Gorgonian coral (Junceella juncea and Junceella fragilis) oocyte chilling sensitivity in the context of adenosine triphosphate response (ATP)

This data was imported from PubMed:

Authors: Lin, C., Zhang, T., Kuo, F.W. and Tsai, S.

Journal: Cryo Letters

Volume: 32

Issue: 2

Pages: 141-147

ISSN: 0143-2044

Gorgonian corals are suffering continued decline in population and reproductive ability because of environmental changes. Cryopreservation can play an important role in ex situ conservation for these corals. In the present study, oocyte chilling sensitivity in the context of adenosine triphosphate (ATP) response in two gorgonian species (Junceella juncea and Junceella fragilis) and the effectiveness of cryoprotectants in protecting coral oocytes from chilling injury were studied in an attempt to develop protocols for their cryopreservation. Oocytes of two gorgonian corals were exposed to methanol (1 M, 2 M) and EG (1 M) at 5, 0 and -5 degree C for up to 216 hours, and ATP levels in oocytes were then determined. ATP levels decreased gradually with exposure time and 1M methanol was more effective in protecting oocytes from chilling injury than other cryoprotectant treatments tested. J. juncea oocytes were less sensitive to chilling than J. fragilis oocytes. This study provided useful information for development of cryopreservtion protocols for the two gorgonian coral oocytes.

This source preferred by Tiantian Zhang

This data was imported from Scopus:

Authors: Lin, C., Zhang, T., Kuo, F.W. and Tsai, S.

Journal: Cryo-Letters

Volume: 32

Issue: 2

Pages: 141-147

ISSN: 0143-2044

Gorgonian corals are suffering continued decline in population and reproductive ability because of environmental changes. Cryopreservation can play an important role in 'ex situ' conservation for these corals.the present study, oocyte chilling sensitivity in the context of adenosine triphosphate (ATP) response in two gorgonian species (Junceella juncea and Junceella fragilis) and the effectiveness of cryoprotectants in protecting coral oocytes from chilling injury were studied in an attempt to develop protocols for their cryopreservation. Oocytes of two gorgonian corals were exposed to methanol (1 M, 2 M) and EG (1 M) at 5, 0 and -5°C for up to 216 hours, and ATP levels in oocytes were then determined. ATP levels decreased gradually with exposure time and IM methanol was more effective in protecting oocytes from chilling injury than other cryoprotectant treatments tested. J. juncea oocytes were less sensitive to chilling than J. fragilis oocytes. This study provided useful information for development of cryopreservtion protocols for the two gorgonian coral oocytes. © CryoLetters.

This data was imported from Web of Science (Lite):

Authors: Lin, C., Zhang, T., Kuo, F.W. and Tsai, S.

Journal: CRYOLETTERS

Volume: 32

Issue: 2

Pages: 141-147

eISSN: 1742-0644

ISSN: 0143-2044

This data was imported from Europe PubMed Central:

Authors: Lin, C., Zhang, T., Kuo, F.W. and Tsai, S.

Journal: Cryo letters

Volume: 32

Issue: 2

Pages: 141-147

ISSN: 0143-2044

Gorgonian corals are suffering continued decline in population and reproductive ability because of environmental changes. Cryopreservation can play an important role in ex situ conservation for these corals. In the present study, oocyte chilling sensitivity in the context of adenosine triphosphate (ATP) response in two gorgonian species (Junceella juncea and Junceella fragilis) and the effectiveness of cryoprotectants in protecting coral oocytes from chilling injury were studied in an attempt to develop protocols for their cryopreservation. Oocytes of two gorgonian corals were exposed to methanol (1 M, 2 M) and EG (1 M) at 5, 0 and -5 degree C for up to 216 hours, and ATP levels in oocytes were then determined. ATP levels decreased gradually with exposure time and 1M methanol was more effective in protecting oocytes from chilling injury than other cryoprotectant treatments tested. J. juncea oocytes were less sensitive to chilling than J. fragilis oocytes. This study provided useful information for development of cryopreservtion protocols for the two gorgonian coral oocytes.

The data on this page was last updated at 05:17 on May 25, 2020.