Laboratory and field validation of a simple method for detecting four species of non-native freshwater fish using eDNA
Authors: Davison, P.I., Créach, V., Liang, W.J., Andreou, D., Britton, J.R. and Copp, G.H.
Journal: Journal of Fish Biology
eISSN: 1095-8649
ISSN: 0022-1112
DOI: 10.1111/jfb.13086
Abstract:© 2016 The Fisheries Society of the British Isles. This paper presents the first phase in the development and validation of a simple and reliable environmental (e)DNA method using conventional PCR to detect four species of non-native freshwater fish: pumpkinseed Lepomis gibbosus, sunbleak Leucaspius delineatus, fathead minnow Pimephales promelas and topmouth gudgeon Pseudorasbora parva. The efficacy of the approach was demonstrated in indoor tank (44 l) trials in which all four species were detected within 24 h. Validation was through two field trials, in which L. gibbosus was detected 6-12 h after its introduction into outdoor experimental ponds and P. parva was successfully detected in disused fish rearing ponds where the species was known to exist. Thus, the filtration of small (30 ml) volumes of pond water was sufficient to capture fish eDNA and the approach emphasised the importance of taking multiple water samples of sufficient spatial coverage for detecting species of random or patchy distribution.
Source: Scopus
Laboratory and field validation of a simple method for detecting four species of non-native freshwater fish using eDNA
Authors: Davison, P.I., Créach, V., Liang, W.J., Andreou, D., Britton, J.R. and Copp, G.H.
Journal: Journal of Fish Biology
Volume: 89
Issue: 3
Pages: 1782-1793
eISSN: 1095-8649
ISSN: 0022-1112
DOI: 10.1111/jfb.13086
Abstract:© 2016 Crown copyright. Journal of Fish Biology © 2016 The Fisheries Society of the British Isles This paper presents the first phase in the development and validation of a simple and reliable environmental (e)DNA method using conventional PCR to detect four species of non-native freshwater fish: pumpkinseed Lepomis gibbosus, sunbleak Leucaspius delineatus, fathead minnow Pimephales promelas and topmouth gudgeon Pseudorasbora parva. The efficacy of the approach was demonstrated in indoor tank (44 l) trials in which all four species were detected within 24 h. Validation was through two field trials, in which L. gibbosus was detected 6–12 h after its introduction into outdoor experimental ponds and P. parva was successfully detected in disused fish rearing ponds where the species was known to exist. Thus, the filtration of small (30 ml) volumes of pond water was sufficient to capture fish eDNA and the approach emphasised the importance of taking multiple water samples of sufficient spatial coverage for detecting species of random or patchy distribution.
Source: Scopus
Laboratory and field validation of a simple method for detecting four species of non-native freshwater fish using eDNA
Authors: Davison, P.I., Créach, V., Liang, W.J., Andreou, D., Britton, J.R. and Copp, G.H.
Journal: Journal of Fish Biology
Volume: 89
Issue: 3
Pages: 1782-1793
eISSN: 1095-8649
ISSN: 0022-1112
DOI: 10.1111/jfb.13086
Abstract:This paper presents the first phase in the development and validation of a simple and reliable environmental (e)DNA method using conventional PCR to detect four species of non-native freshwater fish: pumpkinseed Lepomis gibbosus, sunbleak Leucaspius delineatus, fathead minnow Pimephales promelas and topmouth gudgeon Pseudorasbora parva. The efficacy of the approach was demonstrated in indoor tank (44 l) trials in which all four species were detected within 24 h. Validation was through two field trials, in which L. gibbosus was detected 6-12 h after its introduction into outdoor experimental ponds and P. parva was successfully detected in disused fish rearing ponds where the species was known to exist. Thus, the filtration of small (30 ml) volumes of pond water was sufficient to capture fish eDNA and the approach emphasised the importance of taking multiple water samples of sufficient spatial coverage for detecting species of random or patchy distribution.
Source: Scopus
Laboratory and field validation of a simple method for detecting four species of non-native freshwater fish using eDNA.
Authors: Davison, P.I., Créach, V., Liang, W.-J., Andreou, D., Britton, J.R. and Copp, G.H.
Journal: J Fish Biol
Volume: 89
Issue: 3
Pages: 1782-1793
eISSN: 1095-8649
DOI: 10.1111/jfb.13086
Abstract:This paper presents the first phase in the development and validation of a simple and reliable environmental (e)DNA method using conventional PCR to detect four species of non-native freshwater fish: pumpkinseed Lepomis gibbosus, sunbleak Leucaspius delineatus, fathead minnow Pimephales promelas and topmouth gudgeon Pseudorasbora parva. The efficacy of the approach was demonstrated in indoor tank (44 l) trials in which all four species were detected within 24 h. Validation was through two field trials, in which L. gibbosus was detected 6-12 h after its introduction into outdoor experimental ponds and P. parva was successfully detected in disused fish rearing ponds where the species was known to exist. Thus, the filtration of small (30 ml) volumes of pond water was sufficient to capture fish eDNA and the approach emphasised the importance of taking multiple water samples of sufficient spatial coverage for detecting species of random or patchy distribution.
Source: PubMed
Laboratory and field validation of a simple method for detecting four species of non-native freshwater fish using eDNA
Authors: Davison, P.I., Creach, V., Liang, W.-J., Andreou, D., Britton, J.R. and Copp, G.H.
Journal: JOURNAL OF FISH BIOLOGY
Volume: 89
Issue: 3
Pages: 1782-1793
eISSN: 1095-8649
ISSN: 0022-1112
DOI: 10.1111/jfb.13086
Source: Web of Science (Lite)
Laboratory and field validation of a simple method for detecting four species of non-native freshwater fish using eDNA.
Authors: Davison, P.I., Créach, V., Liang, W.-J., Andreou, D., Britton, J.R. and Copp, G.H.
Journal: Journal of fish biology
Volume: 89
Issue: 3
Pages: 1782-1793
eISSN: 1095-8649
ISSN: 0022-1112
DOI: 10.1111/jfb.13086
Abstract:This paper presents the first phase in the development and validation of a simple and reliable environmental (e)DNA method using conventional PCR to detect four species of non-native freshwater fish: pumpkinseed Lepomis gibbosus, sunbleak Leucaspius delineatus, fathead minnow Pimephales promelas and topmouth gudgeon Pseudorasbora parva. The efficacy of the approach was demonstrated in indoor tank (44 l) trials in which all four species were detected within 24 h. Validation was through two field trials, in which L. gibbosus was detected 6-12 h after its introduction into outdoor experimental ponds and P. parva was successfully detected in disused fish rearing ponds where the species was known to exist. Thus, the filtration of small (30 ml) volumes of pond water was sufficient to capture fish eDNA and the approach emphasised the importance of taking multiple water samples of sufficient spatial coverage for detecting species of random or patchy distribution.
Source: Europe PubMed Central