Professor Tiantian Zhang

01202 965721
tzhang at bournemouth dot ac dot uk
http://orcid.org/0000-0001-9332-2559

Emeritus Professor
Poole House P307, Talbot Campus, Fern Barrow, Poole, BH12 5BB

Biography
Research

Biography

Following her PhD research in Cryobiology, Professor Tiantian Zhang worked as research fellows before she was made Reader and Professor. She was appointed Director of Institute of Research in the Applied Natural Sciences at University of Bedfordshire in 2008 before joining Bournemouth University in 2012 as the Head of the Graduate School. She was also a member of the Executive Committee of the UK Council for Graduate Education. She is now the Deputy Dean of Research and Professional Practice in the Faculty of Science and Technology. Professor Tiantian Zhang is a leading figure internationally in research on cryopreservation of gametes and embryos of fish species and her research interests have been in the areas of cryopreservation of reproductive cells and the effect of cryopreservation on genome integrity and cellular metabolism with applications in biomedicine, conservation and aquaculture. Her research activity has led to over 150 publications and over 100 presentations at international conferences and workshops... Professor Tiantian Zhang has obtained substantial funding from funding bodies such as the UK Research Councils, Wellcome Trust and EU. She has supervised over 30 PhDs and other research degree students and has been an editor or referee of over ten scientific journals including the leading journals in the field of cryobiology such as Cryobiology and CryoLetters. She is a currently a member of the Board of Governors of the Society for Cryobiology and was the elected Chairman of the Society for Low Temperature Biology (SLTB) from 2005 to 2008 following serving as General Secretary, Treasurer and Committee Member of SLTB for 10 years. She received an award from Society of Cryobiology for her service in 2016.

Research

My current research interests and activities are in the areas of the cryopreservation of reproductive cells, tissues and embryos of aquatic species and studies on the effect of cryopreservation on genome and cellular metabolism of reproductive cells including stem cells. Although successful cryopreservation of sperm has been achieved with many mammalian species including humans, the successful cryopreservation of fish oocytes and embryos remained elusive. Successful cryopreservation of fish oocytes and embryos will have important applications in conservation of biodiversity, aquaculture and genomic studies, and research in this area remains an intellectual challenge. My research has contributed significantly to an understanding of the problems associated with fish oocyte and embryo cryopreservation, and will undoubtedly assist successful protocol design in the future. My earlier research also included studies on the effect of temperature on cytotoxicity in prokaryotic cells and ecotoxicological studies using fish cell lines and other biological materials.

