DNA Isolation Protocol for Red Seaweed “Rhodophyta”

Authors: Wattier, R.A., Prodohl, P.A. and Maggs, C.A.

Journal: Plant Molecular Biology Reporter

Volume: 18

Issue: 3

Pages: 275-281

ISSN: 0735-9640

DOI: 10.1007/BF02823999

Abstract:

We report a DNA isolation protocol for red seaweed. The method is a modification of the Dellaporta et al. “1983” protocol for land plants. Our simplified version can be used to process large sample numbers and to minimise polysaccharide co-isolation. The protocol was applied to 12 red seaweed species as well as one green alga and one land plant. The protocol yields about 5 μg of high molecular weight DNA from 10 mg of dried material, with no RNA. No sign of degradation was observed after agarose gel electrophoresis for both freshly extracted DNA and DNA stored for 18 months at 4°C. DNA isolated by our protocol was suitable for genomic library construction “tested for one species”, endonuclease restriction, and PCR amplification for all species.

Source: Scopus