A brief exposure to low pH prior to refrigerated storage reduces the motility and viability of goldfish sperm (Carassius auratus, Linnaeus, 1758)
Authors: Chantzaropoulos, A., Nathanailides, C., Kokokiris, L., Barbouti, A. and Zhang, T.
Journal: Journal of Applied Ichthyology
Volume: 31
Issue: S1
Pages: 89-93
eISSN: 1439-0426
ISSN: 0175-8659
DOI: 10.1111/jai.12735
Abstract:Optimal conditions of short-term storage of fish sperm are important for successful artificial reproduction protocols or incubation of sperm in a cryoprotecting medium for successful sperm cryopreservation. The influence of acid or alkaline incubating conditions on short term storage of goldfish sperm was investigated in a series of experiments. Goldfish sperm was diluted an immobilizing solution (1: 3) and incubated for 1 h in acidic, pH 6.5 (AC group) or alkaline, pH 8.5 (AL group) conditions. Subsequently, the sperm of both groups was further diluted (1: 3) in the immobilizing solution and the pH was adjusted to pH 7.51 (±0.02) and 7.56 (±0.07) in the AC and AL group respectively. The samples were kept in sealed bags and stored at 4°C. Viability (%) and motility (%) was estimated in samples obtained daily for the next 4 days. The results indicate that even a brief exposure of sperm to acid acidic conditions prior to storage accelerated the decline of sperm viability and motility during refrigeration.
https://eprints.bournemouth.ac.uk/25008/
Source: Scopus
A brief exposure to low pH prior to refrigerated storage reduces the motility and viability of goldfish sperm (<i>Carassius auratus</i>, Linnaeus, 1758)
Authors: Chantzaropoulos, A., Nathanailides, C., Kokokiris, L., Barbouti, A. and Zhang, T.
Journal: JOURNAL OF APPLIED ICHTHYOLOGY
Volume: 31
Pages: 89-93
eISSN: 1439-0426
ISSN: 0175-8659
DOI: 10.1111/jai.12735
https://eprints.bournemouth.ac.uk/25008/
Source: Web of Science (Lite)
A brief exposure to low pH prior to refrigerated storage reduces the motility and viability of goldfish sperm (Carassius auratus, Linnaeus, 1758)
Authors: Chantzaropoulos, A., Nathanailides, C., Kokokiris, L., Barbouti, A. and Zhang, T.
Journal: Journal of Applied Ichthyology
Volume: 31
Issue: S1
Pages: 89-93
ISSN: 0175-8659
Abstract:Optimal conditions of short-term storage of fish sperm are important for successful artificial reproduction protocols or incubation of sperm in a cryoprotecting medium for successful sperm cryopreservation. The influence of acid or alkaline incubating conditions on short term storage of goldfish sperm was investigated in a series of experiments. Goldfish sperm was diluted an immobilizing solution (1: 3) and incubated for 1 h in acidic, pH 6.5 (AC group) or alkaline, pH 8.5 (AL group) conditions. Subsequently, the sperm of both groups was further diluted (1: 3) in the immobilizing solution and the pH was adjusted to pH 7.51 (±0.02) and 7.56 (±0.07) in the AC and AL group respectively. The samples were kept in sealed bags and stored at 4°C. Viability (%) and motility (%) was estimated in samples obtained daily for the next 4 days. The results indicate that even a brief exposure of sperm to acid acidic conditions prior to storage accelerated the decline of sperm viability and motility during refrigeration.
https://eprints.bournemouth.ac.uk/25008/
Source: BURO EPrints