Tiantian Zhang

Professor Tiantian Zhang

  • 01202 965721
  • tzhang at bournemouth dot ac dot uk
  • Deputy Dean Research & Professional Practice
  • Poole House P307, Talbot Campus, Fern Barrow, Poole, BH12 5BB
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Biography

Professor Tiantian Zhang obtained her Environmental Biology degree from Liaoning University (P.R. China) in 1982 and worked as a research scientist in the area of environmental biology before obtaining an MPhil degree in Environmental Biology at Middlesex University (UK) in 1990 and a PhD degree in Cryobiology at University of Bedfordshire in 1994 (UK). She worked as a post-doctoral research fellow and a senior research fellow at University of Bedfordshire before she was made Reader in 2003 and Professor in 2005. She was appointed Director of Institute of Research in the Applied Natural Sciences at University of Bedfordshire in 2008 before joining Bournemouth University in 2012 as the Head of the Graduate School. She is also a member of the Executive Committee of the UK Council for Graduate Education.

Professor Tiantian Zhang is a leading figure internationally in research on cryopreservation of gametes and embryos of fish species and her research interests have been in the areas of cryopreservation of reproductive cells and the effect of cryopreservation on genome integrity and cellular metabolism...

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Research

My current research interests and activities are in the areas of the cryopreservation of reproductive cells, tissues and embryos of aquatic species and studies on the effect of cryopreservation on genome and cellular metabolism of reproductive cells including stem cells. Although successful cryopreservation of sperm has been achieved with many mammalian species including humans, the successful cryopreservation of fish oocytes and embryos remained elusive. Successful cryopreservation of fish oocytes and embryos will have important applications in conservation of biodiversity, aquaculture and genomic studies, and research in this area remains an intellectual challenge. My research has contributed significantly to an understanding of the problems associated with fish oocyte and embryo cryopreservation, and will undoubtedly assist successful protocol design in the future. My earlier research also included studies on the effect of temperature on cytotoxicity in prokaryotic cells and ecotoxicological studies using fish cell lines and other biological materials.

Journal Articles

  • Silva, L.A., Marques, L.S., Zhang, T., Rodrigues, R.B., Eloy, L.R. and Streit, D.P., 2018. Production of live larvae following in vitro maturation of zebrafish oocytes. Theriogenology, 105, 115-119.
  • Zhang, T., 2017. Importance of cryobanking in aquatic species conservation and aquaculture. Cryobiology.
  • Sana, S., Hardouin, E.A., Gozlan, R.E., Ercan, D., Tarkan, A.S., Zhang, T. and Andreou, D., 2017. Origin and invasion of the emerging infectious pathogen Sphaerothecum destruens. EMERGING MICROBES & INFECTIONS, 6.
  • Martínez-Páramo, S., Zhang, T. et al., 2017. Cryobanking of aquatic species. Aquaculture, 472, 156-177.
  • Anil, S., Rawson, D.M. and Zhang, T., 2016. In-vitro culture protocol development for early stage zebrafish ovarian follicles. Cryobiology, 73 (3), 433.
  • Marques, L.S., Bos-Mikich, A., Godoy, L.C., Silva, L.A., Maschio, D., Zhang, T. and Streit, D.P., 2015. Viability of zebrafish (Danio rerio) ovarian follicles after vitrification in a metal container. Cryobiology, 71 (3), 367-373.
  • Desai, K., Spikings, E. and Zhang, T., 2015. Use of methanol as cryoprotectant and its effect on sox genes and proteins in chilled zebrafish embryos. Cryobiology, 71 (1), 1-11.
  • Chantzaropoulos, A., Nathanailides, C., Kokokiris, L., Barbouti, A. and Zhang, T., 2015. A brief exposure to low pH prior to refrigerated storage reduces the motility and viability of goldfish sperm (Carassius auratus, Linnaeus, 1758). JOURNAL OF APPLIED ICHTHYOLOGY, 31, 89-93.
  • Desai, K., Spikings, E. and Zhang, T., 2015. Short-term chilled storage of zebrafish (Danio rerio) embryos in cryoprotectant as an alternative to cryopreservation. Zebrafish, 12 (1), 111-120.