Journal Articles

de Freitas, T.R., Marques, L.S., dos Santos, R.S., Dantas, R.V., Souza França, T., Rodrigues, R.B., Torres-Lozano, K.G., Albuquerque, E., Siqueira-Silva, D.H., Zhang, T., Streit, D.P., 2025. Gelatin capsules for post mortem vitrification of Piracanjuba (Brycon orbignyanus) ovarian tissue as an accessible solution for emergency genetic preservation. Animal Reproduction Science, 275.
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Rodrigues de Freitas, T., Giannotti Galuppo, A., Santos Marques, L., Batista Rodrigues, R., Perez Atehortúa, M., Souza França, T., dos Santos Teixeira, N., Valente dos Santos, W., Cossina Gomes, I., Sachett, A., Tercya, H., de Siqueira Silva, D.H., Gamba, D., Zhang, T., Streit, D.P., 2023. Hydrogel encapsulation as a handling and vitrification tool for zebrafish ovarian tissue. Theriogenology, 198, 153-163.
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Gallo, J.M., Rodrigues, R.B., Fornari, D.C., Povh, J.A., Ribeiro, R.P., Zhang, T., de Freitas, T.R., dos Santos Teixeira, N., Streit, D.P., 2022. Does semen quality of Colossoma macropomum change the productivity of larvae during the reproductive period? Aquaculture, 558.
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Sana, S., Hardouin, E.A., Paley, R., Zhang, T., Andreou, D., 2020. The complete mitochondrial genome of a parasite at the animal-fungal boundary. Parasites and Vectors, 13 (1).
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Marques, L.S., Fossati, A.A.N., Rodrigues, R.B., Da Rosa, H.T., Izaguirry, A.P., Ramalho, J.B., Moreira, J.C.F., Santos, F.W., Zhang, T., Streit, D.P., 2019. Slow freezing versus vitrification for the cryopreservation of zebrafish (Danio rerio) ovarian tissue. Scientific Reports, 9 (1).
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Anil, S., Rawson, D., Zhang, T., 2018. Development of molecular markers for zebrafish (Danio rerio) ovarian follicle growth assessment following in-vitro culture in cryopreservation studies. Cryobiology, 83, 75-83.
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Sana, S., Williams, C., Hardouin, E.A., Blake, A., Davison, P., Pegg, J., Paley, R., Zhang, T., Andreou, D., 2018. Phylogenetic and environmental DNA insights into emerging aquatic parasites: implications for risk management. International Journal for Parasitology, 48 (6), 473-481.
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Silva, L.A., Marques, L.S., Zhang, T., Rodrigues, R.B., Eloy, L.R., Streit, D.P., 2018. Production of live larvae following in vitro maturation of zebrafish oocytes. Theriogenology, 105, 115-119.
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Zhang, T., 2017. Importance of cryobanking in aquatic species conservation and aquaculture. Cryobiology.
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Sana, S., Hardouin, E.A., Gozlan, R.E., Ercan, D., Tarkan, A.S., Zhang, T., Andreou, D., 2017. Origin and invasion of the emerging infectious pathogen Sphaerothecum destruens. Emerging Microbes Infections, 6 (8), e76.
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Martínez-Páramo, S., Horváth, Á., Labbé, C., Zhang, T., Robles, V., Herráez, P., Suquet, M., Adams, S., Viveiros, A., Tiersch, T.R., Cabrita, E., 2017. Cryobanking of aquatic species. Aquaculture, 472, 156-177.
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Anil, S., Rawson, D.M., Zhang, T., 2016. In-vitro culture protocol development for early stage zebrafish ovarian follicles. Cryobiology, 73 (3), 433.
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Marques, L.S., Bos-Mikich, A., Godoy, L.C., Silva, L.A., Maschio, D., Zhang, T., Streit, D.P., 2015. Viability of zebrafish (Danio rerio) ovarian follicles after vitrification in a metal container. Cryobiology, 71 (3), 367-373.
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Desai, K., Spikings, E., Zhang, T., 2015. Use of methanol as cryoprotectant and its effect on sox genes and proteins in chilled zebrafish embryos. Cryobiology, 71 (1), 1-11.
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Chantzaropoulos, A., Nathanailides, C., Kokokiris, L., Barbouti, A., Zhang, T., 2015. A brief exposure to low pH prior to refrigerated storage reduces the motility and viability of goldfish sperm (Carassius auratus, Linnaeus, 1758). Journal of Applied Ichthyology, 31 (S1), 89-93.
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Desai, K., Spikings, E., Zhang, T., 2015. Short-term chilled storage of zebrafish (Danio rerio) embryos in cryoprotectant as an alternative to cryopreservation. Zebrafish, 12 (1), 111-120.
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Ahmed, R., Spikings, E., Zhou, S., Thompsett, A., Zhang, T., 2014. Pre-hybridisation: An efficient way of suppressing endogenous biotin-binding activity inherent to biotin-streptavidin detection system. Journal of Immunological Methods.
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Godoy, L.C., Streit, D.P., Zampolla, T., Bos-Mikich, A., Zhang, T., 2013. A study on the vitrification of stage III zebrafish (Danio rerio) ovarian follicles. Cryobiology, 67 (3), 347-354.
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Anil, S., Rawson, D.M., Zhang, T., Zhang, T., 2013. Development of cryopreservation protocol for zebrafish ovarian fragments using controlled slow cooling. Cryobiology, 67 (3), 433.
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Ahmed, R., Spikings, E., Thompsett, A., Zhou, S., Zhang, T., Zhang, T., 2013. Investigation of the effects of different cryopreservation parameters on the genome of zebrafish (Danio rerio) embryos. Cryobiology. Cryobiology, 67 (3), 433-434.
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Salavati, M., Ghafari, F., Zhang, T., Fouladi-Nashta, A.A., 2013. Influence of caffeine pretreatment on biphasic invitro maturation of dog oocytes. Theriogenology, 80 (7), 784-792.
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Godoy, L., Zampolla, T., Streit Jr, D., Bos-Mikich, A., Zhang, T., 2012. Vitrification of zebrafish (Danio rerio) ovarian follicles. Cryobiology, 65 (3), 344.
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Anil, S., Zampolla, T., Zhang, T., 2012. Development of molecular markers for stage I and stage II zebrafish ovarian follicles after in vitro culture. Cryobiology, 65 (3), 360.
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Salavati, M., Ghafari, F., Zhang, T., Fouladi-Nashta, A.A., 2012. Effects of oxygen concentration on in vitro maturation of canine oocytes in a chemically defined serum-free medium. Reproduction, 144 (5), 547-556.
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Wang, L., Zhou, G.B., Shi, W.Q., Shi, J.M., Tian, X.Z., Gao, C., Zhang, L., Zhu, S.E., Zhang, T.T., Zeng, S.M., Liu, G.S., 2012. First live offspring born in superovulated sika deer (Cervus nippon) after embryo vitrification. Theriogenology, 78 (7), 1627-1632.
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Spikings, E., Zampolla, T., Rawson, D., Wang, Y., Zhang, T., 2012. Effect of methanol on mitochondrial organization in zebrafish (Danio rerio) ovarian follicles. Theriogenology, 77 (1), 28-38.
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Zampolla, T., Rawson, D.M., Zhang, T., 2012. The use of fetal bovine serum for cryopreservation of stage III zebrafish (Danio rerio) ovarian follicles. Cryo Letters, 33 (6), 435-442.
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Nathanailides, C., Chanzaropoulos, T., Barbouti, A., Perdikaris, C., Zhang, T., 2011. DNA fragmentation, linear velocity and fertilising ability of reactivated cry op reserved goldfish sperm using different cryoprotectants. Biotechnology, 10 (6), 514-520.
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Zhang, T., 2011. Cryopreservation of fish gametes and its application in aquaculture and conservation. Cryobiology, 63 (3), 314.
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Anil, T., Zampolla, T., Zhang, T., 2011. Development of in vitro culture method for zebrafish ovarian tissute fragments. Cryobiology, 63 (3), 311.
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Desai, K., Spikings, E., Zhang, T., 2011. Studies on the role of hsp47 and hsp90 in zebrafish (Danio rerio) embryos under chilled conditions. Cryobiology, 63 (3), 334.
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Zampolla, T., Spikings, E., Rawson, D., Zhang, T., 2011. Cytoskeleton proteins F-actin and tubulin distribution and interaction with mitochondria in the granulosa cells surrounding stage III zebrafish (Danio rerio) oocytes. Theriogenology, 76 (6), 1110-1119.
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Desai, K., Spikings, E., Zhang, T., 2011. Effect of chilling on sox2, sox3 and sox19a gene expression in zebrafish (Danio rerio) embryos. Cryobiology, 63 (2), 96-103.
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Lin, C., Zhang, T., Kuo, F.W., Tsai, S., 2011. Gorgonian coral (Junceella juncea and Junceella fragilis) oocyte chilling sensitivity in the context of adenosine triphosphate response (ATP). Cryo Letters, 32 (2), 141-147.
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Zhang, T., 2011. Cryopreservation of fish gamates and it’s applications in aquaculture and conservation. Toxicology and Environmental Health Sciences, 3, S30.
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Zampolla, T., Spikings, E., Srirangarajah, S., Rawson, D.M., Zhang, T., 2011. Impact of cryoprotectants and cryopreservation on metabolic activity and cytoskeleton proteins of zebrafish (Danio rerio) ovarian fragments. Cryo Letters, 32 (6), 525-536.
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Anil, S., Ghafari, F., Zampolla, T., Rawson, D.M., Zhang, T., 2011. Studies on cryoprotectant toxicity to zebrafish (Danio rerio) ovarian tissue fragments. Cryo Letters, 32 (1), 40-50.
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Zampolla, T., Spikings, E., Zhang, T., Rawson, D., 2010. Investigation of methanol impact on mitochondrial transport and cytoskeleton proteins in stage III zebrafish (Danio rerio) ovarian follicles. Cryobiology, 61 (3), 385.
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Desai, K., Spikings, E., Rawson, D.M., Zhang, T., 2010. Studies on impact of chilling on sox gene expression in zebrafish (Danio rerio) embryos. Cryobiology, 61 (3), 389.
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Anil, S., Ghafari, F., Zampolla, T., Rawson, D.M., Zhang, T., 2010. Studies on cryoprotectant toxicity to zebrafish (Danio rerio) ovarian tissue fragments. Cryobiology, 61 (3), 384.
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Spikings, E., Vishnolia, K., Rawson, D.M., Zhang, T., 2010. Effect of methanol and chilling on expression of mitochondrial DNA replication factors and mitochondrial DNA replication in zebrafish embryos. Cryobiology, 61 (3), 391.
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Tsai, S., Rawson, D.M., Zhang, T., 2010. Development of in vitro culture method for early stage zebrafish (Danio rerio) ovarian follicles for use in cryopreservation studies. Theriogenology, 74 (2), 290-303.
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Rawson, D., Zhang, T., Zampolla, T., 2010. Cryo-banking of cells and tissues from freshwater and marine fish in the UK waters. Biopreservation and Biobanking, 7 (4), 185.
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Guan, M., Rawson, D.M., Zhang, T., 2010. Cryopreservation of zebrafish (Danio rerio) oocytes by vitrification. Cryo Letters, 31 (3), 230-238.
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Ghafari, F., Spikings, E., Rawson, D.M., Zhang, T., 2009. Effect of cryoprotectant treatment and chilling on oxidative stress in zebrafish (Danio rerio) early ovarian follicles. Cryobiology, 59 (3), 415.
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Zampolla, T., Spikings, E., Zhang, T., Rawson, D.M., 2009. Effect of methanol and DMSO exposure on mitochondrial activity and distribution in stage III ovarian follicles of zebrafish (Danio rerio). Cryobiology, 59 (3), 413.
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Zampolla, T., Spikings, E., Zhang, T., Rawson, D.M., 2009. Effect of methanol and Me2SO exposure on mitochondrial activity and distribution in stage III ovarian follicles of zebrafish (Danio rerio). Cryobiology, 59 (2), 188-194.
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Lin, C., Spikings, E., Zhang, T., Rawson, D.M., 2009. Effect of chilling and cryopreservation on expression of Pax genes in zebrafish (Danio rerio) embryos and blastomeres. Cryobiology, 59 (1), 42-47.
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Zampolla, T., Zhang, T., Holt, W.V., Rawson, D.M., 2009. Distributional arrangement of mitochondria in the granulosa cells surrounding stage III zebrafish (Danio rerio) oocytes. Theriogenology, 72 (1), 111-119.
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Tsai, S., Rawson, D.M., Zhang, T., 2009. Studies on chilling sensitivity of early stage zebrafish (Danio rerio) ovarian follicles. Cryobiology, 58 (3), 279-286.
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Tsai, S., Rawson, D.M., Zhang, T., 2009. Development of cryopreservation protocols for early stage zebrafish (Danio rerio) ovarian follicles using controlled slow cooling. Theriogenology, 71 (8), 1226-1233.
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Lin, C., Spikings, E., Zhang, T., Rawson, D., 2009. Housekeeping genes for cryopreservation studies on zebrafish embryos and blastomeres. Theriogenology, 71 (7), 1147-1155.
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Spikings, E., Zhang, T., Rawson, D., 2009. Effect of methanol on expression of mitochondrial DNA replication factors and mitochondrial DNA replication in zebrafish embryos. Cryoletters, 31 (2), 173-174.
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Tsai, S., Rawson, D., Zhang, T., 2009. Development of cryopreservation protocols for early stage zebrafish (Danio rerio) ovarian follicles using controlled slow cooling. Cryoletters, 30 (2), 162-163.
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Zampolla, T., Spikings, E., Zhang, T., Rawson, D., 2009. Effect of cryoprotectants exposure on mitochondrial activity and mtDNA copy number. Cryoletters, 30 (2), 160.
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Spikings, E., Zhang, T., Rawson, D., 2009. The effect of chilling on replication of mitochondrial DNA in zebrafish embryos. Cryoletters, 30 (2), 159.
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Lin, C., Spikings, E., Zhang, T., Rawson, D., 2009. Evaluation of potential housekeeping genes for use in expression profiling the effects of chilling and freezing in zebrafish embryos and blastomeres. Cryoletters, 30 (2), 157.
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Rawson, D.M., Zhang, T., 2009. Cryopreservation progress, challenges and applications in aquatic species. Cryoletters, 30 (1), 78.
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Lin, C., Zhang, T., Rawson, D.M., 2009. Cryopreservation of zebrafish (Danio rerio) blastomeres by controlled slow cooling. Cryo Letters, 30 (2), 132-141.
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Davies, J., Zhang, T., Fuller, B., Pillai, R., 2009. TO PERFORM A PILOT STUDY TO DEVELOP A QUALITY ASSESSMENT PROTOCOL FOR OPTIMISATION STUDIES AND FOR ROUTINE POST CRYOPRESERVATION USE IN CARDIOVASCULAR TISSUE BANKING. CRYOLETTERS, 30 (2), 153.
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Ding, Y., Zhang, T., Rawson, D.M., 2008. Mediated amperometric whole-cell biosensors for rapid in situ screening of heavy metal contaminated soil. Proceedings of 3rd International Conference on Soil Pollution and Remediation, 209-215.
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Chanzaropoulos, T., Nathanailides, C., Zhang, T., 2008. Computer assisted speam motility analysis (CASA) of goldfish after cryopreservation. Proceedings of 4th International Congress on Aquaculture, Fisheries Technology and Environmental Management.
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Babin, P.J., André, M., Forgue, J., Knoll-Gellida, A., Rawson, D.M., Zhang, T., 2008. Development of oocyte viability molecular signature (OVMS) assays for zebrafish (Danio rerio) oocyte cryopreservation studies. Cybium, 32 (2 SUPPL.), 217.
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Wang, R.Y., Guan, M., Rawson, D.M., Zhang, T., 2008. Ultrasound enhanced methanol penetration of zebrafish (Danio rerio) embryos measured by permittivity changes using impedance spectroscopy. Eur Biophys J, 37 (6), 1039-1044.
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Guan, M., Rawson, D.M., Zhang, T., 2008. Cryopreservation of zebrafish (Danio rerio) oocytes using improved controlled slow cooling protocols. Cryobiology, 56 (3), 204-208.
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Zhang, T., Rawson, D.M., Tosti, L., Carnevali, O., 2008. Cathepsin activities and membrane integrity of zebrafish (Danio rerio) oocytes after freezing to -196 degrees C using controlled slow cooling. Cryobiology, 56 (2), 138-143.
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Guan, M., Rawson, D.M., Zhang, T., 2008. Development of a new method for isolating zebrafish oocytes (Danio rerio) from ovary tissue masses. Theriogenology, 69 (3), 269-275.
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Lin, C.S., Zhang, T., Rawson, D.M., 2008. Cryopreservation and gene expression in zebrafish blastomeres. CryoLetters, 29 (1), 85.
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Babin, P.J., Andre, M., Forgue, J., Knoll-Gellida, A., Rawson, D.M., Zhang, T., 2008. Development of oocyte viability molecular signature (OVMS) assays for zebrafish (Danio rerio) oocyte cryopreservation studies. Cybium, 32 (2), 217.
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Zampolla, T., Zhang, T., Rawson, D.M., 2008. Evaluation of zebrafish (Danio rerio) ovarian follicle viability by simultaneous staining with fluorescein diacetate and propidium iodide. Cryo Letters, 29 (6), 463-475.
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Tsai, S., Rawson, D.M., Zhang, T., 2008. Studies on cryoprotectant toxicity to early stage zebrafish (Danio rerio) ovarian follicles. Cryo Letters, 29 (6), 477-483.
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Rawson, D.M., Zhang, T., 2008. The Frozen Ark project. CRYOLETTERS, 29 (1), 78.
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Tsai, S., Rawson, D.M., Zhang, T., 2008. Studies on cryoprotectant toxicity of early stage zebrafish (Danio rerio) oocytes. CRYOLETTERS, 29 (1), 84-85.
