Simultaneous assessment of aneuploidy, polymorphisms, and mitochondrial DNA content in human polar bodies and embryos with the use of a novel microarray platform.
Authors: Konstantinidis, M., Alfarawati, S., Hurd, D., Paolucci, M., Shovelton, J., Fragouli, E. and Wells, D.
Journal: Fertil Steril
Volume: 102
Issue: 5
Pages: 1385-1392
eISSN: 1556-5653
DOI: 10.1016/j.fertnstert.2014.07.1233
Abstract:OBJECTIVE: To develop a microarray platform that allows simultaneous assessment of aneuploidy and quantification of mitochondrial DNA (mtDNA) in human polar bodies and embryos. DESIGN: Optimization and validation applied to cell lines and clinical samples (polar bodies, blastomeres, and trophectoderm biopsies). SETTING: University research laboratory and a preimplantation genetic diagnosis (PGD) reference laboratory. PATIENT(S): Samples from 65 couples who underwent PGD for aneuploidy and/or a single-gene disorder. INTERVENTION(S): None. MAIN OUTCOME MEASURE(S): 1) Comparison of aneuploidy screening results obtained with the use of the new microarray with those derived from two well established cytogenetic techniques. 2) mtDNA quantification. 3) Analysis of single-nucleotide polymorphisms. RESULT(S): The fully optimized microarray was estimated to have an accuracy of ≥97% for the detection of individual aneuploidies and to detect 99% of chromosomally abnormal embryos. The microarray was shown to accurately determine relative quantities of mtDNA. Information provided from polymorphic loci was sufficient to allow confirmation that an embryo was derived from specific parents. CONCLUSION(S): It is hoped that methods such as those reported here, which provide information on several aspects of oocyte/embryo genetics, could lead to improved strategies for identifying viable embryos, thereby increasing the likelihood of successful implantation. Additionally, the provision of genotyping information has the potential to reveal DNA contaminants and confirm parental origin of embryos.
Source: PubMed
Simultaneous assessment of aneuploidy, polymorphisms, and mitochondrial DNA content in human polar bodies and embryos with the use of a novel microarray platform
Authors: Konstantinidis, M., Alfarawati, S., Hurd, D., Paolucci, M., Shovelton, J., Fragouli, E. and Wells, D.
Journal: FERTILITY AND STERILITY
Volume: 102
Issue: 5
eISSN: 1556-5653
ISSN: 0015-0282
DOI: 10.1016/j.fertnstert.2014.07.1233
Source: Web of Science (Lite)
Simultaneous assessment of aneuploidy, polymorphisms, and mitochondrial DNA content in human polar bodies and embryos with the use of a novel microarray platform
Authors: Konstantinidis, M., Alfarawati, S., Hurd, D., Paolucci, D., Shovelton, J., Fragkouli, E. and Wells, D.
Journal: Fertility and Sterility
Volume: 102
Issue: 5
Pages: 1385-1392
Publisher: Elsevier
ISSN: 0015-0282
Source: Manual
Simultaneous assessment of aneuploidy, polymorphisms, and mitochondrial DNA content in human polar bodies and embryos with the use of a novel microarray platform.
Authors: Konstantinidis, M., Alfarawati, S., Hurd, D., Paolucci, M., Shovelton, J., Fragouli, E. and Wells, D.
Journal: Fertility and sterility
Volume: 102
Issue: 5
Pages: 1385-1392
eISSN: 1556-5653
ISSN: 0015-0282
DOI: 10.1016/j.fertnstert.2014.07.1233
Abstract:Objective
To develop a microarray platform that allows simultaneous assessment of aneuploidy and quantification of mitochondrial DNA (mtDNA) in human polar bodies and embryos.Design
Optimization and validation applied to cell lines and clinical samples (polar bodies, blastomeres, and trophectoderm biopsies).Setting
University research laboratory and a preimplantation genetic diagnosis (PGD) reference laboratory.Patient(s)
Samples from 65 couples who underwent PGD for aneuploidy and/or a single-gene disorder.Intervention(s)
None.Main outcome measure(s)
1) Comparison of aneuploidy screening results obtained with the use of the new microarray with those derived from two well established cytogenetic techniques. 2) mtDNA quantification. 3) Analysis of single-nucleotide polymorphisms.Result(s)
The fully optimized microarray was estimated to have an accuracy of ≥97% for the detection of individual aneuploidies and to detect 99% of chromosomally abnormal embryos. The microarray was shown to accurately determine relative quantities of mtDNA. Information provided from polymorphic loci was sufficient to allow confirmation that an embryo was derived from specific parents.Conclusion(s)
It is hoped that methods such as those reported here, which provide information on several aspects of oocyte/embryo genetics, could lead to improved strategies for identifying viable embryos, thereby increasing the likelihood of successful implantation. Additionally, the provision of genotyping information has the potential to reveal DNA contaminants and confirm parental origin of embryos.Source: Europe PubMed Central