  • Ahmed, R., Spikings, E., Zhou, S., Thompsett, A. and Zhang, T., 2014. Pre-hybridisation: An efficient way of suppressing endogenous biotin-binding activity inherent to biotin-streptavidin detection system. Journal of Immunological Methods, 406, 143-147.
  • Godoy, L.C., Streit, D.P., Zampolla, T., Bos-Mikich, A. and Zhang, T., 2013. A study on the vitrification of stage III zebrafish (Danio rerio) ovarian follicles. Cryobiology, 67 (3), 347-354.
  • Ahmed, R., Spikings, E., Thompsett, A., Zhou, S., Zhang, T. and Zhang, T., 2013. Investigation of the effects of different cryopreservation parameters on the genome of zebrafish (Danio rerio) embryos. Cryobiology. Cryobiology, 67 (3), 433-434.
  • Anil, S., Rawson, D.M., Zhang, T. and Zhang, T., 2013. Development of cryopreservation protocol for zebrafish ovarian fragments using controlled slow cooling. Cryobiology, 67 (3), 433.
  • Salavati, M., Ghafari, F., Zhang, T. and Fouladi-Nashta, A.A., 2013. Influence of caffeine pretreatment on biphasic invitro maturation of dog oocytes. Theriogenology, 80 (7), 784-792.
  • Anil, S., Zampolla, T. and Zhang, T., 2012. Development of molecular markers for stage I and stage II zebrafish ovarian follicles after in vitro culture. Cryobiology, 65 (3), 360.
  • Godoy, L., Zampolla, T., Streit Jr, D., Bos-Mikich, A. and Zhang, T., 2012. Vitrification of zebrafish (Danio rerio) ovarian follicles. Cryobiology, 65 (3), 344.
  • Salavati, M., Ghafari, F., Zhang, T. and Fouladi-Nashta, A.A., 2012. Effects of oxygen concentration on in vitro maturation of canine oocytes in a chemically defined serum-free medium. Reproduction, 144 (5), 547-556.
  • Zampolla, T., Rawson, D.M. and Zhang, T., 2012. The use of fetal bovine serum for cryopreservation of stage III zebrafish (Danio rerio) ovarian follicles. Cryo Letters, 33 (6), 435-442.
  • Wang, L., Zhang, T.T. et al., 2012. First live offspring born in superovulated sika deer (Cervus nippon) after embryo vitrification. Theriogenology, 78 (7), 1627-1632.
  • Spikings, E., Zampolla, T., Rawson, D., Wang, Y. and Zhang, T., 2012. Effect of methanol on mitochondrial organization in zebrafish (Danio rerio) ovarian follicles. Theriogenology, 77 (1), 28-38.
  • Nathanailides, C., Chanzaropoulos, T., Barbouti, A., Perdikaris, C. and Zhang, T., 2011. DNA fragmentation, linear velocity and fertilising ability of reactivated cry op reserved goldfish sperm using different cryoprotectants. Biotechnology, 10 (6), 514-520.
  • Desai, K., Spikings, E. and Zhang, T., 2011. Studies on the role of hsp47 and hsp90 in zebrafish (Danio rerio) embryos under chilled conditions. Cryobiology, 63 (3), 334.
  • Anil, T., Zampolla, T. and Zhang, T., 2011. Development of in vitro culture method for zebrafish ovarian tissute fragments. Cryobiology, 63 (3), 311.
  • Zhang, T., 2011. Cryopreservation of fish gametes and its application in aquaculture and conservation. Cryobiology, 63 (3), 314.
  • Zampolla, T., Spikings, E., Srirangarajah, S., Rawson, D.M. and Zhang, T., 2011. Impact of cryoprotectants and cryopreservation on metabolic activity and cytoskeleton proteins of zebrafish (Danio rerio) ovarian fragments. Cryo Letters, 32 (6), 525-536.
  • Desai, K., Spikings, E. and Zhang, T., 2011. Effect of chilling on sox2, sox3 and sox19a gene expression in zebrafish (Danio rerio) embryos. Cryobiology, 63 (2), 96-103.
  • Zampolla, T., Spikings, E., Rawson, D. and Zhang, T., 2011. Cytoskeleton proteins F-actin and tubulin distribution and interaction with mitochondria in the granulosa cells surrounding stage III zebrafish (Danio rerio) oocytes. Theriogenology, 76 (6), 1110-1119.
