Data-driven strategy for the discovery of potential urinary biomarkers of habitual dietary exposure

Authors: Lloyd, A.J., Beckmann, M., Haldar, S., Seal, C., Brandt, K. and Draper, J.

Journal: American Journal of Clinical Nutrition

Volume: 97

Issue: 2

Pages: 377-389

eISSN: 1938-3207

ISSN: 0002-9165

DOI: 10.3945/ajcn.112.048033

Abstract:

Background: An understanding of causal relations between diet and health is hindered by the lack of robust biological markers of food exposure. Objective: We aimed to develop a data-driven procedure to discover urine biomarkers indicative of habitual exposure to different foods. Design: The habitual diet of 68 participants was assessed by using 4 food-frequency questionnaires over 3 mo, and participants were assigned to different consumption-frequency classes for 58 dietary components. Flow infusion electrospray-ionization mass spectrom-etry followed by supervised multivariate data analysis was used to determine whether the chemical composition of urine was related to specific differences in the consumption levels of each food. Results: Foods were eaten habitually in 1 of 5 basic patterns differing in range and distribution of consumption frequency. Overnight, 24-h, and fasting urine samples proved useful for biomarker lead discovery with habitual citrus exposure used as a paradigm. Exposure level discrimination robustness improved linearly as urine samples from low-frequency citrus consumers were compared with urine samples from participants reporting increasingly higher intakes. For all foods, distinctiveness and consumption-frequency range influenced the likelihood that differential dietary exposure could be detected. Model output statistics indicated foods for which biomarker lead discovery was feasible. Metabolites proposed previously as acute intake biomarkers of citrus (proline betaine), oily fish (methylhistidine), coffee (dihydrocaffeic acid derivatives), and tomato (phenolic metabolites) were also biomarkers of habitual exposure. A significance threshold in modeling output statistics was determined to guide the discovery of potential biomarkers for other foods. Conclusion: This data-driven strategy can identify urinary metabolites associated with habitual exposure to specific foods. © 2013 American Society for Nutrition.

Source: Scopus

Data-driven strategy for the discovery of potential urinary biomarkers of habitual dietary exposure.

Authors: Lloyd, A.J., Beckmann, M., Haldar, S., Seal, C., Brandt, K. and Draper, J.

Journal: Am J Clin Nutr

Volume: 97

Issue: 2

Pages: 377-389

eISSN: 1938-3207

DOI: 10.3945/ajcn.112.048033

Abstract:

BACKGROUND: An understanding of causal relations between diet and health is hindered by the lack of robust biological markers of food exposure. OBJECTIVE: We aimed to develop a data-driven procedure to discover urine biomarkers indicative of habitual exposure to different foods. DESIGN: The habitual diet of 68 participants was assessed by using 4 food-frequency questionnaires over 3 mo, and participants were assigned to different consumption-frequency classes for 58 dietary components. Flow infusion electrospray-ionization mass spectrometry followed by supervised multivariate data analysis was used to determine whether the chemical composition of urine was related to specific differences in the consumption levels of each food. RESULTS: Foods were eaten habitually in 1 of 5 basic patterns differing in range and distribution of consumption frequency. Overnight, 24-h, and fasting urine samples proved useful for biomarker lead discovery with habitual citrus exposure used as a paradigm. Exposure level discrimination robustness improved linearly as urine samples from low-frequency citrus consumers were compared with urine samples from participants reporting increasingly higher intakes. For all foods, distinctiveness and consumption-frequency range influenced the likelihood that differential dietary exposure could be detected. Model output statistics indicated foods for which biomarker lead discovery was feasible. Metabolites proposed previously as acute intake biomarkers of citrus (proline betaine), oily fish (methylhistidine), coffee (dihydrocaffeic acid derivatives), and tomato (phenolic metabolites) were also biomarkers of habitual exposure. A significance threshold in modeling output statistics was determined to guide the discovery of potential biomarkers for other foods. CONCLUSION: This data-driven strategy can identify urinary metabolites associated with habitual exposure to specific foods. This trial has the UK registration number 4349 and was registered at isrtcn.org as CCT-NAPN-A13175.

Source: PubMed

Data-driven strategy for the discovery of potential urinary biomarkers of habitual dietary exposure

Authors: Lloyd, A.J., Beckmann, M., Haldar, S., Seal, C., Brandt, K. and Draper, J.

Journal: AMERICAN JOURNAL OF CLINICAL NUTRITION

Volume: 97

Issue: 2

Pages: 377-389

eISSN: 1938-3207

ISSN: 0002-9165

DOI: 10.3945/ajcn.112.048033

Source: Web of Science (Lite)

Data-driven strategy for the discovery of potential urinary biomarkers of habitual dietary exposure.

Authors: Lloyd, A.J., Beckmann, M., Haldar, S., Seal, C., Brandt, K. and Draper, J.

Journal: The American journal of clinical nutrition

Volume: 97

Issue: 2

Pages: 377-389

eISSN: 1938-3207

ISSN: 0002-9165

DOI: 10.3945/ajcn.112.048033

Abstract:

Background

An understanding of causal relations between diet and health is hindered by the lack of robust biological markers of food exposure.

Objective

We aimed to develop a data-driven procedure to discover urine biomarkers indicative of habitual exposure to different foods.

Design

The habitual diet of 68 participants was assessed by using 4 food-frequency questionnaires over 3 mo, and participants were assigned to different consumption-frequency classes for 58 dietary components. Flow infusion electrospray-ionization mass spectrometry followed by supervised multivariate data analysis was used to determine whether the chemical composition of urine was related to specific differences in the consumption levels of each food.

Results

Foods were eaten habitually in 1 of 5 basic patterns differing in range and distribution of consumption frequency. Overnight, 24-h, and fasting urine samples proved useful for biomarker lead discovery with habitual citrus exposure used as a paradigm. Exposure level discrimination robustness improved linearly as urine samples from low-frequency citrus consumers were compared with urine samples from participants reporting increasingly higher intakes. For all foods, distinctiveness and consumption-frequency range influenced the likelihood that differential dietary exposure could be detected. Model output statistics indicated foods for which biomarker lead discovery was feasible. Metabolites proposed previously as acute intake biomarkers of citrus (proline betaine), oily fish (methylhistidine), coffee (dihydrocaffeic acid derivatives), and tomato (phenolic metabolites) were also biomarkers of habitual exposure. A significance threshold in modeling output statistics was determined to guide the discovery of potential biomarkers for other foods.

Conclusion

This data-driven strategy can identify urinary metabolites associated with habitual exposure to specific foods. This trial has the UK registration number 4349 and was registered at isrtcn.org as CCT-NAPN-A13175.

Source: Europe PubMed Central