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Tsai, S., Rawson, D.M., Zhang, T., 2007. Studies on chilling sensitivity of early stage zebrafish (Danion rerio) oocytes. Cryobiology, 55, 364-365.
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Lin, C.S., Zhang, T., Elgar, G., Rawson, D.M., 2007. Initial studies on cryopreservation and gene expression in isolated blastomeres of zebrafish (Danio rerio). Cryobiology, 55, 365.
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Zhang, T., Rawson, D.M., 2007. Cryo-conservation in fish: the potentials and challenges. Cryobiology, 55, 354.
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Zhang, T., Rawson, D.M., 2007. The Frozen Ark: Cryo-banking of genetic and cellular material of endangered species. Cryobiology, 55, 355.
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Guan, M., Zhang, T., Rawson, D.M., 2006. Successful enzymatic separation of zebrafish (Danio rerio) oocytes using hyaluronidase. Cryobiology, 53, 418.
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Guan, T., Zhang, T., Rawson, D.M., 2006. Effects of improved controlled slow cooling protocols on the survival of zebrafish (Danio rerio) oocytes. Cryobiology, 53, 377-378.
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Zhang, T., Wang, R.Y., Bao, Q.-Y., Rawson, D.M., 2006. Development of a new rapid measurement technique for fish embryo membrane permeability studies using impedance spectroscopy. Theriogenology, 66 (4), 982-988.
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Wex, H., Rawson, D.M., Zhang, T., 2006. Use of biosensor and impedance spectroscopy assays to investigate the influence of temperature on E. coli sensitivity to 3,5-dichlorophenol. Electrochimica Acta, 51 (24), 5157-5162.
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Wang, R.Y., Zhang, T., Bao, Q., Rawson, D.M., 2006. Study on fish embryo responses to the treatment of cryoprotective chemicals using impedance spectroscopy. Eur Biophys J, 35 (3), 224-230.
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Kopeika, J., Zhang, T., Rawson, D., 2006. Zebrafish embryos (Danio rerio) using microinjection. Cryo Letters, 27 (5), 319-328.
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Zhang, T., Plachinta, M., Kopeika, J., Rawson, D.M., Cionna, C., Tosti, L., Carnevali, O., 2005. Membrane integrity and cathepsin activities of zebrafish (Danio rerio) oocytes after freezing to -196C using controlled slow cooling. Cryobiology, 51, 385-386.
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Rawson, D.M., Zhang, T., Wex, H., Bhatia, R., 2005. Cell based biosensors for environmental monitoring: challenges in exploiting cell bioassays in disposable sensor configurations. Cryobiology, 51, 384-385.
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Kopeika, J., Zhang, T., Rawson, D.M., 2005. Preliminary study on modification of yolk sec of zebrafish embryos (Danio rerio) using micro-injection. Cryobiology, 51, 405-406.
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Plachinta, M., Zhang, T., Rawson, D.M., 2005. Studies on the effect of certain supplements in cryoprotective medium on zebrafish (Danio rerio) oocytes quality after controlled slow cooling. Cryobiology, 51, 405.
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Adams, S.L., Zhang, T., Rawson, D.M., 2005. The effect of external medium composition on membrane water permeability of zebrafish (Danio rerio) embryos. Theriogenology, 64 (7), 1591-1602.
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118. Lubzens, E., Rosenfeld, H., Meiri, I., Olsson, P.E., Cerda, J., Babin, P.J., Admon, A., Carnevali, O., Rawson, D., Zhang, T., Dichtl, M., Pagelson, G., 2005. Cryopreservation of fish oocytes: Achievements and prospects. Proceddings of Larvi' 05- Fish &Shellfish Larviculture Symposium, 296-297.
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Zhang, T., Wex, H., Rawson, D.M., Thompson, R.S., 2005. Rapid ecotoxicological testing using transformed BF-2 cells incorporating a luminescent reporter gene. Toxicol In Vitro, 19 (6), 797-803.
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Zhang, T., Isayeva, A., Adams, S.L., Rawson, D.M., 2005. Studies on membrane permeability of zebrafish (Danio rerio) oocytes in the presence of different cryoprotectants. Cryobiology, 50 (3), 285-293.
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Kopeika, J., Zhang, T., Rawson, D.M., Elgar, G., 2005. Effect of cryopreservation on mitochondrial DNA of zebrafish (Danio rerio) blastomere cells. Mutation Research Fundamental and Molecular Mechanisms of Mutagenesis, 570 (1), 49-61.
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Kopeika, J., Zhang, T., Rawson, D.M., 2004. Cryoinjury to the genome. Any consequences? Cryobiology, 49, 322.
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Zhang, T., Bao, Q.-Y., Wang, Y., Rawson, D.M., 2004. Development of a new rapid measurement technique for fish embryo membrane permeability studies using impedance spectroscopy. Cryobiology, 49, 318.
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Plachinta, M., Zhang, T., Rawson, D.M., 2004. Preliminary studies on cryopreservation of zebrafish (Danio rerio) vitellogenic oocytes using controlled slow cooling. Cryobiology, 49, 347.
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Isayeva, A., Zhang, T., Rawson, D.M., 2004. Studies on membrane permeability of zebrafish (Danio rerio) oocytes in the presence of different cryoprotectants. Cryobiology, 49, 346-347.
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Adams, S.L., Zhang, T., Rawson, D.M., 2004. The effect of external medium composition on membrane water permeability of zebrafish (Danio rerio) embryos. Cryobiology, 49, 347-348.
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Daniel, M., Sharpe, A., Driver, J., Knight, A.W., Keenan, P.O., Walmsley, M.M., Robinson, A., Zhang, T., Rawson, D., 2004. Results of a technology demonstration project to compare rapid aquatic toxicity screening tests in the analysis of industrial effluents. Journal of Environmental Monitoring, 6 (11), 855-865.
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Isayeva, A., Zhang, T., Rawson, D.M., 2004. Studies on chilling sensitivity of zebrafish (Danio rerio) oocytes. Cryobiology, 49 (2), 114-122.
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Kopeika, J., Kopeika, E., Zhang, T., Rawson, D.M., Holt, W.V., 2004. Effect of DNA repair inhibitor (3-aminobenzamide) on genetic stability of loach (Misgurnus fossilis) embryos derived from cryopreserved sperm. Theriogenology, 61 (9), 1661-1673.
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Zhang, T., Robinson, A., Rawson, D.M., 2004. Biosensor applications in toxicity determination of chemical industrial effluents. Comparative Biochemistry and Physiology, 137 (3), 117.
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Plachinta, M., Zhang, T., Rawson, D.M., 2004. Studies on cryoprotectant toxicity to zebrafish (Danio rerio) oocytes. Cryo Letters, 25 (6), 415-424.
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Zhang, T., Plachinta, M., Isayeva, A., Rawson, D.M., 2003. Studies on zebrafish (Danio rerio) oocytes sensitivity to chilling and cryoprotectant toxicity. Cryobiology, 47, 269-270.
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Kopeika, J., Zhang, T., Rawson, D.M., Elgar, G., 2003. Effect of cryopreservation on mitochondrial DNA of zebrafish (Danio rerio) blastomere cells. Cryobiology, 47, 259.
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Kopeika, J., Kopeika, E., Zhang, T., Rawson, D.M., 2003. Studies on the toxicity of dimethyl sulfoxide, ethylene glycol, methanol and glycerol to loach (Misgurnus fossilis) sperm and the effect on subsequent embryo development. Cryo Letters, 24 (6), 365-374.
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Kopeika, J., Kopeika, E., Zhang, T., Rawson, D.M., Holt, W.V., 2003. Effect of DNA repair inhibitor (3-Aminobenzamide) on genetic stability of loach (Misgurnus fossilis) embryos derived from cryopreserved sperm. Theriogenology, 59 (7), 1545-1556.
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Kopeika, J., Kopeika, E., Zhang, T., Rawson, D.M., Holt, W.V., 2003. Effect of DNA repair inhibitor (3-Aminobenzamide) on genetic stability of loach (Misgurnus fossilis) embryos derived from cryopreserved sperm. Theriogenology, 59 (7), 1545-1556.
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Zhang, T., Liu, X.-H., Rawson, D.M., 2003. Effects of methanol and developmental arrest on chilling injury in zebrafish (Danio rerio) embryos. Theriogenology, 59 (7), 1545-1556.
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Kopeika, J., Kopeika, E., Zhang, T., Rawson, D.M., Holt, W.V., 2003. Detrimental effects of cryopreservation of loach (Misgurnus fossilis) sperm on subsequent embryo development are reversed by incubating fertilised eggs in caffeine. Cryobiology, 46 (1), 43-52.
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Kopeika, J., Kopeika, E., Zhang, T., Rawson, D.M., Holt, W.V., 2002. Detrimental effect of loach (Misgurnus fossilis) sperm cryopreservation on subsequent embryo development are reversed by incubating fertilised eggs in caffeine. Cryobiology, 46 (1), 43-52.
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Zhang, T., Rawson, D.M., 2002. Studies on cryopreservation of luc gene transfected bluegill sunfish fibroblast cell line. Cryo Letters, 23 (3), 191-196.
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Kopeika, J., Zhang, T., Kopeika, E., Holt, W.V., Rawson, D.M., 2001. Indirect evidence of genetic effect of cryopreservation on the sperm of loach (Missgurnus fossilis). Cryobiology, 43 (4), 388.
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Liu, X.H., Zhang, T., Rawson, D.M., 2001. Differential scanning calorimetry studies of intraembryonic freezing and cryoprotectant penetration in zebrafish (Danio rerio) embryos. J Exp Zool, 290 (3), 299-310.
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Liu, X.H., Zhang, T., Rawson, D.M., 2001. Effect of cooling rate and partial removal of yolk on the chilling injury in zebrafish (Danio rerio) embryos. Theriogenology, 55 (8), 1719-1731.
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Zhang, T., Bentley, A., Rawson, D.M., 2001. Cryopreservation of luc gene transfected bluegill sunfish fibroblast cell line. CryoLetters, 22 (2), 81-82.
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Liu, X.-H., Zhang, T., Rawson, D.M., 2000. Differential scanning calorimetry study on the characterisation of intraembryonic freezing and cryoprotectant penetration in zebrafish (Danio rerio) embryos. Cryobiology, 41 (4), 355-356.
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Rawson, D.M., Zhang, T., Kalicharan, D., Jongebloed, W.L., 2000. Field emission scanning electron microscopy and transmission electron microscopy studies of the chorion, plasma membrane and syncytial layers of the gastrula-stage embryo of the zebrafish Brachydanio rerio: A consideration of the structural and functional relationships with respect to cryoprotectant penetration. Aquaculture Research, 31 (3), 325-336.
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Rawson, D.M., Zhang, T., Kalicharan, D., Jongebloed, W.L., 2000. FE-SEM and TEM studies of the chorion, plasma membrane and syncytial layers of the gastrula stage embryo of the zebrafish (Brachydanio rerio): a consideration of the structural and functional relationship with respect to cryoprotectant penetration. Aquaculture Research, 31 (3), 325-336.
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Liu, X.-H., Zhang, T., Rawson, D.M., 1999. Effects of cooling rates and anoxia on the chilling sensitivity of zebrafish (Danio rerio) embryos. Cryobiology, 39 (4), 318-319.
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Liu, X.H., Zhang, T., Rawson, D.M., 1999. The effect of partial removal of yolk on the chilling sensitivity of zebrafish (Danio rerio) embryos. Cryobiology, 39 (3), 236-242.
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Liu, X.H., Zhang, T., Rawson, D.M., 1998. Feasibility of vitrification of zebrafish (Danio rerio) embryos using methanol. Cryo Letters, 19 (5), 309-318.
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Zhang, T., Rawson, D.M., 1998. Permeability of dechorionated one-cell and six-somite stage zebrafish (Brachydanio rerio) embryos to water and methanol. Cryobiology, 37 (1), 13-21.
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Stokroos, D., Kalicharan, D., Rawson, D.M., Zhang, T., 1998. Non coating and CRYO-FE-SEM of zebrafish (Brachydanio rerio) embryo blastodermal cells and syncytial layer. Proceedings of Dutch Society for Microscopy’98, Rynsbury.
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Zhang, T., Rawson, D.M., Jongebloed, W.L., 1998. Further studies on zebrafish (Brachydanio rerio) embryo membranes: permeability and ultra-structure. CryoLetters, 3, 65.
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Kalicharan, D., Jongebloed, W.L., Rawson, D.M., Zhang, T., 1998. Variations in fixation techniques for field emission SEM and TEM of zebrafish (Branchydanio rerio) embryo inner and outer membranes. Journal of Electron Microscopy, 47 (6), 645-658.
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Liu, X.-H., Zhang, T., Rawson, D.M., 1997. A preliminary study on vitrification of intact zebrafish (Brachydanio rerio) embryos using methanol. Cryobiology, 35 (4), 372.
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Jongebloed, W.L., Kalicharan, D., Zhang, T., Rawson, D.M., 1997. The zebrafish (Brachydanio rerio) egg/embryo inner and outer membrane: A FE-SEM and TEM investigation. Part 2: Fixation regimes. Proceedings of Dutch Society for Microscopy’97.
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Rawson, D.M., Zhang, T., Jongebloed, W.L., 1997. Structure-permeability relationships of the chorion and vitelline membranes of zebrafish (Brachydanio rerio) embryos. CryoLetters, 18 (2), 67.
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Zhang, T., Rawson, D.M., 1996. Permeability of the vitelline membrane of zebrafish (Brachydanio rerio) embryos to methanol and propane-1,2-diol. Cryo Letters, 17 (5), 273-280.
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Zhang, T., Maisse, G., Rawson, D.M., 1996. Preliminary studies on the cryopreservation of red blood cells of rainbow trout (Oncorhynchus mykiss) and European catfish (Silurus glanis L.). Cryo Letters, 17 (5), 303-308.
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Zhang, T., Rawson, D.M., 1996. Feasibility studies on vitrification of intact zebrafish (Brachydanio rerio) embryos. Cryobiology, 33 (1), 1-13.
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Zhang, T., Rawson, D.M., 1996. Permeability of the vitelline membrane of zebrafish (Brachydanio rerio) embryos to methanol and propane-1,2-diol. CryoLetters, 17 (6), 273-280.
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Zhang, T., Maisse, G., Rawson, D.M., 1996. Preliminary studies on the cryopreservation of red blood cells of rainbow trout (Oncorhynchus mykiss) and European catfish (Silurus glanis L.). CryoLetters, 17 (6), 303-308.
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Zhang, T., Rawson, D.M., 1995. Studies on chilling sensitivity of zebrafish (Brachydanio rerio) embryos. Cryobiology, 32 (3), 239-246.
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Zhang, T., Rawson, D.M., 1995. Preliminary studies on vitelline membrane permeability of zebrafish (Brachydanio rerio) embryos. CryoLetters, 16, 319.
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Ellis, J.B., Shutes, R.B., Revitt, D.M., Zhang, T.T., 1994. Use of macrophytes for pollution treatment in urban wetlands. Resources Conservation and Recycling, 11 (1-4), 1-12.
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Zhang, T., Rawson, D.M., 1993. Cryoprotectant toxicity and permeability studies on zebrafish (Brachydanio rerio) embryos. Cryobiology, 6, 640.
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Zhang, T., Rawson, D.M., Morris, G.J., 1993. Cryopreservation of pre-hatch embryos of zebrafish (Brachydanio rerio). Aquatic Living Resources, 6 (2), 145-153.
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Shutes, R.B.E., Ellis, J.B., Revitt, D.M., Zhang, T., 1993. The use of Typha latifolia for heavy metal pollution control in urban wetlands. Proceedings of International Symposium on Constructed Wetlands for Water Quality Improvement, 407-414.
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Zhang, T., 1993. The use of zebrafish (Brachydanio rerio) for toxicity testing. Local Economy, 3, 54-57.
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Zhang, T., Shutes, B., 1992. An uncommon taste for heavy metals. Industrial Waste Management, 10, 13.
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Zhang, T., Ellis, J.B., Revitt, D.M., Shutes, R.B.E., Xie, X.Y., 1990. The use of macrophytes for water pollution control in China and UK. IAWPRC Newsletter, 3, 25-31.
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Zhang, T., Ellis, J.B., Revitt, D.M., Shutes, R.B.E., 1990. Metal uptake and associated pollution control by Typha latifolia in urban wetlands. Proceedings of International Conference on the Use of Constructed Wetlands in Water Pollution Control, 451-459.
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Li, G., Zhang, T., 1986. Investigation of Gangutai conservation arae. Proceedings of National Conservation Symposium.
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Li, G., Zhang, T., 1986. Investigation of Gangutai conservation area. Proceedings of National Conservation Symposium (China), 67-75.
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Zhang, T., Wang, Y., 1985. The significance of Liaoning forest resources protection. Liaoning Economy (China), 3, 29-36.
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Xie, X., Zhang, Y.R., Zhang, T., 1984. Sewage purification by water hyacinth (Eichhornia crassipes). Environmental Science (China), 5, 45-56.
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Li, G., Zhang, T., 1984. Aspects of environmental pollution of Liaoning western dry area. Proceedings of Agricultural Ecology Society Symposium (China), 89-101.
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Li, G., Zhang, T., 1983. Effects of water pollution on Liaoning aquatic ecosystem. Proceedings of National Ecological Society Symposium (China), 75-92.
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Books

