Antibiofilm activity of the brown alga Halidrys siliquosa against clinically relevant human pathogens
Authors: Busetti, A., Thompson, T.P., Tegazzini, D., Megaw, J., Maggs, C.A. and Gilmore, B.F.
Journal: Marine Drugs
Volume: 13
Issue: 6
Pages: 3581-3605
eISSN: 1660-3397
DOI: 10.3390/md13063581
Abstract:The marine brown alga Halidrys siliquosa is known to produce compounds with antifouling activity against several marine bacteria. The aim of this study was to evaluate the antimicrobial and antibiofilm activity of organic extracts obtained from the marine brown alga H. siliquosa against a focused panel of clinically relevant human pathogens commonly associated with biofilm-related infections. The partially fractionated methanolic extract obtained from H. siliquosa collected along the shores of Co. Donegal; Ireland; displayed antimicrobial activity against bacteria of the genus Staphylococcus; Streptococcus; Enterococcus; Pseudomonas; Stenotrophomonas; and Chromobacterium with MIC and MBC values ranging from 0.0391 to 5 mg/mL. Biofilms of S. aureus MRSA were found to be susceptible to the algal methanolic extract with MBEC values ranging from 1.25 mg/mL to 5 mg/mL respectively. Confocal laser scanning microscopy using LIVE/DEAD staining confirmed the antimicrobial nature of the antibiofilm activity observed using the MBEC assay. A bioassay-guided fractionation method was developed yielding 10 active fractions from which to perform purification and structural elucidation of clinically-relevant antibiofilm compounds.
https://eprints.bournemouth.ac.uk/24477/
Source: Scopus
Antibiofilm Activity of the Brown Alga Halidrys siliquosa against Clinically Relevant Human Pathogens.
Authors: Busetti, A., Thompson, T.P., Tegazzini, D., Megaw, J., Maggs, C.A. and Gilmore, B.F.
Journal: Mar Drugs
Volume: 13
Issue: 6
Pages: 3581-3605
eISSN: 1660-3397
DOI: 10.3390/md13063581
Abstract:The marine brown alga Halidrys siliquosa is known to produce compounds with antifouling activity against several marine bacteria. The aim of this study was to evaluate the antimicrobial and antibiofilm activity of organic extracts obtained from the marine brown alga H. siliquosa against a focused panel of clinically relevant human pathogens commonly associated with biofilm-related infections. The partially fractionated methanolic extract obtained from H. siliquosa collected along the shores of Co. Donegal; Ireland; displayed antimicrobial activity against bacteria of the genus Staphylococcus; Streptococcus; Enterococcus; Pseudomonas; Stenotrophomonas; and Chromobacterium with MIC and MBC values ranging from 0.0391 to 5 mg/mL. Biofilms of S. aureus MRSA were found to be susceptible to the algal methanolic extract with MBEC values ranging from 1.25 mg/mL to 5 mg/mL respectively. Confocal laser scanning microscopy using LIVE/DEAD staining confirmed the antimicrobial nature of the antibiofilm activity observed using the MBEC assay. A bioassay-guided fractionation method was developed yielding 10 active fractions from which to perform purification and structural elucidation of clinically-relevant antibiofilm compounds.
https://eprints.bournemouth.ac.uk/24477/
Source: PubMed
Antibiofilm Activity of the Brown Alga <i>Halidrys siliquosa</i> against Clinically Relevant Human Pathogens
Authors: Busetti, A., Thompson, T.P., Tegazzini, D., Megaw, J., Maggs, C.A. and Gilmore, B.F.
Journal: MARINE DRUGS
Volume: 13
Issue: 6
Pages: 3581-3605
eISSN: 1660-3397
DOI: 10.3390/md13063581
https://eprints.bournemouth.ac.uk/24477/
Source: Web of Science (Lite)
Antibiofilm activity of the brown alga Halidrys siliquosa against clinically relevant human pathogens
Authors: Busetti, A., Thompson, T.P., Tegazzini, D., Megaw, J., Maggs, C.A. and Gilmore, B.F.
