The effect of partial removal of yolk on the chilling sensitivity of zebrafish (Danio rerio) embryos

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This data was imported from PubMed:

Authors: Liu, X.H., Zhang, T. and Rawson, D.M.

Journal: Cryobiology

Volume: 39

Issue: 3

Pages: 236-242

eISSN: 1090-2392

DOI: 10.1006/cryo.1999.2206

The effect of partial removal of yolk on the survival of zebrafish embryos and the chilling sensitivity of yolk-reduced embryos were investigated at several stages of embryo development. Dechorionated embryos were punctured with a sharp microneedle and approximately 50 to 75% of yolk content was released following multiple punctures. The survival of yolk-reduced embryos was found to be stage dependent. Only 7.9% of 26-somite (24 h) embryos survived, whereas 56.7% of prim-6 (27 h), 62.4% of prim-15 (34 h), and 81.3% of high-pec (49 h) embryos survived after partial removal of yolk. For chilling sensitivity studies the yolk-reduced embryos at high-pec stage were cultured in embryo medium for 2, 6, or 24 h to allow embryo recovery before they were chilled at 0 degrees C for 6 h. No significant differences (P > 0.05) were seen in normalized survivals between control and yolk-reduced embryos following a 2- or 6-h recovery period. However, when the recovery period was extended to 24 h, the yolk-reduced embryos showed significant (P < 0.05) higher survival than that of chilled controls and the significance was more pronounced (P < 0.01) after a longer period (10 h) of chilling. Similar results were also obtained with embryos at prim-6 stage. These results indicated that after partial removal of yolk, zebrafish embryos at post-prim-6 stage can survive well and their sensitivity to chilling can be reduced. This may have significant implications in alleviating certain difficulties confronting the cryopreservation of fish embryos. Copyright 1999 Academic Press.

This data was imported from Scopus:

Authors: Liu, X.H., Zhang, T. and Rawson, D.M.

Journal: Cryobiology

Volume: 39

Issue: 3

Pages: 236-242

ISSN: 0011-2240

DOI: 10.1006/cryo.1999.2206

The effect of partial removal of yolk on the survival of zebrafish embryos and the chilling sensitivity of yolk-reduced embryos were investigated at several stages of embryo development. Dechorionated embryos were punctured with a sharp microneedle and approximately 50 to 75% of yolk content was released following multiple punctures. The survival of yolk- reduced embryos was found to he stage dependent. Only 7.9% of 26-somite (24 h) embryos survived, whereas 56.7% of prim-6 (27 h), 62.4% of prim-15 (34 h), and 81.3% of high-pec (49 h) embryos survived after partial removal of yolk. For chilling sensitivity studies the yolk-reduced embryos at high-pec stage were cultured in embryo medium for 2, 6, or 24 h to allow embryo recovery before they were chilled at 0°C for 6 h. No significant differences (P > 0.05) were seen in normalized survivals between control and yolk-reduced embryos following a 2- or 6-h recovery period. However, when the recovery period was extended to 24 h, the yolk-reduced embryos showed significant (P < 0.05) higher survival than that of chilled controls and the significance was more pronounced (P < 0.01) after a longer period (10 h) of chilling. Similar results were also obtained with embryos at prim-6 stage. These results indicated that after partial removal of yolk, zebrafish embryos at post-prim- 6 stage can survive well and their sensitivity to chilling can he reduced. This may have significant implications in alleviating certain difficulties confronting the cryopreservation of fish embryos.

This data was imported from Europe PubMed Central:

Authors: Liu, X.H., Zhang, T. and Rawson, D.M.

Journal: Cryobiology

Volume: 39

Issue: 3

Pages: 236-242

eISSN: 1090-2392

ISSN: 0011-2240

The effect of partial removal of yolk on the survival of zebrafish embryos and the chilling sensitivity of yolk-reduced embryos were investigated at several stages of embryo development. Dechorionated embryos were punctured with a sharp microneedle and approximately 50 to 75% of yolk content was released following multiple punctures. The survival of yolk-reduced embryos was found to be stage dependent. Only 7.9% of 26-somite (24 h) embryos survived, whereas 56.7% of prim-6 (27 h), 62.4% of prim-15 (34 h), and 81.3% of high-pec (49 h) embryos survived after partial removal of yolk. For chilling sensitivity studies the yolk-reduced embryos at high-pec stage were cultured in embryo medium for 2, 6, or 24 h to allow embryo recovery before they were chilled at 0 degrees C for 6 h. No significant differences (P > 0.05) were seen in normalized survivals between control and yolk-reduced embryos following a 2- or 6-h recovery period. However, when the recovery period was extended to 24 h, the yolk-reduced embryos showed significant (P < 0.05) higher survival than that of chilled controls and the significance was more pronounced (P < 0.01) after a longer period (10 h) of chilling. Similar results were also obtained with embryos at prim-6 stage. These results indicated that after partial removal of yolk, zebrafish embryos at post-prim-6 stage can survive well and their sensitivity to chilling can be reduced. This may have significant implications in alleviating certain difficulties confronting the cryopreservation of fish embryos. Copyright 1999 Academic Press.

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