  • Lin, C., Zhang, T., Kuo, F.W. and Tsai, S., 2011. Gorgonian coral (Junceella juncea and Junceella fragilis) oocyte chilling sensitivity in the context of adenosine triphosphate response (ATP). Cryo-Letters, 32 (2), 141-147.
  • Zhang, T., 2011. Cryopreservation of fish gamates and it’s applications in aquaculture and conservation. Toxicology and Environmental Health Sciences, 3, S30.
  • Anil, S., Ghafari, F., Zampolla, T., Rawson, D.M. and Zhang, T., 2011. Studies on cryoprotectant toxicity to zebrafish (Danio rerio) ovarian tissue fragments. Cryo Letters, 32 (1), 40-50.
  • Spikings, E., Vishnolia, K., Rawson, D.M. and Zhang, T., 2010. Effect of methanol and chilling on expression of mitochondrial DNA replication factors and mitochondrial DNA replication in zebrafish embryos. Cryobiology, 61 (3), 391.
  • Anil, S., Ghafari, F., Zampolla, T., Rawson, D.M. and Zhang, T., 2010. Studies on cryoprotectant toxicity to zebrafish (Danio rerio) ovarian tissue fragments. Cryobiology, 61 (3), 384.
  • Desai, K., Spikings, E., Rawson, D.M. and Zhang, T., 2010. Studies on impact of chilling on sox gene expression in zebrafish (Danio rerio) embryos. Cryobiology, 61 (3), 389.
  • Zampolla, T., Spikings, E., Zhang, T. and Rawson, D., 2010. Investigation of methanol impact on mitochondrial transport and cytoskeleton proteins in stage III zebrafish (Danio rerio) ovarian follicles. Cryobiology, 61 (3), 385.
  • Tsai, S., Rawson, D.M. and Zhang, T., 2010. Development of in vitro culture method for early stage zebrafish (Danio rerio) ovarian follicles for use in cryopreservation studies. Theriogenology, 74 (2), 290-303.
  • Guan, M., Rawson, D.M. and Zhang, T., 2010. Cryopreservation of zebrafish (Danio rerio) oocytes by vitrification. Cryo Letters, 31 (3), 230-238.
  • Rawson, D., Zhang, T. and Zampolla, T., 2010. Cryo-banking of cells and tissues from freshwater and marine fish in the UK waters. Biopreservation and Biobanking, 7 (4), 185.
  • Zampolla, T., Spikings, E., Zhang, T. and Rawson, D.M., 2009. Effect of methanol and DMSO exposure on mitochondrial activity and distribution in stage III ovarian follicles of zebrafish (Danio rerio). Cryobiology, 59 (3), 413.
  • Ghafari, F., Spikings, E., Rawson, D.M. and Zhang, T., 2009. Effect of cryoprotectant treatment and chilling on oxidative stress in zebrafish (Danio rerio) early ovarian follicles. Cryobiology, 59 (3), 415.
  • Zampolla, T., Spikings, E., Zhang, T. and Rawson, D.M., 2009. Effect of methanol and Me2SO exposure on mitochondrial activity and distribution in stage III ovarian follicles of zebrafish (Danio rerio). Cryobiology, 59 (2), 188-194.
  • Lin, C., Spikings, E., Zhang, T. and Rawson, D.M., 2009. Effect of chilling and cryopreservation on expression of Pax genes in zebrafish (Danio rerio) embryos and blastomeres. Cryobiology, 59 (1), 42-47.
  • Zampolla, T., Zhang, T., Holt, W.V. and Rawson, D.M., 2009. Distributional arrangement of mitochondria in the granulosa cells surrounding stage III zebrafish (Danio rerio) oocytes. Theriogenology, 72 (1), 111-119.
  • Tsai, S., Rawson, D.M. and Zhang, T., 2009. Studies on chilling sensitivity of early stage zebrafish (Danio rerio) ovarian follicles. Cryobiology, 58 (3), 279-286.
  • Tsai, S., Rawson, D.M. and Zhang, T., 2009. Development of cryopreservation protocols for early stage zebrafish (Danio rerio) ovarian follicles using controlled slow cooling. Theriogenology, 71 (8), 1226-1233.