Streit, D.P., Zhang, T., Paredes, E., 2024. Cryobiology for South American Neotropical Fish Species.
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Chapters

Streit, D.P., Povh, J.A., Fornari, D.C., Benato, J.L., De Mello, F., Routledge, E.A.B., Paredes, E., Zhang, T., 2024. Potential Use of Germplasm Banks of Neotropical South American Species for Fish Farming in Brazil. Cryobiology for South American Neotropical Fish Species. 256-271.
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Streit, D.P., Rodrigues, R.B., Sanches, E.A., Povh, J.A., de Siqueira-Silva, D.H., Vasconcelos, A.C.N., Zhang, T., 2024. Sperm Cryopreservation Protocols for Neotropical South American Species. Cryobiology for South American Neotropical Fish Species. 193-219.
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Streit Jr, D.P., Godoy, L., Ribeiro, R.P., Fornari, D.C., Digmayer, M., Zhang, T., 2014. Cryopreservation of Embryos and Oocytes of South American Fish Species. In: Yamashiro, H., eds. Recent Advances in Cryopreservation. InTech.
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Zhang, T., Lubzens, E., 2008. Cryopreservation of fish oocytes. Methods in Reproductive Aquaculture: Marine and Freshwater Species. CRC Press.
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Kopeika, J., Kopeika, E., Zhang, T., Rawson, D.M., 2008. Cryopreservation of sperm of loach (Misgurnus fossilis). Methods in Reproductive Aquaculture: Marine and Freshwater Species. CRC Press.
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Zhang, T., Rawson, D.M., Pekarsky, I., Blais, I., Lubzens, E., 2007. Low temperature preservation of fish gonad cells and oocytes. 'The Fish Oocyte: From Basic Studies to Biotechnological Applications. Kluwer Academic Publishers B.V.
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Kopeika, E., Kopeika, J., Zhang, T., 2007. Cryopreservation of fish sperm. 203-217.
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Zhang, T., 2004. Cryopreservation of gametes and embryos of aquatic species. In: Fuller, B., Lane, N., Benson, E., eds. 'Life in the Frozen State. CRC Press.
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Zhang, T., 2001. Cryopreservation of fish eggs and embryos. In: Watson, P.F., Holt, W.V., eds. Wildlife Conservation for the Future. Taylor and Francis.
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Conferences

