Attempted somatic hybridization of Puccinia striiformis f. sp. tritici and P. striiformis f. sp. hordei
Authors: NEWTON, A.C., JOHNSON, R. and CATEN, C.E.
Journal: Plant Pathology
Volume: 35
Issue: 1
Pages: 108-113
eISSN: 1365-3059
ISSN: 0032-0862
DOI: 10.1111/j.1365-3059.1986.tb01988.x
Abstract:Attempts were made to produce somatic hybrids between isolates of Puccinia striiformis f. sp. tritici and f. sp. hordei. A mixed infection was produced on a common susceptible barley host, Fong Tien, using white‐spored isolates of P. striiformis f. sp. tritici and yellow‐spored isolates off. sp. hordei. Selection was made for non‐parental spore colour on selective wheat and barley hosts, and variants thus isolated were analysed for virulence markers, and for isozyme and double‐stranded RNA (dsRNA) markers, all of which clearly differentiated the parental isolates. Two white‐spored (non‐parental) isolates were found on the selective barley host which otherwise resembled the parental f. sp. hordei isolate in virulence, isozyme and dsRNA markers. The most likely explanation of the origin of these isolates is mutation to white spore colour in the f. sp. hordei isolate. Copyright © 1986, Wiley Blackwell. All rights reserved
Source: Scopus