First live offspring born in superovulated sika deer (Cervus nippon) after embryo vitrification
Authors: Wang, L., Zhou, G.B., Shi, W.Q., Shi, J.M., Tian, X.Z., Gao, C., Zhang, L., Zhu, S.E., Zhang, T.T., Zeng, S.M., Liu, G.S.
Journal: Theriogenology
Publication Date: 15/10/2012
Volume: 78
Issue: 7
Pages: 1627-1632
ISSN: 0093-691X
DOI: 10.1016/j.theriogenology.2012.07.008
Abstract:The rapid growth in sika deer (Cervus nippon) farming and interest in their conservation is an impetus for development of embryo transfer (ET) procedures. However, a paucity of research has prevented widespread application of ET in this species. The objective of the present study was to establish a multiple ovulation and ET procedure with both fresh and vitrified embryos in sika deer. Multiparous weaned hinds (N = 18) were used as embryo donors during the reproductive season of 2008 at a local breeding farm in China. Estrus was synchronized in donors and recipients (N = 38) by inserting a controlled internal drug release for 12 days (insertion = Day 0). Superovulation was induced with a total of 320 mg of NIH-FSH-P1 (Folltropin-V; Bioniche, Belleville, ON, Canada) given as 40 mg im every 12 h from the afternoon of Day 9 to the morning of Day 13. After estrus was detected, donors were artificially inseminated using a transcervical technique. The embryo recovery rate was 76.8% (63/82), including 1.6% (1/63), 77.8% (49/63), and 1.6% (1/63) blastocysts, morula, and eight-cell embryos, respectively. After transfer of fresh and vitrified embryos, pregnancy rates were 85.7% and 61.6% and birth rates were 64.3% and 53.9% (P > 0.05). In conclusion, we developed a satisfactory multiple ovulation and ET procedure in farmed sika deer using vitrified embryos. © 2012 Elsevier Inc.
Source: Scopus
Preferred by: Tiantian Zhang
First live offspring born in superovulated sika deer (Cervus nippon) after embryo vitrification.
Authors: Wang, L., Zhou, G.B., Shi, W.Q., Shi, J.M., Tian, X.Z., Gao, C., Zhang, L., Zhu, S.E., Zhang, T.T., Zeng, S.M., Liu, G.S.
Journal: Theriogenology
Publication Date: 15/10/2012
Volume: 78
Issue: 7
Pages: 1627-1632
eISSN: 1879-3231
DOI: 10.1016/j.theriogenology.2012.07.008
Abstract:The rapid growth in sika deer (Cervus nippon) farming and interest in their conservation is an impetus for development of embryo transfer (ET) procedures. However, a paucity of research has prevented widespread application of ET in this species. The objective of the present study was to establish a multiple ovulation and ET procedure with both fresh and vitrified embryos in sika deer. Multiparous weaned hinds (N = 18) were used as embryo donors during the reproductive season of 2008 at a local breeding farm in China. Estrus was synchronized in donors and recipients (N = 38) by inserting a controlled internal drug release for 12 days (insertion = Day 0). Superovulation was induced with a total of 320 mg of NIH-FSH-P1 (Folltropin-V; Bioniche, Belleville, ON, Canada) given as 40 mg im every 12 h from the afternoon of Day 9 to the morning of Day 13. After estrus was detected, donors were artificially inseminated using a transcervical technique. The embryo recovery rate was 76.8% (63/82), including 1.6% (1/63), 77.8% (49/63), and 1.6% (1/63) blastocysts, morula, and eight-cell embryos, respectively. After transfer of fresh and vitrified embryos, pregnancy rates were 85.7% and 61.6% and birth rates were 64.3% and 53.9% (P > 0.05). In conclusion, we developed a satisfactory multiple ovulation and ET procedure in farmed sika deer using vitrified embryos.
Source: PubMed
First live offspring born in superovulated sika deer (<i>Cervus nippon</i>) after embryo vitrification
Authors: Wang, L., Zhou, G.B., Shi, W.Q., Shi, J.M., Tian, X.Z., Gao, C., Zhang, L., Zhu, S.E., Zhang, T.T., Zeng, S.M., Liu, G.S.
Journal: THERIOGENOLOGY
Publication Date: 2012
Volume: 78
Issue: 7
Pages: 1627-1632
ISSN: 0093-691X
DOI: 10.1016/j.theriogenology.2012.07.008
Source: Web of Science
First live offspring born in superovulated sika deer (Cervus nippon) after embryo vitrification.
Authors: Wang, L., Zhou, G.B., Shi, W.Q., Shi, J.M., Tian, X.Z., Gao, C., Zhang, L., Zhu, S.E., Zhang, T.T., Zeng, S.M., Liu, G.S.
Journal: Theriogenology
Publication Date: 10/2012
Volume: 78
Issue: 7
Pages: 1627-1632
eISSN: 1879-3231
ISSN: 0093-691X
DOI: 10.1016/j.theriogenology.2012.07.008
Abstract:The rapid growth in sika deer (Cervus nippon) farming and interest in their conservation is an impetus for development of embryo transfer (ET) procedures. However, a paucity of research has prevented widespread application of ET in this species. The objective of the present study was to establish a multiple ovulation and ET procedure with both fresh and vitrified embryos in sika deer. Multiparous weaned hinds (N = 18) were used as embryo donors during the reproductive season of 2008 at a local breeding farm in China. Estrus was synchronized in donors and recipients (N = 38) by inserting a controlled internal drug release for 12 days (insertion = Day 0). Superovulation was induced with a total of 320 mg of NIH-FSH-P1 (Folltropin-V; Bioniche, Belleville, ON, Canada) given as 40 mg im every 12 h from the afternoon of Day 9 to the morning of Day 13. After estrus was detected, donors were artificially inseminated using a transcervical technique. The embryo recovery rate was 76.8% (63/82), including 1.6% (1/63), 77.8% (49/63), and 1.6% (1/63) blastocysts, morula, and eight-cell embryos, respectively. After transfer of fresh and vitrified embryos, pregnancy rates were 85.7% and 61.6% and birth rates were 64.3% and 53.9% (P > 0.05). In conclusion, we developed a satisfactory multiple ovulation and ET procedure in farmed sika deer using vitrified embryos.
Source: Europe PubMed Central