Preliminary studies on the cryopreservation of red blood cells of rainbow trout (Oncorhynchus mykiss) and European catfish (Silurus glanis L.)

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Authors: Zhang, T., Maisse, G. and Rawson, D.M.

Journal: Cryo-Letters

Volume: 17

Issue: 5

Pages: 303-308

ISSN: 0143-2044

Cryopreservation of red blood cells of rainbow trout (Oncorhynchus mykiss) and European catfish (Silurus glanis L.) was studied using controlled slow cooling. Dimethyl sulfoxide (DMSO) was found to be the least toxic and most effective cryoprotectant when compared with methanol, glycerol and propan-1,2-diol. The best recoveries of intact cells, achieved after cryopreservation under the selected conditions, were 86.3 ± 8.1 and 89.7 ± 7.9 for rainbow trout and catfish respectively. These results open up the possibility of cryopreserved fish red blood cells being used in cytotoxic assays.

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