Anil, S., Rawson, D.M., Zhang, T., 2016. In-vitro culture protocol development for early stage zebrafish ovarian follicles. In: Cryo2016 53rd Annual Meeting of the Society for Cryobiology. 01/07/2016 Otawa, Canada.
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Zhang, T., 2015. Cryopreservation in Aquatic Species and Applications in Aquaculture and Conservation. In: International Workshop on Reproduction and Conservation in Fish 01/08/2015 Porto Alegre, Brazil.
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Zhang, T., 2014. Latest development on cryopreservation of reproductive materals of fish gamates. In: Workshop on Cryopreservation of Fish Gametes and Embryos 01/12/2014 Manaus, Brazil.
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Zhang, T., 2014. Cryopreservation of aquatic species. In: 50th Society for Low Temperature Biology Annual Conference 01/09/2014 London.
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Zhang, T., 2013. Cryopreservation of fishgGametes and applications in aquaculture, conservation and biomedicine. In: Workshop on the Biology of Fish Gametes 17/09/2013 Algarve, Portugal.
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Anil, S., Rawson, D.M., Zhang, T., 2013. Development of cryopreservation protocol for zebrafish ovarian fragments using controlled slow cooling. In: Cryo2013 50rd Annual Meeting of the Society for Cryobiology 01/07/2013 Bethesda, Maryland, USA.
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Ahmed, R., Spikings, E., Thompsett, A., Zhao, S., Zhang, T., 2013. Investigation of the effects of different cryopreservation parameters on the genome of zebrafish (Danio rerio) embryos. In: Cryo2013 50rd Annual Meeting of the Society for Cryobiology July 2013 01/07/2013 Bethesda, Maryland, USA.
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Ghaffarilaleh, V., Marei, W.F., Ghafari, F., Zhang, T., Fouladi-Nashta, A., 2012. Effects of different sizes of HA on development of sheep embryos and blastocyst survival after vitrification. In: Society for Reproduction and Fertility Conference. 01/07/2012 Edinburgh, UK.
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Godoy, L., Zampolla, T., Streit Jr, D., Bos-Mikich, A., Zhang, T., 2012. Vitrification of zebrafish (Danio rerio) ovarian follicles. In: Cryo2012 49th Annual Meeting of the Society for Cryobiology. 01/06/2012 Rosario, Argentina.
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Anil, S., Zampolla, T., Zhang, T., 2012. Development of molecular markers for stage I and stage II zebrafish ovarian follicles after in vitro culture. In: Cryo2012 49th Annual Meeting of the Society for Cryobiology. 01/06/2012 Rosario, Argentina.
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Anil, S., Zampolla, T., Zhang, T., 2012. Development of molecular markers for stage I and stage II zebrafish ovarian follicles after in vitro culture. In: Cryo2012 49th Annual Meeting of the Society for Cryobiology. 01/06/2012 Rosario, Argentina.
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Anil, S., Zampolla, T., Zhang, T., 2012. Development of molecular markers for stage I and stage II zebrafish ovarian follicles after in vitro culture. In: Cryo2012 49th Annual Meeting of the Society for Cryobiology 01/06/2012 Rosario Argentina.
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Zhou, S., Yan, H., Zhang, T., Beattie, J., 2012. An investigation into the optimum conditions for separating low abundance and abundant proteins in rat plasma using a seppro column. In: 2nd International Conference on Proteomic 2012 01/01/2012 Unknown.
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Salavati, M., Ghafari, F., Zhang, T., Fouladi-Nashta, A., 2012. Effects of oxygen concentration on in vitro maturation of canine oocytes. REPRODUCTION IN DOMESTIC ANIMALS, 47, 532.
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Zhang, T., 2011. Cryopreservation of fish gamates and its applications. In: 4th International Conference on Environmental Science 27/10/2011 Incheon, South Korea.
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Zhang, T., 2011. Cryopreservation of fish gametes and its applications. In: Society of Cryobiology Annual Conference Cryo2011 24/07/2011 Oregon, USA.
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Anil, S., Zampolla, T., Zhang, T., 2011. Development of in vitro culture method for zebrafish ovarian tissue fragments. In: Cryo2011 48th Annual Meeting of the Society for Cryobiology. 01/07/2011 Oregon, USA.
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Desai, K., Spikings, E., Zhang, T., 2011. Studies on the role of hsp47 and hsp90 in zebrafish (Danio rerio) embryos under chilled conditions. In: Cryo2011 48th Annual Meeting of the Society for Cryobiology. 01/07/2011 Oregon, USA.
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Desai, K., Spikings, E., Zhang, T., 2011. Effect of chilling on sox2, sox3 and sox9 gene expression in zebrafish (Danio rerio) embryos. In: Society of Low Temperature Biology Symposium 2011 ‘Regenerative Medichine and Cryobiology’. 01/03/2011 London UK.
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Zhang, T., 2011. Latest development of cryobiology research at LIRANS. In: 7. Workshop on ‘Latest Development on Cryopreservation of Fish Gametes and embryos’, Yellow Sea Fisheries Research Institute (YSFRI) 12/01/2011 Qingdao, China.
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Zhang, T., 2010. Cryopreservation of fish gametes – current state of the art. In: 8. Workshop on Application of Cryopreservation of Fish Gametes in Aquaculture 23/11/2010 State University of Maringa, Brazil.
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Zhang, T., 2010. Cryopreservation of fish gametes and it’s applications in aquaculture and conservation. In: 9. Workshop on Cryopreservation of Gametes and Embryos of Aquatic Species 11/11/2010 University of Rio Grande do Sul, Brazil.
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Zampolla, T., Spikings, E., Zhang, T., Rawson, D.M., 2010. Investigation of methanol impact on mitochondrial transport and cytoskeleton proteins in stage III zebrafish (Danio rerio) ovarian follicles. In: Cryo2010 47th Annual Meeting of the Society for Cryobiology. 01/07/2010 Bristol, UK.
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Zampolla, T., Zhang, T., Rawson, D.M., 2010. Cryoconservation and species diversity. In: The Fisheries Society of the British Isles Annual International Symposium ‘Fish and Climate Change’. 01/07/2010 Belfast, UK.
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Desai, K., Spikings, E., Rawson, D.M., Zhang, T., 2010. Studies on impact of chilling on sox gene expression in zebrafish (Danio rerio) embryos. In: Cryo2010 47th Annual Meeting of the Society for Cryobiology. 01/07/2010 Bristol, UK.
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Anil, S., Ghafari, F., Zampolla, T., Rawson, D.M., Zhang, T., 2010. Studies on cryoprotectant toxicity to zebrafish (Danio rerio) ovarian tissue fragments. In: Cryo2010 47th Annual Meeting of the Society for Cryobiology. 01/07/2010 Bristol, UK.
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Spikings, E., Vishnolia, K., Rawson, D.M., Zhang, T., 2010. Effect of methanol and chilling on expression of mitochondrial DNA replication factors and mitochondrial DNA replication in zebrafish embryos. In: Cryo2010 47th Annual Meeting of the Society for Cryobiology. 01/07/2010 Bristol, UK.
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Rawson, D.M., Zhang, T., Zampolla, T., 2010. Cryo-Banking of Cells and Tissues from Freshwater and Marine Fish in the UK Waters. In: Diversity in Biobanking: Embracing Differences, Harnessing Commonalities. Annual Meeting of International Society of Biological and Envarionmental Repositories. 01/05/2010 Rotterdam, Netherlands.
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Spikings, E., Zampolla, T., Wang, Y., Rawson, D.W., Zhang, T., 2010. Methanol disrupts mitochondrial organisation in zebrafish ovarian follicles. In: Controversies in Cryopreservation of Stem Cells, Reproductive Cells, Tissues and Organs. 01/04/2010 Valencia, Spain.
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Zhang, T., Zampolla, T., Srirangarajah, S., Ramesh, S., Rawson, D.M., 2010. Effect of cryoprotectants exposure and cryopreservation on zebrafish (Danio rerio) ovarian tissues. In: Controversies in Cryopreservation of Stem Cells, Reproductive Cells, Tissues and Organs. 01/04/2010 Valencia, Spain.
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Spikings, E., Zampolla, T., Wang, Y., Rawson, D.W., Zhang, T., 2010. Effect of cryoprotectant and chilling on oxidative stress in stage I zebrafish (Danio rerio) ovarian follicles. In: Controversies in Cryopreservation of Stem Cells, Reproductive Cells, Tissues and Organs. 01/04/2010 Valencia, Spain.
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Spikings, E., Zhang, T., Rawson, D.M., 2009. Effect of methanol on expression of mitochondrial DNA replication factors and mitochondrial DNA replication in zebrafish embryos. In: Society for Low Temperature Biology Scientific Meeting 01/09/2009 Hannover, Germany.
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Ghafari, F., Spikings, E., Rawson, D.M., Zhang, T., 2009. Effect of cryoprotectant treatment and chilling on oxidative stress in zebrafish (Danio rerio) early ovarian follicles. In: Cryo2009 46th Annual Meeting of the Society for Cryobiology. 01/07/2009 Sappora, Japan.
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Zampolla, T., Spikings, E., Zhang, T., Rawson, D.W., 2009. Effect of methanol and DMSO exposure on mitochondrial activity and distribution in stage III ovarian follicles of zebrafish (Danio rerio). In: Cryo2009 46th Annual Meeting of the Society for Cryobiology. 01/07/2009 Sappora, Japan.
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Zhang, T., Tsai, S., Rawson, D.M., 2008. Latest Development on Cryopreservation of Zebrafish (Danio rerio) Oocytes. In: The 10th World Conference on Animal Production. 01/11/2008 Cape Town, South Africa.
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Chanzaropoulos, T., Nathanailides, C., Zhang, T., 2008. Computer assisted speam motility analysis (CASA) of goldfish after cryopreservation. In: 4th International Congress on Aquaculture, Fisheries Technology and Environmental Management 01/11/2008 Athens, Greece.
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Ding, Y., Zhang, T., Rawson, D.M., 2008. Mediated amperometric whole-cell biosensors for rapid in situ screening of heavy metal contaminated soil. In: 3rd International Conference on Soil Pollution and Remediation 01/10/2008 Nanjing, China.
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Tsai, S., Rawson, D.M., Zhang, T., 2008. Development of cryopreservation protocols for early stage zebrafish (Danio rerio) ovarian follicles using controlled slow cooling. In: Society for Low Temerature Biology Scientific Meeting 01/09/2008 Copenhagen, Denmark.
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Zampolla, T., Spikings, E., Zhang, T., Rawson, D.M., 2008. Effect of cryoprotectants exposure on mitochondrial activity and mtDNA copy number. In: Society for Low Temerature Biology Scientific Meeting 01/09/2008 Copenhagen, Denmark.
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Spikings, E., Zhang, T., Rawson, D.M., 2008. The Effect of Chilling on Replication of Mitochondrial DNA in Zebrafish Embryos. In: Society for Low Temerature Biology Scientific Meeting 01/09/2008 Copenhagen, Denmark.
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Lin, C., Spikings, E., Zhang, T., Rawson, D.M., 2008. Evaluation of potential housekeeping genes for use in expression profiling the effects of chilling and freezing in zebrafish embryos and blastomeres. In: Society for Low Temerature Biology Scientific Meeting 01/09/2008 Copenhagen, Denmark.
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Rawson, D.M., Zhang, T., 2008. Cryopreservation of somatic cells, gametes and embryos of fish. In: BioCryoBanks meeting 17/06/2008 Trier, Germany.
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Spikings, E., Lin, C.-H., Zhang, T., Rawson, D.M., 2008. The effect of chilling on mitochondrial DNA replication in zebrafish embryos. In: 8th International Meeting on Zebrafish Development & Genetics. 01/06/2008 Madison, USA.
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Tsai, S., Rawson, D.M., Zhang, T., 2007. Studies on cryoprotectant toxicity of early stage zebrafish (Danio rerio). In: Society for Low Temperature Biology Annual Scientific Meeting. 01/09/2007 Derby, UK.
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Lin, C.-S., Zhang, T., Rawson, D.M., 2007. Cryopreservation and gene expression in zebrafish blastomeres. In: Society for Low Temperature Biology Annual Scientific Meeting. 01/09/2007 Derby.
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Zhang, T., 2007. Cryo-conservation in fish: the potentials and challenges. In: The 44th Annual Meeting of the Society for Cryobiology 29/07/2007 Lake Louise, Canada.
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Zhang, T., Rawson, D.M., 2007. Cryo-conservation in fish: the potentials and challenges. In: The 44th Annual Meeting of the Society for Crybiology 29/07/2007 Lake Louise, Canada.
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Tsai, S., Rawson, D.M., Zhang, T., 2007. Studies on chilling sensitivity of early stage zebrafish (Danion rerio) oocytes. In: The 44th Annual Meeting of the Society for Crybiology 29/07/2007 Lake Louise, Canada.
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Lin, C.-S., Zhang, T., Elgar, G., Rawson, D.M., 2007. Initial studies on cryopreservation and gene expression in isolated blastomeres of zebrafish (Danio rerio). In: The 44th Annual Meeting of the Society for Crybiology 29/07/2007 Lake Louise, Canada.
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Rawson, D.M., Zhang, T., 2007. The Frozen Ark: Cryo-banking of genetic and cellular material of endangered species. In: The 44th Annual Meeting of the Society for Crybiology 29/07/2007 Lake Louise, Canada.
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Lin, C., Zhang, T., Elgar, G., Rawson, D.M., 2007. Cryopreservation and gene expression in zebrafish blastomeres. In: The Zebrafish Genetics and Development Meeting 01/07/2007 Amsterdam, The Netherlands.
