Antibiofilm Activity of the Brown Alga Halidrys siliquosa against Clinically Relevant Human Pathogens

Authors: Busetti, A., Thompson, T.P., Tegazzini, D., Megaw, J., Maggs, C.A. and Gilmore, B.F.

http://eprints.bournemouth.ac.uk/24477/

Journal: Marine Drugs

Volume: 13

Issue: 6

Pages: 3581-3605

Publisher: MDPI AG

eISSN: 1660-3397

DOI: 10.3390/md13063581

The marine brown alga Halidrys siliquosa is known to produce compounds with antifouling activity against several marine bacteria. The aim of this study was to evaluate the antimicrobial and antibiofilm activity of organic extracts obtained from the marine brown alga H. siliquosa against a focused panel of clinically relevant human pathogens commonly associated with biofilm-related infections. The partially fractionated methanolic extract obtained from H. siliquosa collected along the shores of Co. Donegal; Ireland; displayed antimicrobial activity against bacteria of the genus Staphylococcus; Streptococcus; Enterococcus; Pseudomonas; Stenotrophomonas; and Chromobacterium with MIC and MBC values ranging from 0.0391 to 5 mg/mL. Biofilms of S. aureus MRSA were found to be susceptible to the algal methanolic extract with MBEC values ranging from 1.25 mg/mL to 5 mg/mL respectively. Confocal laser scanning microscopy using LIVE/DEAD staining confirmed the antimicrobial nature of the antibiofilm activity observed using the MBEC assay. A bioassay-guided fractionation method was developed yielding 10 active fractions from which to perform purification and structural elucidation of clinically-relevant antibiofilm compounds.

This data was imported from PubMed:

Authors: Busetti, A., Thompson, T.P., Tegazzini, D., Megaw, J., Maggs, C.A. and Gilmore, B.F.

http://eprints.bournemouth.ac.uk/24477/

Journal: Mar Drugs

Volume: 13

Issue: 6

Pages: 3581-3605

eISSN: 1660-3397

DOI: 10.3390/md13063581

The marine brown alga Halidrys siliquosa is known to produce compounds with antifouling activity against several marine bacteria. The aim of this study was to evaluate the antimicrobial and antibiofilm activity of organic extracts obtained from the marine brown alga H. siliquosa against a focused panel of clinically relevant human pathogens commonly associated with biofilm-related infections. The partially fractionated methanolic extract obtained from H. siliquosa collected along the shores of Co. Donegal; Ireland; displayed antimicrobial activity against bacteria of the genus Staphylococcus; Streptococcus; Enterococcus; Pseudomonas; Stenotrophomonas; and Chromobacterium with MIC and MBC values ranging from 0.0391 to 5 mg/mL. Biofilms of S. aureus MRSA were found to be susceptible to the algal methanolic extract with MBEC values ranging from 1.25 mg/mL to 5 mg/mL respectively. Confocal laser scanning microscopy using LIVE/DEAD staining confirmed the antimicrobial nature of the antibiofilm activity observed using the MBEC assay. A bioassay-guided fractionation method was developed yielding 10 active fractions from which to perform purification and structural elucidation of clinically-relevant antibiofilm compounds.

This data was imported from Scopus:

Authors: Busetti, A., Thompson, T.P., Tegazzini, D., Megaw, J., Maggs, C.A. and Gilmore, B.F.

http://eprints.bournemouth.ac.uk/24477/

Journal: Marine Drugs

Volume: 13

Issue: 6

Pages: 3581-3605

eISSN: 1660-3397

DOI: 10.3390/md13063581

© 2015 by the authors; licensee MDPI. The marine brown alga Halidrys siliquosa is known to produce compounds with antifouling activity against several marine bacteria. The aim of this study was to evaluate the antimicrobial and antibiofilm activity of organic extracts obtained from the marine brown alga H. siliquosa against a focused panel of clinically relevant human pathogens commonly associated with biofilm-related infections. The partially fractionated methanolic extract obtained from H. siliquosa collected along the shores of Co. Donegal; Ireland; displayed antimicrobial activity against bacteria of the genus Staphylococcus; Streptococcus; Enterococcus; Pseudomonas; Stenotrophomonas; and Chromobacterium with MIC and MBC values ranging from 0.0391 to 5 mg/mL. Biofilms of S. aureus MRSA were found to be susceptible to the algal methanolic extract with MBEC values ranging from 1.25 mg/mL to 5 mg/mL respectively. Confocal laser scanning microscopy using LIVE/DEAD staining confirmed the antimicrobial nature of the antibiofilm activity observed using the MBEC assay. A bioassay-guided fractionation method was developed yielding 10 active fractions from which to perform purification and structural elucidation of clinically-relevant antibiofilm compounds.

This data was imported from Web of Science (Lite):

Authors: Busetti, A., Thompson, T.P., Tegazzini, D., Megaw, J., Maggs, C.A. and Gilmore, B.F.

http://eprints.bournemouth.ac.uk/24477/

Journal: MARINE DRUGS

Volume: 13

Issue: 6

Pages: 3581-3605

ISSN: 1660-3397

DOI: 10.3390/md13063581

This data was imported from Europe PubMed Central:

Authors: Busetti, A., Thompson, T.P., Tegazzini, D., Megaw, J., Maggs, C.A. and Gilmore, B.F.

http://eprints.bournemouth.ac.uk/24477/

Journal: Marine drugs

Volume: 13

Issue: 6

Pages: 3581-3605

eISSN: 1660-3397

The marine brown alga Halidrys siliquosa is known to produce compounds with antifouling activity against several marine bacteria. The aim of this study was to evaluate the antimicrobial and antibiofilm activity of organic extracts obtained from the marine brown alga H. siliquosa against a focused panel of clinically relevant human pathogens commonly associated with biofilm-related infections. The partially fractionated methanolic extract obtained from H. siliquosa collected along the shores of Co. Donegal; Ireland; displayed antimicrobial activity against bacteria of the genus Staphylococcus; Streptococcus; Enterococcus; Pseudomonas; Stenotrophomonas; and Chromobacterium with MIC and MBC values ranging from 0.0391 to 5 mg/mL. Biofilms of S. aureus MRSA were found to be susceptible to the algal methanolic extract with MBEC values ranging from 1.25 mg/mL to 5 mg/mL respectively. Confocal laser scanning microscopy using LIVE/DEAD staining confirmed the antimicrobial nature of the antibiofilm activity observed using the MBEC assay. A bioassay-guided fractionation method was developed yielding 10 active fractions from which to perform purification and structural elucidation of clinically-relevant antibiofilm compounds.

The data on this page was last updated at 04:53 on December 15, 2018.