Iodomethane production by two important marine cyanobacteria: Prochlorococcus marinus (CCMP 2389) and Synechococcus sp. (CCMP 2370)

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Authors: Hughes, C., Franklin, D.J. and Malin, G.

Journal: Marine Chemistry

Volume: 125

Pages: 19-25

ISSN: 0304-4203

DOI: 10.1016/j.marchem.2011.01.00

Iodomethane (CH3I) production by Prochlorococcus marinus (CCMP 2389) and Synechococcus sp. (CCMP 2370) was investigated using laboratory cultures. In contrast to Synechococcus, where no trace gas production was observed, P. marinus cells produced CH3I. The rate of CH3I production by P. marinus was found to vary depending on the physiological state of the cells as defined by photosynthetic health (Fv/Fm) measurements, SYTOX Green staining (which evaluates membrane permeability) and calculated in vivo fluorescence per cell (a proxy of pigment content per cell). The highest total cell normalised production rates (7189 [range 5225–11,517] molecules CH3I cellTotal day−1) were observed when Fv/Fm was ≤ 0.3 and ≥ 75% of the cells were SYTOX Green labelled. Relatively lower rates of CH3I production (146 [103–267] molecules CH3I cellTotal day− 1) were observed when Fv/Fm was ≥ 0.5 and ≤ 20% of the P. marinus cells were SYTOX Green labelled. Our results suggest that discrepancies in the CH3I production rates by Prochlorococcus observed between previous studies could be due to differing cell physiology. Calculations are presented which suggest that Prochlorococcus could contribute significantly to CH3I production in the eastern tropical Atlantic Ocean. This study highlights the importance of considering cell physiological state when determining trace gas production rates in laboratory culture and extrapolating them to the natural environment.

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Authors: Hughes, C., Franklin, D.J. and Malin, G.

Journal: Marine Chemistry

Volume: 125

Issue: 1-4

Pages: 19-25

ISSN: 0304-4203

DOI: 10.1016/j.marchem.2011.01.007

Iodomethane (CH 3 I) production by Prochlorococcus marinus (CCMP 2389) and Synechococcus sp. (CCMP 2370) was investigated using laboratory cultures. In contrast to Synechococcus, where no trace gas production was observed, P. marinus cells produced CH 3 I. The rate of CH 3 I production by P. marinus was found to vary depending on the physiological state of the cells as defined by photosynthetic health (F v /F m ) measurements, SYTOX Green staining (which evaluates membrane permeability) and calculated in vivo fluorescence per cell (a proxy of pigment content per cell). The highest total cell normalised production rates (7189 [range 5225-11,517] molecules CH 3 I cell Total day -1 ) were observed when F v /F m was ≤0.3 and ≥75% of the cells were SYTOX Green labelled. Relatively lower rates of CH 3 I production (146 [103-267] molecules CH 3 I cell Total day -1 ) were observed when F v /F m was ≥0.5 and ≤20% of the P. marinus cells were SYTOX Green labelled. Our results suggest that discrepancies in the CH 3 I production rates by Prochlorococcus observed between previous studies could be due to differing cell physiology. Calculations are presented which suggest that Prochlorococcus could contribute significantly to CH 3 I production in the eastern tropical Atlantic Ocean. This study highlights the importance of considering cell physiological state when determining trace gas production rates in laboratory culture and extrapolating them to the natural environment. © 2011 Elsevier B.V.

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