Ultrasound enhanced methanol penetration of zebrafish (Danio rerio) embryos measured by permittivity changes using impedance spectroscopy

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This data was imported from PubMed:

Authors: Wang, R.Y., Guan, M., Rawson, D.M. and Zhang, T.

Journal: Eur Biophys J

Volume: 37

Issue: 6

Pages: 1039-1044

ISSN: 0175-7571

DOI: 10.1007/s00249-007-0229-0

Studies on permittivity changes in fish embryos measured by impedance spectroscopy after ultrasound treatment during exposure to cryoprotectant is reported here for the first time. The permittivity changes of zebrafish embryos in cryoprotectant solutions before and after ultrasound treatment were measured using impedance spectroscopy. Zebrafish (Danio rerio) embryos at 50% epiboly stage were exposed to 2 M methanol for 25 min before ultrasound treatment for 5 min at 22 degrees C. Embryos were treated with ultrasound in different frequencies (24 and 48 kHz) and voltages (50, 100, 150 and 175 V) combinations. The results showed a clear increasing trend of permittivity from voltage 50 to 175 V over lower impedance frequency range of 10-10(3) Hz indicating increased methanol penetration into the embryos after ultrasound treatment. The embryo survival was not compromised after ultrasound treatment under conditions used in the present study. The use of impedance spectroscopy technique provides a useful none-invasive tool for detecting changes of cryoprotectant penetration in fish embryos after ultrasound treatment. The technique is especially useful for the selection of the suitable cryoprotectants in embryo cryopreservation and may also allow quantitative measurements in embryo membrane permeability studies.

This data was imported from Scopus:

Authors: Wang, R.Y., Guan, M., Rawson, D.M. and Zhang, T.

Journal: European Biophysics Journal

Volume: 37

Issue: 6

Pages: 1039-1044

ISSN: 0175-7571

DOI: 10.1007/s00249-007-0229-0

Studies on permittivity changes in fish embryos measured by impedance spectroscopy after ultrasound treatment during exposure to cryoprotectant is reported here for the first time. The permittivity changes of zebrafish embryos in cryoprotectant solutions before and after ultrasound treatment were measured using impedance spectroscopy. Zebrafish (Danio rerio) embryos at 50% epiboly stage were exposed to 2 M methanol for 25 min before ultrasound treatment for 5 min at 22°C. Embryos were treated with ultrasound in different frequencies (24 and 48 kHz) and voltages (50, 100, 150 and 175 V) combinations. The results showed a clear increasing trend of permittivity from voltage 50 to 175 V over lower impedance frequency range of 10-103 Hz indicating increased methanol penetration into the embryos after ultrasound treatment. The embryo survival was not compromised after ultrasound treatment under conditions used in the present study. The use of impedance spectroscopy technique provides a useful none-invasive tool for detecting changes of cryoprotectant penetration in fish embryos after ultrasound treatment. The technique is especially useful for the selection of the suitable cryoprotectants in embryo cryopreservation and may also allow quantitative measurements in embryo membrane permeability studies. © 2007 EBSA.

This data was imported from Web of Science (Lite):

Authors: Wang, R.Y., Guan, M., Rawson, D.M. and Zhang, T.

Journal: EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS

Volume: 37

Issue: 6

Pages: 1039-1044

ISSN: 0175-7571

DOI: 10.1007/s00249-007-0229-0

This data was imported from Europe PubMed Central:

Authors: Wang, R.Y., Guan, M., Rawson, D.M. and Zhang, T.

Journal: European biophysics journal : EBJ

Volume: 37

Issue: 6

Pages: 1039-1044

eISSN: 1432-1017

ISSN: 0175-7571

Studies on permittivity changes in fish embryos measured by impedance spectroscopy after ultrasound treatment during exposure to cryoprotectant is reported here for the first time. The permittivity changes of zebrafish embryos in cryoprotectant solutions before and after ultrasound treatment were measured using impedance spectroscopy. Zebrafish (Danio rerio) embryos at 50% epiboly stage were exposed to 2 M methanol for 25 min before ultrasound treatment for 5 min at 22 degrees C. Embryos were treated with ultrasound in different frequencies (24 and 48 kHz) and voltages (50, 100, 150 and 175 V) combinations. The results showed a clear increasing trend of permittivity from voltage 50 to 175 V over lower impedance frequency range of 10-10(3) Hz indicating increased methanol penetration into the embryos after ultrasound treatment. The embryo survival was not compromised after ultrasound treatment under conditions used in the present study. The use of impedance spectroscopy technique provides a useful none-invasive tool for detecting changes of cryoprotectant penetration in fish embryos after ultrasound treatment. The technique is especially useful for the selection of the suitable cryoprotectants in embryo cryopreservation and may also allow quantitative measurements in embryo membrane permeability studies.

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