Detection of ketamine and its metabolites in urine by ultra high pressure liquid chromatography-tandem mass spectrometry
Authors: Parkin, M.C., Turfus, S.C., Smith, N.W., Halket, J.M., Braithwaite, R.A., Elliott, S.P., Osselton, M.D., Cowan, D.A. and Kicman, A.T.
Journal: Journal of Chromatography B Analytical Technologies in the Biomedical and Life Sciences
Volume: 876
Issue: 1
Pages: 137-142
ISSN: 1570-0232
DOI: 10.1016/j.jchromb.2008.09.036
Abstract:Current analytical methods used for screening drugs and their metabolites in biological samples from victims of drug-facilitated sexual assault (DFSA) or other vulnerable groups can lack sufficient sensitivity. The application of liquid chromatography, employing small particle sizes, with tandem mass spectrometry (MS/MS) is likely to offer the sensitivity required for detecting candidate drugs and/or their metabolites in urine, as demonstrated here for ketamine. Ultra-performance liquid chromatography-mass spectrometry (UPLC-MS/MS) was performed following extraction of urine (4 mL) using mixed-mode (cation and C8) solid-phase cartridges. Only 20 μL of the 250 μL extract was injected, leaving sufficient volume for other assays important in DFSA cases. Three ion transitions were chosen for confirmatory purposes. As ketamine and norketamine (including their stable isotopes) are available as reference standards, the assay was additionally validated for quantification purposes to study elimination of the drug and primary metabolite following a small oral dose of ketamine (50 mg) in 6 volunteers. Dehydronorketamine, a secondary metabolite, was also analyzed qualitatively to determine whether monitoring could improve retrospective detection of administration. The detection limit for ketamine and norketamine was 0.03 ng/mL and 0.05 ng/mL, respectively, and these compounds could be confirmed in urine for up to 5 and 6 days, respectively. Dehydronorketamine was confirmed up to 10 days, providing a very broad window of detection. © 2008.
Source: Scopus
Detection of ketamine and its metabolites in urine by ultra high pressure liquid chromatography-tandem mass spectrometry.
Authors: Parkin, M.C., Turfus, S.C., Smith, N.W., Halket, J.M., Braithwaite, R.A., Elliott, S.P., Osselton, M.D., Cowan, D.A. and Kicman, A.T.
Journal: J Chromatogr B Analyt Technol Biomed Life Sci
Volume: 876
Issue: 1
Pages: 137-142
ISSN: 1570-0232
DOI: 10.1016/j.jchromb.2008.09.036
Abstract:Current analytical methods used for screening drugs and their metabolites in biological samples from victims of drug-facilitated sexual assault (DFSA) or other vulnerable groups can lack sufficient sensitivity. The application of liquid chromatography, employing small particle sizes, with tandem mass spectrometry (MS/MS) is likely to offer the sensitivity required for detecting candidate drugs and/or their metabolites in urine, as demonstrated here for ketamine. Ultra-performance liquid chromatography-mass spectrometry (UPLC-MS/MS) was performed following extraction of urine (4 mL) using mixed-mode (cation and C8) solid-phase cartridges. Only 20 microL of the 250 microL extract was injected, leaving sufficient volume for other assays important in DFSA cases. Three ion transitions were chosen for confirmatory purposes. As ketamine and norketamine (including their stable isotopes) are available as reference standards, the assay was additionally validated for quantification purposes to study elimination of the drug and primary metabolite following a small oral dose of ketamine (50 mg) in 6 volunteers. Dehydronorketamine, a secondary metabolite, was also analyzed qualitatively to determine whether monitoring could improve retrospective detection of administration. The detection limit for ketamine and norketamine was 0.03 ng/mL and 0.05 ng/mL, respectively, and these compounds could be confirmed in urine for up to 5 and 6 days, respectively. Dehydronorketamine was confirmed up to 10 days, providing a very broad window of detection.
Source: PubMed
Detection of Ketamine and its Metabolites in Urine by Ultra High Pressure Liquid Chromatography–Tandem Mass Spectrometry
Authors: Parkin, M.C., Turfus, S.C., Smith, N.W., Halket, J.M., Braithwaite, R.A., Elliott, S.P., Osselton, D.M., Cowan, D.A. and Kicman, A.T.
