Endocytosis-dependent coordination of multiple actin regulators is required for wound healing.
Authors: Matsubayashi, Y., Coulson-Gilmer, C. and Millard, T.H.
Journal: J Cell Biol
Volume: 210
Issue: 3
Pages: 419-433
eISSN: 1540-8140
DOI: 10.1083/jcb.201411037
Abstract:The ability to heal wounds efficiently is essential for life. After wounding of an epithelium, the cells bordering the wound form dynamic actin protrusions and/or a contractile actomyosin cable, and these actin structures drive wound closure. Despite their importance in wound healing, the molecular mechanisms that regulate the assembly of these actin structures at wound edges are not well understood. In this paper, using Drosophila melanogaster embryos, we demonstrate that Diaphanous, SCAR, and WASp play distinct but overlapping roles in regulating actin assembly during wound healing. Moreover, we show that endocytosis is essential for wound edge actin assembly and wound closure. We identify adherens junctions (AJs) as a key target of endocytosis during wound healing and propose that endocytic remodeling of AJs is required to form "signaling centers" along the wound edge that control actin assembly. We conclude that coordination of actin assembly, AJ remodeling, and membrane traffic is required for the construction of a motile leading edge during wound healing.
Source: PubMed
Endocytosis-dependent coordination of multiple actin regulators is required for wound healing
Authors: Matsubayashi, Y., Coulson-Gilmer, C. and Millard, T.H.
Journal: JOURNAL OF CELL BIOLOGY
Volume: 210
Issue: 3
Pages: 419-433
eISSN: 1540-8140
ISSN: 0021-9525
DOI: 10.1083/jcb.201411037
Source: Web of Science (Lite)
Endocytosis-dependent coordination of multiple actin regulators is required for wound healing.
Authors: Matsubayashi, Y., Coulson-Gilmer, C. and Millard, T.H.
Journal: The Journal of cell biology
Volume: 210
Issue: 3
Pages: 419-433
eISSN: 1540-8140
ISSN: 0021-9525
DOI: 10.1083/jcb.201411037
Abstract:The ability to heal wounds efficiently is essential for life. After wounding of an epithelium, the cells bordering the wound form dynamic actin protrusions and/or a contractile actomyosin cable, and these actin structures drive wound closure. Despite their importance in wound healing, the molecular mechanisms that regulate the assembly of these actin structures at wound edges are not well understood. In this paper, using Drosophila melanogaster embryos, we demonstrate that Diaphanous, SCAR, and WASp play distinct but overlapping roles in regulating actin assembly during wound healing. Moreover, we show that endocytosis is essential for wound edge actin assembly and wound closure. We identify adherens junctions (AJs) as a key target of endocytosis during wound healing and propose that endocytic remodeling of AJs is required to form "signaling centers" along the wound edge that control actin assembly. We conclude that coordination of actin assembly, AJ remodeling, and membrane traffic is required for the construction of a motile leading edge during wound healing.
Source: Europe PubMed Central