A Moving Source of Matrix Components Is Essential for De Novo Basement Membrane Formation
Authors: Matsubayashi, Y. et al.
Journal: Current Biology
Volume: 27
Issue: 22
Pages: 3526-3534.e4
ISSN: 0960-9822
DOI: 10.1016/j.cub.2017.10.001
Abstract:The basement membrane (BM) is a thin layer of extracellular matrix (ECM) beneath nearly all epithelial cell types that is critical for cellular and tissue function. It is composed of numerous components conserved among all bilaterians [1]; however, it is unknown how all of these components are generated and subsequently constructed to form a fully mature BM in the living animal. Although BM formation is thought to simply involve a process of self-assembly [2], this concept suffers from a number of logistical issues when considering its construction in vivo. First, incorporation of BM components appears to be hierarchical [3–5], yet it is unclear whether their production during embryogenesis must also be regulated in a temporal fashion. Second, many BM proteins are produced not only by the cells residing on the BM but also by surrounding cell types [6–9], and it is unclear how large, possibly insoluble protein complexes [10] are delivered into the matrix. Here we exploit our ability to live image and genetically dissect de novo BM formation during Drosophila development. This reveals that there is a temporal hierarchy of BM protein production that is essential for proper component incorporation. Furthermore, we show that BM components require secretion by migrating macrophages (hemocytes) during their developmental dispersal, which is critical for embryogenesis. Indeed, hemocyte migration is essential to deliver a subset of ECM components evenly throughout the embryo. This reveals that de novo BM construction requires a combination of both production and distribution logistics allowing for the timely delivery of core components. Drosophila macrophages undergo stereotyped developmental migrations to evenly populate the embryo. Matsubayashi et al. reveal that macrophages are the biggest producers of extracellular matrix in the embryo and that their dispersal is essential to evenly distribute a subset of matrix components during development.
Source: Scopus
A Moving Source of Matrix Components Is Essential for De Novo Basement Membrane Formation.
Authors: Matsubayashi, Y. et al.
Journal: Curr Biol
Volume: 27
Issue: 22
Pages: 3526-3534.e4
eISSN: 1879-0445
DOI: 10.1016/j.cub.2017.10.001
Abstract:The basement membrane (BM) is a thin layer of extracellular matrix (ECM) beneath nearly all epithelial cell types that is critical for cellular and tissue function. It is composed of numerous components conserved among all bilaterians [1]; however, it is unknown how all of these components are generated and subsequently constructed to form a fully mature BM in the living animal. Although BM formation is thought to simply involve a process of self-assembly [2], this concept suffers from a number of logistical issues when considering its construction in vivo. First, incorporation of BM components appears to be hierarchical [3-5], yet it is unclear whether their production during embryogenesis must also be regulated in a temporal fashion. Second, many BM proteins are produced not only by the cells residing on the BM but also by surrounding cell types [6-9], and it is unclear how large, possibly insoluble protein complexes [10] are delivered into the matrix. Here we exploit our ability to live image and genetically dissect de novo BM formation during Drosophila development. This reveals that there is a temporal hierarchy of BM protein production that is essential for proper component incorporation. Furthermore, we show that BM components require secretion by migrating macrophages (hemocytes) during their developmental dispersal, which is critical for embryogenesis. Indeed, hemocyte migration is essential to deliver a subset of ECM components evenly throughout the embryo. This reveals that de novo BM construction requires a combination of both production and distribution logistics allowing for the timely delivery of core components.
Source: PubMed
A Moving Source of Matrix Components Is Essential for <i>De Novo</i> Basement Membrane Formation
Authors: Matsubayashi, Y. et al.
Journal: CURRENT BIOLOGY
Volume: 27
Issue: 22
Pages: 3526-+
eISSN: 1879-0445
ISSN: 0960-9822
DOI: 10.1016/j.cub.2017.10.001
Source: Web of Science (Lite)
A Moving Source of Matrix Components Is Essential for De Novo Basement Membrane Formation.
Authors: Matsubayashi, Y. et al.
Journal: Current biology : CB
Volume: 27
Issue: 22
Pages: 3526-3534.e4
eISSN: 1879-0445
ISSN: 0960-9822
DOI: 10.1016/j.cub.2017.10.001
Abstract:The basement membrane (BM) is a thin layer of extracellular matrix (ECM) beneath nearly all epithelial cell types that is critical for cellular and tissue function. It is composed of numerous components conserved among all bilaterians [1]; however, it is unknown how all of these components are generated and subsequently constructed to form a fully mature BM in the living animal. Although BM formation is thought to simply involve a process of self-assembly [2], this concept suffers from a number of logistical issues when considering its construction in vivo. First, incorporation of BM components appears to be hierarchical [3-5], yet it is unclear whether their production during embryogenesis must also be regulated in a temporal fashion. Second, many BM proteins are produced not only by the cells residing on the BM but also by surrounding cell types [6-9], and it is unclear how large, possibly insoluble protein complexes [10] are delivered into the matrix. Here we exploit our ability to live image and genetically dissect de novo BM formation during Drosophila development. This reveals that there is a temporal hierarchy of BM protein production that is essential for proper component incorporation. Furthermore, we show that BM components require secretion by migrating macrophages (hemocytes) during their developmental dispersal, which is critical for embryogenesis. Indeed, hemocyte migration is essential to deliver a subset of ECM components evenly throughout the embryo. This reveals that de novo BM construction requires a combination of both production and distribution logistics allowing for the timely delivery of core components.
Source: Europe PubMed Central