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Babin, P.J., Andre, M., Forgue, J., Knoll-Gellida, A., Rawson, D.M., Zhang, T., 2007. Development of oocyte viability molecular signature (OVMS) assays for zebrafish (Danio rerio) oocyte cryopreservation studies. In: 8th International Symposium on Reproductive Physiology of Fish (ISRPF 2007) 01/06/2007 St Malo, France.
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Lubzens, E., Rosenfeld, H., Meiri, I., Olsson, P.E., Cerd, J., Babin, P.J., Admon, A., Carnevali, O., Rawson, D.M., Zhang, T., 2007. A multidisciplinary approach for investigating the prospects for cryopreservation of fish oocytes. In: IMBC 2007 8th international marine biotechnology conference 01/03/2007 Eliat, Isreal.
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Zampolla, T., Rawson, D.M., Zhang, T., 2006. Development of new viability assessments methods for zebrafish (Danio rerio) oocytes. In: Cryo2006 01/07/2006 Hamburg, Germany.
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Guan, M., Zhang, T., Rawson, D.M., 2006. Successful enzymatic separation of zebrafish (Danio rerio) oocytes using hyaluronidase. In: Cryo2006 01/07/2006 Hamburg, Germany.
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Guan, M., Zhang, T., Rawson, D.M., 2006. Effects of improved controlled slow cooling protocols on the survival of zebrafish (Danio rerio) oocytes. In: Cryo2006 01/07/2006 Hamburg, Germany.
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Zhang, T., 2005. Latest development on cryopreservation of zebrafish (Danio rerio) oocytes. In: International Meeting on Oocytes Cryopreservation 01/09/2005 Bordeaux, France.
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Li, Y.-Z., Zhang, T., Wang, R.Y., Rawson, D.M., 2005. Effect of ultrasound treatment on survival of zebrafish (Danio rerio) oocytes and embryos. In: 41st Annual SLTB meeting 01/09/2005 York, UK.
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Lubzens, E., Rosenfeld, H., Meiri, I., Olsson, P.E., Cerda, J., Babin, P., Admon, A., Carnevali, O., Rawson, D.M., Zhang, T., Dichtl, M., Pagelson, G., 2005. Cryopreservation of fish oocytes: obstacles and achievements. In: 4th Fish & Shellfish Larviculture Symposium 01/09/2005 Belgium.
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Zhang, T., Plachinta, M., Kopeika, J., Rawson, D.M., Cionna, C., Tosti, L., Carnevali, O., 2005. Membrane integrity and cathepsin activities of zebrafish (Danio rerio) oocytes after freezing to -196C using controlled slow cooling. In: Annual Conference of the Society for Cryobiology (Cryo2005) 01/07/2005 Minneapolis USA.
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Rawson, D.M., Zhang, T., Wex, H., Bhatia, R., 2005. Cell based biosensors for environmental monitoring: challenges in exploiting cell bioassays in disposable sensor configurations. In: Annual Conference of the Society for Cryobiology (Cryo2005) 01/07/2005 Minneapolis USA.
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Zhang, T., 2005. Membrane integrity and cathepsin activities of zebrafish (Danio rerio) oocytes after freezing to -196C using controlled slow cooling. In: Annual Conference of the Society for Cryobiology (Cryo2005) 01/07/2005 Minneapolis, USA.
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Plachinta, M., Zhang, T., Rawson, D.M., 2005. Studies on the effect of certain supplements in cryoprotective medium on zebrafish (Danio rerio) oocytes quality after controlled slow cooling. In: Annual Conference of the Society for Cryobiology (Cryo2005) 01/07/2005 Minneapolis, USA.
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Kopeika, J., Zhang, T., Rawson, D.M., 2005. Preliminary study on modification of yolk sec of zebrafish embryos (Danio rerio) using micro-injection. In: Annual Conference of the Society for Cryobiology (Cryo2005) 01/07/2005 Minneeapolis, USA.
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Hannah, W., Zhang, T., Rawson, D.M., 2005. Use of biosensor and impedance spectroscopy assays to investigate the influence of temperature on E. coli sensitivity to 3,5-dichlorophenol. In: Bioelectrochemistry 2005 01/06/2005 Coimbra, Portugal.
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Rawson, D.M., Zhang, T., 2005. New approaches to the cryopreservation of fish oocytes and embryos. In: International Workshop on The Role of Biotechnology for the Characterisation and Conservation of Crop, Forestry, Animal and Fishery Genetic Resources 01/03/2005 Turin, Italy.
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Forgue, J., Knoll-Gellida, A., Andre, M., Zhang, T., Rawson, D.M., Babin, P.J., 2005. Transcriptome analysis of zebrafish oocyte and development of an optimized cryopreservation procedure. In: Annual INRA Scientific Meeting 01/03/2005 Tours, France.
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Zhang, T., 2004. Effect of ultrasound treatment on survival of zebrafish (Danio rerio) embryos. In: International Conferences on Preservation of Genetic Resources. 01/10/2004 St. Petersburg, Russia.
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Lubzens, E., Rosenfeild, H., Meiri, I., Olsson, P.-E., Cerda, J., Babin, P., Admon, A., Carnevali, O., Rawson, D.M., Zhang, T., Dichtl, M., Pagelson, G., 2004. Towards the development of technologies for cryopreservation of fish oocytes (CRYOCYTE). In: European Aquaculture Society Conference. 01/10/2004 Barcelona, Spain.
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Kopeika, J., Zhang, T., Rawson, D.M., 2004. Cryoinjury to the genome and its consequences. In: World Congress of Cryobiology and Cryomedicine (CRYO'2004) 01/07/2004 Beijing, China.
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Zhang, T., Bao, Q.-Y., Wang, R.Y., Rawson, D.M., 2004. Development of a new rapid measurement technique for fish embryo membrane permeability studies using impedance spectroscopy. In: World Congress of Cryobiology and Cryomedicine (CRYO'2004) 01/07/2004 Beijing, China.
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Plachinta, M., Zhang, T., Rawson, D.M., 2004. Preliminary studies on cryopreservation of zebrafish (Danio rerio) vitellogenic oocytes using controlled slow cooling. In: World Congress of Cryobiology and Cryomedicine (CRYO'2004) 01/07/2004 Beijing, China.
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Zhang, T., Rawson, D.M., 2004. Studies on membrane permeability of zebrafish (Danio rerio) oocytes in the presence of different cryoprotectants. In: World Congress of Cryobiology and Cryomedicine (CRYO'2004 01/07/2004 Beijing, China.
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Adams, S.L., Zhang, T., Rawson, D.M., 2004. The effect of external medium composition on membrane water permeability of zebrafish (Danio rerio) embryos. In: World Congress of Cryobiology and Cryomedicine (CRYO'2004). 01/07/2004 Beijing, China.
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Zhang, T., Rawson, D.M., 2004. Towards the development of technologies for cryopreservation of fish oocytes. In: EuroOcean 2004. European Conference on Marine Science and Ocean Technology. 01/05/2004 Gateway, Ireland.
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Zhang, T., 2004. Biosensor applications in toxicity determination of chemical industrial effluents. In: Royal Society of Chemistry, Society of the Chemical Industry, Society of Environmental Toxicology & Chemistry and the Society for Experimental Biology conference. 01/03/2004 Edinburgh, UK.
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Rawson, D.M., Zhang, T., 2003. The Challenges and potential of cryopreservation of fish eggs and embryos. In: International Marine Biotechnological Industry and Science Conference. 01/12/2003 Taiwan.
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Isayeva, A., Zhang, T., Rawson, D.M., 2003. Studies on toxicity of cryoprotectants to zebrafish (Danio rerio) oocytes. In: Society for Low Temperature Biology Annual Meeting. 02/09/2003 Potters Bar, UK.
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Zhang, T., Plachinta, M., Isayeva, A., Rawson, D.M., 2003. Studies on zebrafish (Danio rerio) oocytes sensitivity to chilling and cryoprotectant toxicity. In: CryoBioMol, 40th Annual Meeting of the Society for Cryobiology in conjunction with the Society of Low Temperature Biology. 01/09/2003 Coimbra, Portugal.
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Kopeika, J., Zhang, T., Rawson, D.M., Elgar, G., 2003. Effect of cryopreservation on mitochondrial DNA of zebrafish (Danio rerio) blastomere cells. In: CryoBioMol, 40th Annual Meeting of the Society for Cryobiology in conjunction with the Society of Low Temperature Biology. 01/09/2003 Coimbra, Portugal.
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Plachinta, M., Zhang, T., Rawson, D.M., 2003. Studies on chilling sensitivity to zebrafish (Danio rerio) oocytes. In: Society for Low Temperature Biology Annual Meeting. 01/09/2003 Potters Bar, UK.
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Wex, H., Zhang, T., Rawson, D.M., 2003. The influence of temperature on the sensitivity of microbes to toxicants. In: SETAC Europe 13th Annual Meeting 01/05/2003 Hamburg, Germany.
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Kopeika, J., Kopeika, E., Zhang, T., Rawson, D.M., Holt, W.V., 2002. Effect of inhibitor of the DNA repair system on the survival of embryos (Misgurnus fossilis), derived from cryopreserved sperm. In: International Institute of Refrigeration (IIR) International Conference in association with the Society of Low Temperature Biology 01/05/2002 Hradec Kralove, Czech Republic.
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Wex, H., Zhang, T., Depledge, M.H., Rawson, D.M., 2001. The effects of temperature on cytotoxicity in Escherichia coli. In: Society of Environmental Toxicology and Chemistry (UK) Annual Meeting 01/08/2001 University of East Anglia, UK.
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Kopeika, J., Zhang, T., Kopeika, E., Holt, W.V., Rawson, D.M., 2001. Indirect evidence of genetic effect of cryopreservation on the sperm of loach (Missgurnus fossilis). In: CRYO 2001, 38th meeting of the Society for Cryobiology in association with the Society for Low Temperature Biology 01/07/2001 Edinburgh, UK.
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Kopeika, J., Kopeika, E., Zhang, T., Rawson, D.M., 2001. Effect of caffeine on the survival of embryos (Misgurnus fossilis) derived from cryopreserved sperm. In: Society for Low Temperature Biology Annual Meeting. 01/04/2001 University of Luton, UK.
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Zhang, T., 2001. Problems associated with zebrafish (Danio rerio) embryo cryopreservation. In: 15. International Workshop on Conservation of Endangered Fish Species through the Cryopreservation of Fish Embryos. 01/03/2001 Washington DC, USA.
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Zhang, T., 2000. Factors limiting the successful cryopreservation of zebrafish (Danio rerio) embryos. In: 16. The First International Symposium on ‘Low Temperature Gene Bank for Aquaculture Rare and Endangered Fish and Other Hydrobionts Species’. 20/11/2000 Pushchino, Russia.
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Zhang, T., Bentley, A., Rawson, D.M., 2000. Cryopreservation of luc gene transfected bluegill sunfish fibroblast cell line. In: Society for Low Temperature Biology Symposium. 01/09/2000 Ambleside, UK.
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Liu, X.-H., Zhang, T., Rawson, D.M., 2000. Differential scanning calorimetry studies of intraembryonic freezing and cryoprotectant penetration in zebrafish (Danio rerio) embryos. In: CRYO 2000, 37th Annual Meeting of the Society for Cryobiology 01/08/2000 Boston, USA.
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Liu, X.-H., Zhang, T., Rawson, D.M., 1999. Effects of cooling rates and anoxia on the chilling sensitivity of zebrafish (Danio rerio) embryos. In: World Congress of Cryobiology (CRYO’99) 01/07/1999 Marseilles, France.
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Liu, X.-H., Zhang, T., Rawson, D.M., 1998. Effect of partial removal of yolk on the survival and chilling sensitivity of zebrafish (Danio rerio) embryos. In: Society for Low Temperature Biology Symposium. 01/09/1998 Bristol, UK.
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Zhang, T., Rawson, D.M., 1997. Further studies on zebrafish (Brachydanio rerio) embryo membranes: permeability and ultra-structure. In: Society for Low Temperature Biology Symposium: Cryopreservation and the Conservation of Biodiversity. 01/09/1997 London, UK.
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Liu, X.-H., Zhang, T., Rawson, D.M., 1997. A preliminary study on vitrification of intact zebrafish (Brachydanio rerio) embryos using methanol. In: CRYO 97, 34th Annual Conference of the Society for Cryobiology 01/06/1997 Barcelona, Spain.
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Rawson, D.M., Zhang, T., Jongebloed, W., 1996. Structure-permeability relationships of the chorion and vitelline membranes of zebrafish (Brachydanio rerio) embryos. In: Society for Low Temperature Biology Symposium. 01/09/1996 Dundee, UK.
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Zhang, T., Rawson, D.M., 1995. Preliminary studies on vitelline membrane permeability of zebrafish (Brachydanio rerio) embryos. In: Society for Low Temperature Biology Conference. 01/09/1995 Cardiff, UK.
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Zhang, T., Rawson, D.M., 1994. Studies on chilling sensitivity of zebrafish (Brachydanio rerio) embryos. In: Society for Low Temperature Biology Symposium - Cryopreservation of Oocytes. 01/11/1994 London, UK.
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Zhang, T., Rawson, D.M., 1993. Cryopreservation of zebrafish embryos by supercooling. In: Society for Low Temperature Biology Annual Symposium. 01/09/1993 Chelwood Gate, UK.
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Zhang, T., Rawson, D.M., 1993. Cryoprotectant toxicity and permeability studies on zebrafish (Brachydanio rerio) embryos. In: Society for Cryobiology 30th Annual Conference. 01/07/1993 Atlanta, USA.
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Zhang, T., Rawson, D.M., Morris, G.J., 1992. Cryopreservation of pre-hatch embryos of zebrafish (Brachydanio rerio). In: International Workshop on Gamete and Embryo Storage and Cryopreservation in Aquatic Organisms. 01/04/1992 Paris, France.
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Reports

