Some rumen ciliates have endosymbiotic methanogens
Authors: Finlay, B.J., Esteban, G., Clarke, K.J., Williams, A.G., Embley, T.M. and Hirt, R.P.
Journal: FEMS Microbiology Letters
Volume: 117
Issue: 2
Pages: 157-161
eISSN: 1574-6968
ISSN: 0378-1097
DOI: 10.1111/j.1574-6968.1994.tb06758.x
Abstract:Most of the small ciliate protozoa, including Dasytricha ruminantium and Entodinium spp. living in the rumen of sheep, were found to have intracellular bacteria. These bacteria were not present in digestive vacuoles. They showed characteristic coenzyme F420 autofluorescence and they were detected with a rhodamine-labelled Archaea-specific oligonucleotide probe. The measured volume percent of autofluorescing bacteria (1%) was close to the total volume of intracellular bacteria estimated from TEM stereology. Thus it is likely that all of the bacteria living in the cytoplasm of these ciliates were endosymbiotic methanogens, using H2 evolved by the host ciliate to form methane. Intracellular methanogens appear to be much more numerous than those attached to the external cell surface of ciliates. © 1994.
Source: Scopus
Some rumen ciliates have endosymbiotic methanogens.
Authors: Finlay, B.J., Esteban, G., Clarke, K.J., Williams, A.G., Embley, T.M. and Hirt, R.P.
Journal: FEMS Microbiol Lett
Volume: 117
Issue: 2
Pages: 157-161
ISSN: 0378-1097
DOI: 10.1111/j.1574-6968.1994.tb06758.x
Abstract:Most of the small ciliate protozoa, including Dasytricha ruminantium and Entodinium spp. living in the rumen of sheep, were found to have intracellular bacteria. These bacteria were not present in digestive vacuoles. They showed characteristic coenzyme F420 autofluorescence and they were detected with a rhodamine-labelled Archaea-specific oligonucleotide probe. The measured volume percent of autofluorescing bacteria (1%) was close to the total volume of intracellular bacteria estimated from TEM stereology. Thus it is likely that all of the bacteria living in the cytoplasm of these ciliates were endosymbiotic methanogens, using H2 evolved by the host ciliate to form methane. Intracellular methanogens appear to be much more numerous than those attached to the external cell surface of ciliates.
Source: PubMed
SOME RUMEN CILIATES HAVE ENDOSYMBIOTIC METHANOGENS
Authors: FINLAY, B.J., ESTEBAN, G., CLARKE, K.J., WILLIAMS, A.G., EMBLEY, T.M. and HIRT, R.P.
Journal: FEMS MICROBIOLOGY LETTERS
Volume: 117
Issue: 2
Pages: 157-162
ISSN: 0378-1097
DOI: 10.1016/0378-1097(94)90188-0
Source: Web of Science (Lite)
Some rumen ciliates have endosymbiotic methanogens
Authors: Finlay, B.J., Esteban, G., Clarke, K.J., Williams, A.G., Embley, T.M. and Hirt, R.
Journal: FEMS Microbiology Letters
Volume: 117
Issue: 2
Pages: 157-161
ISSN: 1574-6968
Abstract:Most of the small ciliate protozoa, including Dasytricha ruminantium and Entodinium spp. living in the rumen of sheep, were found to have intracellular bacteria. These bacteria were not present in digestive vacuoles. They showed characteristic coenzyme F420 autofluorescence and they were detected with a rhodamine-labelled Archaea-specific oligonucleotide probe. The measured volume percent of autofluorescing bacteria (1%) was close to the total volume of intracellular bacteria estimated from TEM stereology. Thus it is likely that all of the bacteria living in the cytoplasm of these ciliates were endosymbiotic methanogens, using H2 evolved by the host ciliate to form methane. Intracellular methanogens appear to be much more numerous than those attached to the external cell surface of ciliates.
Source: Manual
Some rumen ciliates have endosymbiotic methanogens.
Authors: Finlay, B.J., Esteban, G., Clarke, K.J., Williams, A.G., Embley, T.M. and Hirt, R.P.
Journal: FEMS microbiology letters
Volume: 117
Issue: 2
Pages: 157-161
eISSN: 1574-6968
ISSN: 0378-1097
DOI: 10.1111/j.1574-6968.1994.tb06758.x
Abstract:Most of the small ciliate protozoa, including Dasytricha ruminantium and Entodinium spp. living in the rumen of sheep, were found to have intracellular bacteria. These bacteria were not present in digestive vacuoles. They showed characteristic coenzyme F420 autofluorescence and they were detected with a rhodamine-labelled Archaea-specific oligonucleotide probe. The measured volume percent of autofluorescing bacteria (1%) was close to the total volume of intracellular bacteria estimated from TEM stereology. Thus it is likely that all of the bacteria living in the cytoplasm of these ciliates were endosymbiotic methanogens, using H2 evolved by the host ciliate to form methane. Intracellular methanogens appear to be much more numerous than those attached to the external cell surface of ciliates.
Source: Europe PubMed Central