  • Lin, C., Spikings, E., Zhang, T. and Rawson, D., 2009. Housekeeping genes for cryopreservation studies on zebrafish embryos and blastomeres. Theriogenology, 71 (7), 1147-1155.
  • Lin, C., Zhang, T. and Rawson, D.M., 2009. Cryopreservation of zebrafish (Danio rerio) blastomeres by controlled slow cooling. Cryo Letters, 30 (2), 132-141.
  • Spikings, E., Zhang, T. and Rawson, D., 2009. Effect of methanol on expression of mitochondrial DNA replication factors and mitochondrial DNA replication in zebrafish embryos. Cryoletters, 31 (2), 173-174.
  • Lin, C., Spikings, E., Zhang, T. and Rawson, D., 2009. Evaluation of potential housekeeping genes for use in expression profiling the effects of chilling and freezing in zebrafish embryos and blastomeres. Cryoletters, 30 (2), 157.
  • Spikings, E., Zhang, T. and Rawson, D., 2009. The effect of chilling on replication of mitochondrial DNA in zebrafish embryos. Cryoletters, 30 (2), 159.
  • Zampolla, T., Spikings, E., Zhang, T. and Rawson, D., 2009. Effect of cryoprotectants exposure on mitochondrial activity and mtDNA copy number. Cryoletters, 30 (2), 160.
  • Tsai, S., Rawson, D. and Zhang, T., 2009. Development of cryopreservation protocols for early stage zebrafish (Danio rerio) ovarian follicles using controlled slow cooling. Cryoletters, 30 (2), 162-163.
  • Rawson, D.M. and Zhang, T., 2009. Cryopreservation progress, challenges and applications in aquatic species. Cryoletters, 30 (1), 78.
  • Davies, J., Zhang, T., Fuller, B. and Pillai, R., 2009. TO PERFORM A PILOT STUDY TO DEVELOP A QUALITY ASSESSMENT PROTOCOL FOR OPTIMISATION STUDIES AND FOR ROUTINE POST CRYOPRESERVATION USE IN CARDIOVASCULAR TISSUE BANKING. CRYOLETTERS, 30 (2), 153.
  • Chanzaropoulos, T., Nathanailides, C. and Zhang, T., 2008. Computer assisted speam motility analysis (CASA) of goldfish after cryopreservation. Proceedings of 4th International Congress on Aquaculture, Fisheries Technology and Environmental Management.
  • Ding, Y., Zhang, T. and Rawson, D.M., 2008. Mediated amperometric whole-cell biosensors for rapid in situ screening of heavy metal contaminated soil. Proceedings of 3rd International Conference on Soil Pollution and Remediation, 209-215.
  • Tsai, S., Rawson, D.M. and Zhang, T., 2008. Studies on cryoprotectant toxicity to early stage zebrafish (Danio rerio) ovarian follicles. Cryo Letters, 29 (6), 477-483.
  • Zampolla, T., Zhang, T. and Rawson, D.M., 2008. Evaluation of zebrafish (Danio rerio) ovarian follicle viability by simultaneous staining with fluorescein diacetate and propidium iodide. Cryo Letters, 29 (6), 463-475.
  • Babin, P.J., André, M., Forgue, J., Knoll-Gellida, A., Rawson, D.M. and Zhang, T., 2008. Development of oocyte viability molecular signature (OVMS) assays for zebrafish (Danio rerio) oocyte cryopreservation studies. Cybium, 32 (2 SUPPL.), 217.
  • Wang, R.Y., Guan, M., Rawson, D.M. and Zhang, T., 2008. Ultrasound enhanced methanol penetration of zebrafish (Danio rerio) embryos measured by permittivity changes using impedance spectroscopy. Eur Biophys J, 37 (6), 1039-1044.
  • Guan, M., Rawson, D.M. and Zhang, T., 2008. Cryopreservation of zebrafish (Danio rerio) oocytes using improved controlled slow cooling protocols. Cryobiology, 56 (3), 204-208.
  • Zhang, T., Rawson, D.M., Tosti, L. and Carnevali, O., 2008. Cathepsin activities and membrane integrity of zebrafish (Danio rerio) oocytes after freezing to -196 degrees C using controlled slow cooling. Cryobiology, 56 (2), 138-143.