Zhang, T., Plachinta, M., Rawson, D.M., 2005. Cryopreservation of zebrafish (Danio rerio) oocytes using controlled slow cooling and vitrification. EU FPV CRYOCYTE.
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Zhang, T., Isayeva, A., Plachinta, M., Rawson, D.M., 2004. Studies on zebrafish (Danio rerio) oocytes membrane permeability to water and cryoprotectants and their cryopreservation using controlled slow cooling. EU FPV CRYOCYTE.
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Zhang, T., Rawson, D.M., 2003. Technology demonstration project: rapid aquatic toxicity screening test methods. DTI/BIO-WISE, SOCSA project.
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Zhang, T., Isayeva, A., Plachinta, M., Rawson, D.M., 2003. Studies on zebrafish (Danio rerio) oocytes sensitivity to chilling and cryoprotectant toxicity. Research Report, EU FPV CRYOCYTE.
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Zhang, T., Rawson, D.M., 2001. Development of bioassays for ecotoxicological testing, based on transformed fish cell lines incorporating luminescent reporter genes. DTI/LINK cell engineering programme.
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Zhang, T., Li, G., 1987. Ecological evaluation of the impact of pesticides in Liaoning province. Liaoning Environmental Protection Institute, China.
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Zhang, T., 1985. Environmental impact of Liaoning nuclear power plant. Liaoning Environmental Protection Institute, China.
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Grants