Journal: Marine Drugs
Volume: 13
Issue: 6
Pages: 3581-3605
Publisher: MDPI AG
eISSN: 1660-3397
DOI: 10.3390/md13063581
Abstract:The marine brown alga Halidrys siliquosa is known to produce compounds with antifouling activity against several marine bacteria. The aim of this study was to evaluate the antimicrobial and antibiofilm activity of organic extracts obtained from the marine brown alga H. siliquosa against a focused panel of clinically relevant human pathogens commonly associated with biofilm-related infections. The partially fractionated methanolic extract obtained from H. siliquosa collected along the shores of Co. Donegal; Ireland; displayed antimicrobial activity against bacteria of the genus Staphylococcus; Streptococcus; Enterococcus; Pseudomonas; Stenotrophomonas; and Chromobacterium with MIC and MBC values ranging from 0.0391 to 5 mg/mL. Biofilms of S. aureus MRSA were found to be susceptible to the algal methanolic extract with MBEC values ranging from 1.25 mg/mL to 5 mg/mL respectively. Confocal laser scanning microscopy using LIVE/DEAD staining confirmed the antimicrobial nature of the antibiofilm activity observed using the MBEC assay. A bioassay-guided fractionation method was developed yielding 10 active fractions from which to perform purification and structural elucidation of clinically-relevant antibiofilm compounds.
https://eprints.bournemouth.ac.uk/24477/
Source: Manual
Antibiofilm Activity of the Brown Alga Halidrys siliquosa against Clinically Relevant Human Pathogens.
Authors: Busetti, A., Thompson, T.P., Tegazzini, D., Megaw, J., Maggs, C.A. and Gilmore, B.F.
Journal: Marine drugs
Volume: 13
Issue: 6
Pages: 3581-3605
eISSN: 1660-3397
ISSN: 1660-3397
DOI: 10.3390/md13063581
Abstract:The marine brown alga Halidrys siliquosa is known to produce compounds with antifouling activity against several marine bacteria. The aim of this study was to evaluate the antimicrobial and antibiofilm activity of organic extracts obtained from the marine brown alga H. siliquosa against a focused panel of clinically relevant human pathogens commonly associated with biofilm-related infections. The partially fractionated methanolic extract obtained from H. siliquosa collected along the shores of Co. Donegal; Ireland; displayed antimicrobial activity against bacteria of the genus Staphylococcus; Streptococcus; Enterococcus; Pseudomonas; Stenotrophomonas; and Chromobacterium with MIC and MBC values ranging from 0.0391 to 5 mg/mL. Biofilms of S. aureus MRSA were found to be susceptible to the algal methanolic extract with MBEC values ranging from 1.25 mg/mL to 5 mg/mL respectively. Confocal laser scanning microscopy using LIVE/DEAD staining confirmed the antimicrobial nature of the antibiofilm activity observed using the MBEC assay. A bioassay-guided fractionation method was developed yielding 10 active fractions from which to perform purification and structural elucidation of clinically-relevant antibiofilm compounds.
https://eprints.bournemouth.ac.uk/24477/
Source: Europe PubMed Central
Antibiofilm Activity of the Brown Alga Halidrys siliquosa against Clinically Relevant Human Pathogens.
Authors: Busetti, A., Thompson, T.P., Tegazzini, D., Megaw, J., Maggs, C. and Gilmore, B.F.
Journal: Marine Drugs
Volume: 13
Issue: 6
Pages: 3581-3605
ISSN: 1660-3397
Abstract:The marine brown alga Halidrys siliquosa is known to produce compounds with antifouling activity against several marine bacteria. The aim of this study was to evaluate the antimicrobial and antibiofilm activity of organic extracts obtained from the marine brown alga H. siliquosa against a focused panel of clinically relevant human pathogens commonly associated with biofilm-related infections. The partially fractionated methanolic extract obtained from H. siliquosa collected along the shores of Co. Donegal; Ireland; displayed antimicrobial activity against bacteria of the genus Staphylococcus; Streptococcus; Enterococcus; Pseudomonas; Stenotrophomonas; and Chromobacterium with MIC and MBC values ranging from 0.0391 to 5 mg/mL. Biofilms of S. aureus MRSA were found to be susceptible to the algal methanolic extract with MBEC values ranging from 1.25 mg/mL to 5 mg/mL respectively. Confocal laser scanning microscopy using LIVE/DEAD staining confirmed the antimicrobial nature of the antibiofilm activity observed using the MBEC assay. A bioassay-guided fractionation method was developed yielding 10 active fractions from which to perform purification and structural elucidation of clinically-relevant antibiofilm compounds.
https://eprints.bournemouth.ac.uk/24477/
Source: BURO EPrints