Production of live larvae following in vitro maturation of zebrafish oocytes
Authors: Silva, L.A., Marques, L.S., Zhang, T., Rodrigues, R.B., Eloy, L.R. and Streit, D.P.
Journal: Theriogenology
Volume: 105
Pages: 115-119
ISSN: 0093-691X
DOI: 10.1016/j.theriogenology.2017.09.021
Abstract:This study aimed to assess the effects of carp pituitary extract (CPE), follicle stimulating hormone (FSH) and luteinizing hormone (LH) on zebrafish oocyte maturation and the ability of these mature oocytes to be fertilized and developed until hatching. Stage III follicles were matured in eight treatments: five concentrations of CPE (16, 32, 48, 64 and 80 μg/mL), one of FSH (0.5 μg/mL), one of LH (0.5 μg/mL), or one combination of FSH (0.5 μg/mL) and LH (0.5 μg/mL). Maturation rates in CPE treatments were 12.8% (16 μg/mL), 24.8% (32 μg/mL), 27.0% (48 μg/mL), 22.7% (64 μg/mL) and 9.6% (80 μg/mL); in FSH was 15.7% (0.5 μg/mL), in LH was 31.8% (0.5 μg/mL) and in FSH (0.5 μg/mL) combined with LH (0.5 μg/mL) it was 50.4%. In vitro fertilization was performed in all treatments; however, only the treatment combining FSH and LH resulted in fertilized oocytes. After maturation using FSH combined with LH, the cleavage rate was 33.3% and hatching rate of live larvae was 20.0%. These results showed that FSH combined with LH was effective in IVM of zebrafish oocyte.
https://eprints.bournemouth.ac.uk/29863/
Source: Scopus
Production of live larvae following in vitro maturation of zebrafish oocytes.
Authors: Silva, L.A., Marques, L.S., Zhang, T., Rodrigues, R.B., Eloy, L.R. and Streit, D.P.
Journal: Theriogenology
Volume: 105
Pages: 115-119
eISSN: 1879-3231
DOI: 10.1016/j.theriogenology.2017.09.021
Abstract:This study aimed to assess the effects of carp pituitary extract (CPE), follicle stimulating hormone (FSH) and luteinizing hormone (LH) on zebrafish oocyte maturation and the ability of these mature oocytes to be fertilized and developed until hatching. Stage III follicles were matured in eight treatments: five concentrations of CPE (16, 32, 48, 64 and 80 μg/mL), one of FSH (0.5 μg/mL), one of LH (0.5 μg/mL), or one combination of FSH (0.5 μg/mL) and LH (0.5 μg/mL). Maturation rates in CPE treatments were 12.8% (16 μg/mL), 24.8% (32 μg/mL), 27.0% (48 μg/mL), 22.7% (64 μg/mL) and 9.6% (80 μg/mL); in FSH was 15.7% (0.5 μg/mL), in LH was 31.8% (0.5 μg/mL) and in FSH (0.5 μg/mL) combined with LH (0.5 μg/mL) it was 50.4%. In vitro fertilization was performed in all treatments; however, only the treatment combining FSH and LH resulted in fertilized oocytes. After maturation using FSH combined with LH, the cleavage rate was 33.3% and hatching rate of live larvae was 20.0%. These results showed that FSH combined with LH was effective in IVM of zebrafish oocyte.
https://eprints.bournemouth.ac.uk/29863/
Source: PubMed
Production of live larvae following in vitro maturation of zebrafish oocytes.
Authors: Silva, L.A., Marques, L.S., Zhang, T., Rodrigues, R.B., Eloy, L.R. and Streit, D.P.
Journal: Theriogenology
Volume: 105
Pages: 115-119
eISSN: 1879-3231
ISSN: 0093-691X
DOI: 10.1016/j.theriogenology.2017.09.021
Abstract:This study aimed to assess the effects of carp pituitary extract (CPE), follicle stimulating hormone (FSH) and luteinizing hormone (LH) on zebrafish oocyte maturation and the ability of these mature oocytes to be fertilized and developed until hatching. Stage III follicles were matured in eight treatments: five concentrations of CPE (16, 32, 48, 64 and 80 μg/mL), one of FSH (0.5 μg/mL), one of LH (0.5 μg/mL), or one combination of FSH (0.5 μg/mL) and LH (0.5 μg/mL). Maturation rates in CPE treatments were 12.8% (16 μg/mL), 24.8% (32 μg/mL), 27.0% (48 μg/mL), 22.7% (64 μg/mL) and 9.6% (80 μg/mL); in FSH was 15.7% (0.5 μg/mL), in LH was 31.8% (0.5 μg/mL) and in FSH (0.5 μg/mL) combined with LH (0.5 μg/mL) it was 50.4%. In vitro fertilization was performed in all treatments; however, only the treatment combining FSH and LH resulted in fertilized oocytes. After maturation using FSH combined with LH, the cleavage rate was 33.3% and hatching rate of live larvae was 20.0%. These results showed that FSH combined with LH was effective in IVM of zebrafish oocyte.
https://eprints.bournemouth.ac.uk/29863/
Source: Europe PubMed Central
Production of live larvae following in vitro maturation of zebrafish oocytes.
Authors: Silva, L.A., Marques, L.S., Zhang, T., Rodrigues, R.B., Eloy, L.R. and Streit Jr., D.P.
Journal: Theriogenology
Volume: 105
Pages: 115-119
ISSN: 0093-691X
Abstract:This study aimed to assess the effects of carp pituitary extract (CPE), follicle stimulating hormone (FSH) and luteinizing hormone (LH) on zebrafish oocyte maturation and the ability of these mature oocytes to be fertilized and developed until hatching. Stage III follicles were matured in eight treatments: five concentrations of CPE (16, 32, 48, 64 and 80 μg/mL), one of FSH (0.5 μg/mL), one of LH (0.5 μg/mL), or one combination of FSH (0.5 μg/mL) and LH (0.5 μg/mL). Maturation rates in CPE treatments were 12.8% (16 μg/mL), 24.8% (32 μg/mL), 27.0% (48 μg/mL), 22.7% (64 μg/mL) and 9.6% (80 μg/mL); in FSH was 15.7% (0.5 μg/mL), in LH was 31.8% (0.5 μg/mL) and in FSH (0.5 μg/mL) combined with LH (0.5 μg/mL) it was 50.4%. In vitro fertilization was performed in all treatments; however, only the treatment combining FSH and LH resulted in fertilized oocytes. After maturation using FSH combined with LH, the cleavage rate was 33.3% and hatching rate of live larvae was 20.0%. These results showed that FSH combined with LH was effective in IVM of zebrafish oocyte.
https://eprints.bournemouth.ac.uk/29863/
Source: BURO EPrints