Grants

Development of cryopreservation protocols for gametes and embryos of migratory Brazilian fish (Brazilian Federal Agency for the Support & Evaluation of Graduate Education, Brazil, 01 Sep 2013). Awarded
Cryopreservation of oocytes of endangered species by vitrification (Science and Technology Council of HE, Ministry of Education, Brazil, 01 Jan 2011). Awarded
PhD Studentship (HEFCE ORSAS, 01 Sep 2009). Awarded
DNA Damage, Repair & Cryopreservation (HEFCE Research Informed Teaching Grant, 01 Sep 2008). Awarded
Cryopreservation of fish sperm (Greek Ministry of Education, 01 Sep 2005). Awarded
Investigation of new approaches to the cryopreservation of fish embryos (The Wellcome Trust Functional Genomics Development Initiative (GR06988MA), 01 Sep 2003). Awarded
Towards the development of technologies for cryopreservation of fish gametes (European Commission, Quality of Life and Management of Living Resources, 01 Sep 2002). Awarded
Cryopreservation of fish eggs and embryos (HEFCE, 01 Sep 1996). Awarded
Bioassays for ecotoxicological testing, based on transformed fish cell lines (DTI/LINK Cell Engineering Programme, 01 Sep 1992). Awarded

External Responsibilities

External Responsibilities

Society of Cryobiology, Member of Board of Governors (2013-)
Chinese Life Science Society UK, Vice President (2011-)
China Agriculture University, Visiting Professor (2008-)
BBSRC, USA, Grant Referee (2007-)
Society for Low Temperature Biology, Chairman
Cyro Letters, Associate Editor
Journal of Aquaculture R&D, Board Member

Qualifications

Qualifications

PhD in Cryobiology (University of Bedfordshire, 1994)
MPhil in Environmental Biology (Middlesex University, 1990)
BSc (Hons) in Environmental Biology (Liaoning University (China), 1982)

The data on this page was last updated at 22:00 on February 11, 2026.

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