Transformation of the plant pathogenic fungus, Rhynchosporium secalis
Authors: Rohe, M., Searle, J., Newton, A.C. and Knogge, W.
Journal: Current Genetics
Volume: 29
Issue: 6
Pages: 587-590
ISSN: 0172-8083
DOI: 10.1007/BF02426964
Abstract:The barley leaf scald fungus, Rhynchosporium secalis, was transformed to hygromycin-B and phleomycin resistance using the hph gene from E. coli and the ble gene from Streptoalloteichus hindustanus under the control of Aspergillus nidulans promoter and terminator sequences, Plasmid DNA was introduced into fungal protoplasts by PEG/CaCl2 treatment. Transformation frequencies varied from 59 to 493 transformants per 10 μg of DNA and 5 x 107 protoplasts. The antibiotic-resistant phenotype appeared to be stable under selective, as well as under nonselective, conditions for several generations. Co-transformation using the E. coli uidA gene under the control of A. nidulans promoter and terminator sequences on a non-selectable plasmid occurred at frequencies of up to 66%.
Source: Scopus
Transformation of the plant pathogenic fungus, Rhynchosporium secalis.
Authors: Rohe, M., Searle, J., Newton, A.C. and Knogge, W.
Journal: Curr Genet
Volume: 29
Issue: 6
Pages: 587-590
ISSN: 0172-8083
DOI: 10.1007/BF02426964
Abstract:The barley leaf scald fungus, Rhynchosporium secalis, was transformed to hygromycin-B and phleomycin resistance using the hph gene from E. coli and the ble gene from Streptoalloteichus hindustanus under the control of Aspergillus nidulans promoter and terminator sequences. Plasmid DNA was introduced into fungal protoplasts by PEG/CaCl2 treatment. Transformation frequencies varied from 59 to 493 transformants per 10 microg of DNA and 5 x 10(7) protoplasts. The antibiotic-resistant phenotype appeared to be stable under selective, as well as under non-selective, conditions for several generations. Co-transformation using the E. coli uidA gene under the control of A. nidulans promoter and terminator sequences on a non-selectable plasmid occurred at frequencies of up to 66%.
Source: PubMed
Transformation of the plant pathogenic fungus, Rhynchosporium secalis.
Authors: Rohe, M., Searle, J., Newton, A.C. and Knogge, W.
Journal: Current genetics
Volume: 29
Issue: 6
Pages: 587-590
eISSN: 1432-0983
ISSN: 0172-8083
DOI: 10.1007/bf02426964
Abstract:The barley leaf scald fungus, Rhynchosporium secalis, was transformed to hygromycin-B and phleomycin resistance using the hph gene from E. coli and the ble gene from Streptoalloteichus hindustanus under the control of Aspergillus nidulans promoter and terminator sequences. Plasmid DNA was introduced into fungal protoplasts by PEG/CaCl2 treatment. Transformation frequencies varied from 59 to 493 transformants per 10 microg of DNA and 5 x 10(7) protoplasts. The antibiotic-resistant phenotype appeared to be stable under selective, as well as under non-selective, conditions for several generations. Co-transformation using the E. coli uidA gene under the control of A. nidulans promoter and terminator sequences on a non-selectable plasmid occurred at frequencies of up to 66%.
Source: Europe PubMed Central