Somatic recombination in Rhynchosporium secalis
Authors: NEWTON, A.C.
Journal: Plant Pathology
Volume: 38
Issue: 1
Pages: 71-74
eISSN: 1365-3059
ISSN: 0032-0862
DOI: 10.1111/j.1365-3059.1989.tb01429.x
Abstract:Drug‐resistant mutants were induced in strains of Rhynchosporium secalis. Two mutants resistant to hygromycin‐B and 5‐fluorouracil, respectively, were stable and easily selected in culture. Mixtures of the two mutants failed to yield any recombinants, whether under selection pressure or not, on agar and on barley leaves. After protoplast production and fusion, followed by selection on media containing both drugs, two recombinants were isolated. Single‐spore derivatives expressed the drug resistances of both parental strains. Copyright © 1989, Wiley Blackwell. All rights reserved
Source: Scopus