Protection of cells from salinity stress by extracellular polymeric substances in diatom biofilms
Authors: Steele, D.J., Franklin, D.J. and Underwood, G.J.C.
Journal: Biofouling
Volume: 30
Issue: 8
Pages: 987-998
eISSN: 1029-2454
ISSN: 0892-7014
DOI: 10.1080/08927014.2014.960859
Abstract:Diatom biofilms are abundant in the marine environment. It is assumed (but untested) that extracellular polymeric substances (EPS), produced by diatoms, enable cells to cope with fluctuating salinity. To determine the protective role of EPS, Cylindrotheca closterium was grown in xanthan gum at salinities of 35, 50, 70 and 90 ppt. A xanthan matrix significantly increased cell viability (determined by SYTOX-Green), growth rate and population density by up to 300, 2,300 and 200%, respectively. Diatoms grown in 0.75% w/v xanthan, subjected to acute salinity shock treatments (at salinities 17.5, 50, 70 and 90 ppt) maintained photosynthetic capacity, Fq′/Fm′, within 4% of pre-shock values, whereas Fq′/Fm′ in cells grown without xanthan declined by up to 64% with hypersaline shock. Biofilms that developed in xanthan at standard salinity helped cells to maintain function during salinity shock. These results provide evidence of the benefits of living in an EPS matrix for biofilm diatoms.
https://eprints.bournemouth.ac.uk/23344/
Source: Scopus
Preferred by: Daniel Franklin
Protection of cells from salinity stress by extracellular polymeric substances in diatom biofilms.
Authors: Steele, D.J., Franklin, D.J. and Underwood, G.J.C.
Journal: Biofouling
Volume: 30
Issue: 8
Pages: 987-998
eISSN: 1029-2454
DOI: 10.1080/08927014.2014.960859
Abstract:Diatom biofilms are abundant in the marine environment. It is assumed (but untested) that extracellular polymeric substances (EPS), produced by diatoms, enable cells to cope with fluctuating salinity. To determine the protective role of EPS, Cylindrotheca closterium was grown in xanthan gum at salinities of 35, 50, 70 and 90 ppt. A xanthan matrix significantly increased cell viability (determined by SYTOX-Green), growth rate and population density by up to 300, 2,300 and 200%, respectively. Diatoms grown in 0.75% w/v xanthan, subjected to acute salinity shock treatments (at salinities 17.5, 50, 70 and 90 ppt) maintained photosynthetic capacity, Fq'/Fm', within 4% of pre-shock values, whereas Fq'/Fm' in cells grown without xanthan declined by up to 64% with hypersaline shock. Biofilms that developed in xanthan at standard salinity helped cells to maintain function during salinity shock. These results provide evidence of the benefits of living in an EPS matrix for biofilm diatoms.
https://eprints.bournemouth.ac.uk/23344/
Source: PubMed
Protection of cells from salinity stress by extracellular polymeric substances in diatom biofilms
Authors: Steele, D.J., Franklin, D.J. and Underwood, G.J.C.
Journal: BIOFOULING
Volume: 30
Issue: 8
Pages: 987-998
eISSN: 1029-2454
ISSN: 0892-7014
DOI: 10.1080/08927014.2014.960859
https://eprints.bournemouth.ac.uk/23344/
Source: Web of Science (Lite)
Protection of cells from salinity stress by extracellular polymeric substances in diatom biofilms.
Authors: Steele, D.J., Franklin, D.J. and Underwood, G.J.C.
Journal: Biofouling
Volume: 30
Issue: 8
Pages: 987-998
eISSN: 1029-2454
ISSN: 0892-7014
DOI: 10.1080/08927014.2014.960859
Abstract:Diatom biofilms are abundant in the marine environment. It is assumed (but untested) that extracellular polymeric substances (EPS), produced by diatoms, enable cells to cope with fluctuating salinity. To determine the protective role of EPS, Cylindrotheca closterium was grown in xanthan gum at salinities of 35, 50, 70 and 90 ppt. A xanthan matrix significantly increased cell viability (determined by SYTOX-Green), growth rate and population density by up to 300, 2,300 and 200%, respectively. Diatoms grown in 0.75% w/v xanthan, subjected to acute salinity shock treatments (at salinities 17.5, 50, 70 and 90 ppt) maintained photosynthetic capacity, Fq'/Fm', within 4% of pre-shock values, whereas Fq'/Fm' in cells grown without xanthan declined by up to 64% with hypersaline shock. Biofilms that developed in xanthan at standard salinity helped cells to maintain function during salinity shock. These results provide evidence of the benefits of living in an EPS matrix for biofilm diatoms.
https://eprints.bournemouth.ac.uk/23344/
Source: Europe PubMed Central
Protection of cells from salinity stress by extracellular polymeric substances in diatom biofilms.
Authors: Steele, D.J., Franklin, D.J. and Underwood, G.
Journal: Biofouling
Volume: 30
Issue: 8
Pages: 987-998
ISSN: 0892-7014
Abstract:Diatom biofilms are abundant in the marine environment. It is assumed (but untested) that extracellular polymeric substances (EPS), produced by diatoms, enable cells to cope with fluctuating salinity. To determine the protective role of EPS, Cylindrotheca closterium was grown in xanthan gum at salinities of 35, 50, 70 and 90 ppt. A xanthan matrix significantly increased cell viability (determined by SYTOX-Green), growth rate and population density by up to 300, 2,300 and 200%, respectively. Diatoms grown in 0.75% w/v xanthan, subjected to acute salinity shock treatments (at salinities 17.5, 50, 70 and 90 ppt) maintained photosynthetic capacity, Fq'/Fm', within 4% of pre-shock values, whereas Fq'/Fm' in cells grown without xanthan declined by up to 64% with hypersaline shock. Biofilms that developed in xanthan at standard salinity helped cells to maintain function during salinity shock. These results provide evidence of the benefits of living in an EPS matrix for biofilm diatoms.
https://eprints.bournemouth.ac.uk/23344/
http://www.tandfonline.com/toc/gbif20/30/8
Source: BURO EPrints