Laboratory and field validation of a simple method for detecting four species of non-native freshwater fish using eDNA

This data was imported from PubMed:

Authors: Davison, P.I., Créach, V., Liang, W.-J., Andreou, D., Britton, J.R. and Copp, G.H.

http://eprints.bournemouth.ac.uk/24595/

Journal: J Fish Biol

Volume: 89

Issue: 3

Pages: 1782-1793

eISSN: 1095-8649

DOI: 10.1111/jfb.13086

This paper presents the first phase in the development and validation of a simple and reliable environmental (e)DNA method using conventional PCR to detect four species of non-native freshwater fish: pumpkinseed Lepomis gibbosus, sunbleak Leucaspius delineatus, fathead minnow Pimephales promelas and topmouth gudgeon Pseudorasbora parva. The efficacy of the approach was demonstrated in indoor tank (44 l) trials in which all four species were detected within 24 h. Validation was through two field trials, in which L. gibbosus was detected 6-12 h after its introduction into outdoor experimental ponds and P. parva was successfully detected in disused fish rearing ponds where the species was known to exist. Thus, the filtration of small (30 ml) volumes of pond water was sufficient to capture fish eDNA and the approach emphasised the importance of taking multiple water samples of sufficient spatial coverage for detecting species of random or patchy distribution.

This data was imported from Scopus:

Authors: Davison, P.I., Créach, V., Liang, W.J., Andreou, D., Britton, J.R. and Copp, G.H.

http://eprints.bournemouth.ac.uk/24595/

Journal: Journal of Fish Biology

Volume: 89

Issue: 3

Pages: 1782-1793

eISSN: 1095-8649

ISSN: 0022-1112

DOI: 10.1111/jfb.13086

© 2016 Crown copyright. Journal of Fish Biology © 2016 The Fisheries Society of the British Isles. This paper presents the first phase in the development and validation of a simple and reliable environmental (e)DNA method using conventional PCR to detect four species of non-native freshwater fish: pumpkinseed Lepomis gibbosus, sunbleak Leucaspius delineatus, fathead minnow Pimephales promelas and topmouth gudgeon Pseudorasbora parva. The efficacy of the approach was demonstrated in indoor tank (44 l) trials in which all four species were detected within 24 h. Validation was through two field trials, in which L. gibbosus was detected 6-12 h after its introduction into outdoor experimental ponds and P. parva was successfully detected in disused fish rearing ponds where the species was known to exist. Thus, the filtration of small (30 ml) volumes of pond water was sufficient to capture fish eDNA and the approach emphasised the importance of taking multiple water samples of sufficient spatial coverage for detecting species of random or patchy distribution.

This data was imported from Scopus:

Authors: Davison, P.I., Créach, V., Liang, W.J., Andreou, D., Britton, J.R. and Copp, G.H.

http://eprints.bournemouth.ac.uk/24595/

Journal: Journal of Fish Biology

Volume: 89

Issue: 3

Pages: 1782-1793

eISSN: 1095-8649

ISSN: 0022-1112

DOI: 10.1111/jfb.13086

© 2016 Crown copyright. Journal of Fish Biology © 2016 The Fisheries Society of the British Isles This paper presents the first phase in the development and validation of a simple and reliable environmental (e)DNA method using conventional PCR to detect four species of non-native freshwater fish: pumpkinseed Lepomis gibbosus, sunbleak Leucaspius delineatus, fathead minnow Pimephales promelas and topmouth gudgeon Pseudorasbora parva. The efficacy of the approach was demonstrated in indoor tank (44 l) trials in which all four species were detected within 24 h. Validation was through two field trials, in which L. gibbosus was detected 6–12 h after its introduction into outdoor experimental ponds and P. parva was successfully detected in disused fish rearing ponds where the species was known to exist. Thus, the filtration of small (30 ml) volumes of pond water was sufficient to capture fish eDNA and the approach emphasised the importance of taking multiple water samples of sufficient spatial coverage for detecting species of random or patchy distribution.

This data was imported from Scopus:

Authors: Davison, P.I., Créach, V., Liang, W.J., Andreou, D., Britton, J.R. and Copp, G.H.

http://eprints.bournemouth.ac.uk/24595/

Journal: Journal of Fish Biology

eISSN: 1095-8649

ISSN: 0022-1112

DOI: 10.1111/jfb.13086

© 2016 The Fisheries Society of the British Isles. This paper presents the first phase in the development and validation of a simple and reliable environmental (e)DNA method using conventional PCR to detect four species of non-native freshwater fish: pumpkinseed Lepomis gibbosus, sunbleak Leucaspius delineatus, fathead minnow Pimephales promelas and topmouth gudgeon Pseudorasbora parva. The efficacy of the approach was demonstrated in indoor tank (44 l) trials in which all four species were detected within 24 h. Validation was through two field trials, in which L. gibbosus was detected 6-12 h after its introduction into outdoor experimental ponds and P. parva was successfully detected in disused fish rearing ponds where the species was known to exist. Thus, the filtration of small (30 ml) volumes of pond water was sufficient to capture fish eDNA and the approach emphasised the importance of taking multiple water samples of sufficient spatial coverage for detecting species of random or patchy distribution.

This data was imported from Web of Science (Lite):

Authors: Davison, P.I., Creach, V., Liang, W.-J., Andreou, D., Britton, J.R. and Copp, G.H.

http://eprints.bournemouth.ac.uk/24595/

Journal: JOURNAL OF FISH BIOLOGY

Volume: 89

Issue: 3

Pages: 1782-1793

eISSN: 1095-8649

ISSN: 0022-1112

DOI: 10.1111/jfb.13086

This data was imported from Europe PubMed Central:

Authors: Davison, P.I., Créach, V., Liang, W.J., Andreou, D., Britton, J.R. and Copp, G.H.

http://eprints.bournemouth.ac.uk/24595/

Journal: Journal of fish biology

Volume: 89

Issue: 3

Pages: 1782-1793

eISSN: 1095-8649

ISSN: 0022-1112

This paper presents the first phase in the development and validation of a simple and reliable environmental (e)DNA method using conventional PCR to detect four species of non-native freshwater fish: pumpkinseed Lepomis gibbosus, sunbleak Leucaspius delineatus, fathead minnow Pimephales promelas and topmouth gudgeon Pseudorasbora parva. The efficacy of the approach was demonstrated in indoor tank (44 l) trials in which all four species were detected within 24 h. Validation was through two field trials, in which L. gibbosus was detected 6-12 h after its introduction into outdoor experimental ponds and P. parva was successfully detected in disused fish rearing ponds where the species was known to exist. Thus, the filtration of small (30 ml) volumes of pond water was sufficient to capture fish eDNA and the approach emphasised the importance of taking multiple water samples of sufficient spatial coverage for detecting species of random or patchy distribution.

The data on this page was last updated at 04:55 on June 17, 2019.