Housekeeping genes for cryopreservation studies on zebrafish embryos and blastomeres

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This data was imported from PubMed:

Authors: Lin, C., Spikings, E., Zhang, T. and Rawson, D.

Journal: Theriogenology

Volume: 71

Issue: 7

Pages: 1147-1155

ISSN: 0093-691X

DOI: 10.1016/j.theriogenology.2008.12.013

Cryopreservation success is usually analysed in terms of cell survival, although there are other potential effects that do not necessarily result in cell death. These include DNA damage, which could result in altered gene expression. Real-time reverse transcriptase PCR allows quantitative analysis of gene expression but usually requires analysis of a 'housekeeping' gene as an internal reference. As the stability of housekeeping genes varies significantly among different groups of samples, it is recommended that those chosen are validated for each different type of sample group. This study aimed to validate housekeeping genes for use in cryopreservation studies of zebrafish embryos. Seven potential housekeeping genes were analysed across fresh and chilled intact embryos and across fresh and frozen isolated blastomeres using the GeNorm and NormFinder software packages. Results suggest that combined use of beta-actin and EF1alpha as housekeeping genes would be suitable for cryopreservation studies on zebrafish embryos and blastomeres.

This data was imported from Scopus:

Authors: Lin, C., Spikings, E., Zhang, T. and Rawson, D.

Journal: Theriogenology

Volume: 71

Issue: 7

Pages: 1147-1155

ISSN: 0093-691X

DOI: 10.1016/j.theriogenology.2008.12.013

Cryopreservation success is usually analysed in terms of cell survival, although there are other potential effects that do not necessarily result in cell death. These include DNA damage, which could result in altered gene expression. Real-time reverse transcriptase PCR allows quantitative analysis of gene expression but usually requires analysis of a 'housekeeping' gene as an internal reference. As the stability of housekeeping genes varies significantly among different groups of samples, it is recommended that those chosen are validated for each different type of sample group. This study aimed to validate housekeeping genes for use in cryopreservation studies of zebrafish embryos. Seven potential housekeeping genes were analysed across fresh and chilled intact embryos and across fresh and frozen isolated blastomeres using the GeNorm and NormFinder software packages. Results suggest that combined use of β-actin and EF1α as housekeeping genes would be suitable for cryopreservation studies on zebrafish embryos and blastomeres. © 2009 Elsevier Inc. All rights reserved.

This data was imported from Web of Science (Lite):

Authors: Lin, C., Spikings, E., Zhang, T. and Rawson, D.

Journal: THERIOGENOLOGY

Volume: 71

Issue: 7

Pages: 1147-1155

eISSN: 1879-3231

ISSN: 0093-691X

DOI: 10.1016/j.theriogenology.2008.12.013

This data was imported from Europe PubMed Central:

Authors: Lin, C., Spikings, E., Zhang, T. and Rawson, D.

Journal: Theriogenology

Volume: 71

Issue: 7

Pages: 1147-1155

eISSN: 1879-3231

ISSN: 0093-691X

Cryopreservation success is usually analysed in terms of cell survival, although there are other potential effects that do not necessarily result in cell death. These include DNA damage, which could result in altered gene expression. Real-time reverse transcriptase PCR allows quantitative analysis of gene expression but usually requires analysis of a 'housekeeping' gene as an internal reference. As the stability of housekeeping genes varies significantly among different groups of samples, it is recommended that those chosen are validated for each different type of sample group. This study aimed to validate housekeeping genes for use in cryopreservation studies of zebrafish embryos. Seven potential housekeeping genes were analysed across fresh and chilled intact embryos and across fresh and frozen isolated blastomeres using the GeNorm and NormFinder software packages. Results suggest that combined use of beta-actin and EF1alpha as housekeeping genes would be suitable for cryopreservation studies on zebrafish embryos and blastomeres.

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