Waterborne zinc bioaccumulation influences glucose metabolism in orange-spotted grouper embryos
Authors: Zeng, H., Zhang, P., Ye, H., Ji, Y., Hogstrand, C., Green, I., Xiao, J., Fu, Q. and Guo, Z.
Journal: Environmental Pollution
Volume: 283
eISSN: 1873-6424
ISSN: 0269-7491
DOI: 10.1016/j.envpol.2021.117325
Abstract:Fish embryos, as an endogenous system, strictly regulate an energy metabolism that is particularly sensitive to environmental pressure. This study used orange-spotted grouper embryos and stable isotope 67Zn to test the hypothesis that waterborne Zn exposure had a significant effect on energy metabolism in embryos. The fish embryos were exposed to a gradient level of waterborne 67Zn, and then sampled to quantify 67Zn bioaccumulation and mRNA expressions of key genes involved glucose metabolism. The results indicated that the bioaccumulated 67Zn generally increased with increasing waterborne 67Zn concentrations, while it tended to be saturated at waterborne 67Zn > 0.7 mg L−1. As we hypothesized, the expression of PK and PFK gene involved glycolysis pathway was significantly up-regulated under waterborne 67Zn exposure >4 mg L−1. Waterborne 67Zn exposure >2 mg L−1 significantly suppressed PCK and G6PC gene expression involved gluconeogenesis pathway, and also inhibited the AKT2, GSK-3beta and GLUT4 genes involved Akt signaling pathway. Our findings first characterized developmental stage-dependent Zn uptake and genotoxicity in fish embryos. We suggest fish embryos, as a small-scale modeling biosystem, have a large potential and wide applicability for determining cytotoxicity/genotoxicity of waterborne metal in aquatic ecosystem.
Source: Scopus
Waterborne zinc bioaccumulation influences glucose metabolism in orange-spotted grouper embryos.
Authors: Zeng, H., Zhang, P., Ye, H., Ji, Y., Hogstrand, C., Green, I., Xiao, J., Fu, Q. and Guo, Z.
Journal: Environ Pollut
Volume: 283
Pages: 117325
eISSN: 1873-6424
DOI: 10.1016/j.envpol.2021.117325
Abstract:Fish embryos, as an endogenous system, strictly regulate an energy metabolism that is particularly sensitive to environmental pressure. This study used orange-spotted grouper embryos and stable isotope 67Zn to test the hypothesis that waterborne Zn exposure had a significant effect on energy metabolism in embryos. The fish embryos were exposed to a gradient level of waterborne 67Zn, and then sampled to quantify 67Zn bioaccumulation and mRNA expressions of key genes involved glucose metabolism. The results indicated that the bioaccumulated 67Zn generally increased with increasing waterborne 67Zn concentrations, while it tended to be saturated at waterborne 67Zn > 0.7 mg L-1. As we hypothesized, the expression of PK and PFK gene involved glycolysis pathway was significantly up-regulated under waterborne 67Zn exposure >4 mg L-1. Waterborne 67Zn exposure >2 mg L-1 significantly suppressed PCK and G6PC gene expression involved gluconeogenesis pathway, and also inhibited the AKT2, GSK-3beta and GLUT4 genes involved Akt signaling pathway. Our findings first characterized developmental stage-dependent Zn uptake and genotoxicity in fish embryos. We suggest fish embryos, as a small-scale modeling biosystem, have a large potential and wide applicability for determining cytotoxicity/genotoxicity of waterborne metal in aquatic ecosystem.
Source: PubMed
Waterborne zinc bioaccumulation influences glucose metabolism in orange-spotted grouper embryos
Authors: Zeng, H., Zhang, P., Ye, H., Ji, Y., Hogstrand, C., Green, I., Xiao, J., Fu, Q. and Guo, Z.
Journal: ENVIRONMENTAL POLLUTION
Volume: 283
eISSN: 1873-6424
ISSN: 0269-7491
DOI: 10.1016/j.envpol.2021.117325
Source: Web of Science (Lite)
Waterborne zinc bioaccumulation influences glucose metabolism in orange-spotted grouper embryos.
Authors: Zeng, H., Zhang, P., Ye, H., Ji, Y., Hogstrand, C., Green, I., Xiao, J., Fu, Q. and Guo, Z.
Journal: Environmental pollution (Barking, Essex : 1987)
Volume: 283
Pages: 117325
eISSN: 1873-6424
ISSN: 0269-7491
DOI: 10.1016/j.envpol.2021.117325
Abstract:Fish embryos, as an endogenous system, strictly regulate an energy metabolism that is particularly sensitive to environmental pressure. This study used orange-spotted grouper embryos and stable isotope 67Zn to test the hypothesis that waterborne Zn exposure had a significant effect on energy metabolism in embryos. The fish embryos were exposed to a gradient level of waterborne 67Zn, and then sampled to quantify 67Zn bioaccumulation and mRNA expressions of key genes involved glucose metabolism. The results indicated that the bioaccumulated 67Zn generally increased with increasing waterborne 67Zn concentrations, while it tended to be saturated at waterborne 67Zn > 0.7 mg L-1. As we hypothesized, the expression of PK and PFK gene involved glycolysis pathway was significantly up-regulated under waterborne 67Zn exposure >4 mg L-1. Waterborne 67Zn exposure >2 mg L-1 significantly suppressed PCK and G6PC gene expression involved gluconeogenesis pathway, and also inhibited the AKT2, GSK-3beta and GLUT4 genes involved Akt signaling pathway. Our findings first characterized developmental stage-dependent Zn uptake and genotoxicity in fish embryos. We suggest fish embryos, as a small-scale modeling biosystem, have a large potential and wide applicability for determining cytotoxicity/genotoxicity of waterborne metal in aquatic ecosystem.
Source: Europe PubMed Central