Dielectrophoresis of Caenorhabditis elegans

Authors: Chuang, H.S., Raizen, D.M., Lamb, A., Dabbish, N. and Bau, H.H.

Journal: Lab on a Chip

Volume: 11

Issue: 4

Pages: 599-604

eISSN: 1473-0189

ISSN: 1473-0197

DOI: 10.1039/c0lc00532k

Abstract:

We demonstrate for the first time the dielectrophoretic trapping and manipulation of a whole animal, the nematode Caenorhabditis elegans. We studied the effect of the electric field on the nematode as a function of field intensity and frequency. We identified a range of electric field intensities and frequencies that trap worms without apparent adverse effect on their viability. Worms tethered by dielectrophoresis (DEP) exhibit behavioral responses to blue light, indicating that at least some of the nervous system functions are unimpaired by the electrical field. DEP is useful to dynamically tether nematodes, sort nematodes according to size, and separate dead worms from live ones. © 2011 The Royal Society of Chemistry.

Source: Scopus

Dielectrophoresis of Caenorhabditis elegans.

Authors: Chuang, H.-S., Raizen, D.M., Lamb, A., Dabbish, N. and Bau, H.H.

Journal: Lab Chip

Volume: 11

Issue: 4

Pages: 599-604

eISSN: 1473-0189

DOI: 10.1039/c0lc00532k

Abstract:

We demonstrate for the first time the dielectrophoretic trapping and manipulation of a whole animal, the nematode Caenorhabditis elegans. We studied the effect of the electric field on the nematode as a function of field intensity and frequency. We identified a range of electric field intensities and frequencies that trap worms without apparent adverse effect on their viability. Worms tethered by dielectrophoresis (DEP) exhibit behavioral responses to blue light, indicating that at least some of the nervous system functions are unimpaired by the electrical field. DEP is useful to dynamically tether nematodes, sort nematodes according to size, and separate dead worms from live ones.

Source: PubMed

Dielectrophoresis of <i>Caenorhabditis elegans</i>

Authors: Chuang, H.-S., Raizen, D.M., Lamb, A., Dabbish, N. and Bau, H.H.

Journal: LAB ON A CHIP

Volume: 11

Issue: 4

Pages: 599-604

eISSN: 1473-0189

ISSN: 1473-0197

DOI: 10.1039/c0lc00532k

Source: Web of Science (Lite)

Dielectrophoresis of Caenorhabditis elegans.

Authors: Chuang, H.-S., Raizen, D.M., Lamb, A., Dabbish, N. and Bau, H.H.

Journal: Lab on a chip

Volume: 11

Issue: 4

Pages: 599-604

eISSN: 1473-0189

ISSN: 1473-0197

DOI: 10.1039/c0lc00532k

Abstract:

We demonstrate for the first time the dielectrophoretic trapping and manipulation of a whole animal, the nematode Caenorhabditis elegans. We studied the effect of the electric field on the nematode as a function of field intensity and frequency. We identified a range of electric field intensities and frequencies that trap worms without apparent adverse effect on their viability. Worms tethered by dielectrophoresis (DEP) exhibit behavioral responses to blue light, indicating that at least some of the nervous system functions are unimpaired by the electrical field. DEP is useful to dynamically tether nematodes, sort nematodes according to size, and separate dead worms from live ones.

Source: Europe PubMed Central