide transport and is located in the inner membrane, while the GusC protein does not catalyze transport but assists in an as yet unknown manner and is located in the outer membrane. The output of glucuronides as waste by mammals and uptake for nutrition by gut bacteria or reabsorption by the mammalian host is discussed.
Source: Europe PubMed Central