Journal: Journal of Chromatography B
Volume: 876
Pages: 137-142
ISSN: 1570-0232
DOI: 10.1016/j.jchromb.2008.09.036
Abstract:Current analytical methods used for screening drugs and their metabolites in biological samples from victims of drug-facilitated sexual assault (DFSA) or other vulnerable groups can lack sufficient sensitivity. The application of liquid chromatography, employing small particle sizes, with tandem mass spectrometry (MS/MS) is likely to offer the sensitivity required for detecting candidate drugs and/or their metabolites in urine, as demonstrated here for ketamine. Ultra-performance liquid chromatography–mass spectrometry (UPLC–MS/MS) was performed following extraction of urine (4 mL) using mixed-mode (cation and C8) solid-phase cartridges. Only 20 μL of the 250 μL extract was injected, leaving sufficient volume for other assays important in DFSA cases. Three ion transitions were chosen for confirmatory purposes. As ketamine and norketamine (including their stable isotopes) are available as reference standards, the assay was additionally validated for quantification purposes to study elimination of the drug and primary metabolite following a small oral dose of ketamine (50 mg) in 6 volunteers. Dehydronorketamine, a secondary metabolite, was also analyzed qualitatively to determine whether monitoring could improve retrospective detection of administration. The detection limit for ketamine and norketamine was 0.03 ng/mL and 0.05 ng/mL, respectively, and these compounds could be confirmed in urine for up to 5 and 6 days, respectively. Dehydronorketamine was confirmed up to 10 days, providing a very broad window of detection.
Source: Manual
Preferred by: David Osselton
Detection of ketamine and its metabolites in urine by ultra high pressure liquid chromatography-tandem mass spectrometry.
Authors: Parkin, M.C., Turfus, S.C., Smith, N.W., Halket, J.M., Braithwaite, R.A., Elliott, S.P., Osselton, M.D., Cowan, D.A. and Kicman, A.T.
Journal: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
Volume: 876
Issue: 1
Pages: 137-142
eISSN: 1873-376X
ISSN: 1570-0232
DOI: 10.1016/j.jchromb.2008.09.036
Abstract:Current analytical methods used for screening drugs and their metabolites in biological samples from victims of drug-facilitated sexual assault (DFSA) or other vulnerable groups can lack sufficient sensitivity. The application of liquid chromatography, employing small particle sizes, with tandem mass spectrometry (MS/MS) is likely to offer the sensitivity required for detecting candidate drugs and/or their metabolites in urine, as demonstrated here for ketamine. Ultra-performance liquid chromatography-mass spectrometry (UPLC-MS/MS) was performed following extraction of urine (4 mL) using mixed-mode (cation and C8) solid-phase cartridges. Only 20 microL of the 250 microL extract was injected, leaving sufficient volume for other assays important in DFSA cases. Three ion transitions were chosen for confirmatory purposes. As ketamine and norketamine (including their stable isotopes) are available as reference standards, the assay was additionally validated for quantification purposes to study elimination of the drug and primary metabolite following a small oral dose of ketamine (50 mg) in 6 volunteers. Dehydronorketamine, a secondary metabolite, was also analyzed qualitatively to determine whether monitoring could improve retrospective detection of administration. The detection limit for ketamine and norketamine was 0.03 ng/mL and 0.05 ng/mL, respectively, and these compounds could be confirmed in urine for up to 5 and 6 days, respectively. Dehydronorketamine was confirmed up to 10 days, providing a very broad window of detection.
Source: Europe PubMed Central
Detection of ketamine and its metabolites in urine by ultra high pressure liquid chromatography-tandem mass spectrometry
Authors: PARKIN, M.C., TURFUS, S.C., SMITH, N.W., HALKET, J.M., BRAITHWAITE, R.A., ELLIOTT, S.P., OSSELTON, M.D., COWAN, D.A. and KICMAN, A.T.
Journal: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
Volume: 876
Issue: 1
Pages: 137-142
ISSN: 1570-0232
Source: CiNii EN
Detection of ketamine and its metabolites in urine by ultra high pressure liquid chromatography-tandem mass spectrometry
Authors: PARKIN, M.C., TURFUS, S.C., SMITH, N.W., HALKET, J.M., BRAITHWAITE, R.A., ELLIOTT, S.P., OSSELTON, M.D., COWAN, D.A. and KICMAN, A.T.
Journal: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
Volume: 876
Issue: 1
Pages: 137-142
ISSN: 1570-0232
Source: CiNii JP