  • Guan, M., Rawson, D.M. and Zhang, T., 2008. Development of a new method for isolating zebrafish oocytes (Danio rerio) from ovary tissue masses. Theriogenology, 69 (3), 269-275.
  • Tsai, S., Rawson, D.M. and Zhang, T., 2008. Studies on cryoprotectant toxicity of early stage zebrafish (Danio rerio) oocytes. CRYOLETTERS, 29 (1), 84-85.
  • Rawson, D.M. and Zhang, T., 2008. The Frozen Ark project. CRYOLETTERS, 29 (1), 78.
  • Babin, P.J., Andre, M., Forgue, J., Knoll-Gellida, A., Rawson, D.M. and Zhang, T., 2008. Development of oocyte viability molecular signature (OVMS) assays for zebrafish (Danio rerio) oocyte cryopreservation studies. Cybium, 32 (2), 217.
  • Lin, C.S., Zhang, T. and Rawson, D.M., 2008. Cryopreservation and gene expression in zebrafish blastomeres. CryoLetters, 29 (1), 85.
  • Zhang, T. and Rawson, D.M., 2007. The Frozen Ark: Cryo-banking of genetic and cellular material of endangered species. Cryobiology, 55, 355.
  • Zhang, T. and Rawson, D.M., 2007. Cryo-conservation in fish: the potentials and challenges. Cryobiology, 55, 354.
  • Lin, C.S., Zhang, T., Elgar, G. and Rawson, D.M., 2007. Initial studies on cryopreservation and gene expression in isolated blastomeres of zebrafish (Danio rerio). Cryobiology, 55, 365.
  • Tsai, S., Rawson, D.M. and Zhang, T., 2007. Studies on chilling sensitivity of early stage zebrafish (Danion rerio) oocytes. Cryobiology, 55, 364-365.
  • Guan, T., Zhang, T. and Rawson, D.M., 2006. Effects of improved controlled slow cooling protocols on the survival of zebrafish (Danio rerio) oocytes. Cryobiology, 53, 377-378.
  • Guan, M., Zhang, T. and Rawson, D.M., 2006. Successful enzymatic separation of zebrafish (Danio rerio) oocytes using hyaluronidase. Cryobiology, 53, 418.
  • Kopeika, J., Zhang, T. and Rawson, D., 2006. Zebrafish embryos (Danio rerio) using microinjection. Cryo Letters, 27 (5), 319-328.
  • Zhang, T., Wang, R.Y., Bao, Q.-Y. and Rawson, D.M., 2006. Development of a new rapid measurement technique for fish embryo membrane permeability studies using impedance spectroscopy. Theriogenology, 66 (4), 982-988.
  • Wex, H., Rawson, D.M. and Zhang, T., 2006. Use of biosensor and impedance spectroscopy assays to investigate the influence of temperature on E. coli sensitivity to 3,5-dichlorophenol. Electrochimica Acta, 51 (24), 5157-5162.
  • Wang, R.Y., Zhang, T., Bao, Q. and Rawson, D.M., 2006. Study on fish embryo responses to the treatment of cryoprotective chemicals using impedance spectroscopy. Eur Biophys J, 35 (3), 224-230.
  • Plachinta, M., Zhang, T. and Rawson, D.M., 2005. Studies on the effect of certain supplements in cryoprotective medium on zebrafish (Danio rerio) oocytes quality after controlled slow cooling. Cryobiology, 51, 405.
  • Kopeika, J., Zhang, T. and Rawson, D.M., 2005. Preliminary study on modification of yolk sec of zebrafish embryos (Danio rerio) using micro-injection. Cryobiology, 51, 405-406.
  • Rawson, D.M., Zhang, T., Wex, H. and Bhatia, R., 2005. Cell based biosensors for environmental monitoring: challenges in exploiting cell bioassays in disposable sensor configurations. Cryobiology, 51, 384-385.
  • Zhang, T., Plachinta, M., Kopeika, J., Rawson, D.M., Cionna, C., Tosti, L. and Carnevali, O., 2005. Membrane integrity and cathepsin activities of zebrafish (Danio rerio) oocytes after freezing to -196C using controlled slow cooling. Cryobiology, 51, 385-386.
  • Adams, S.L., Zhang, T. and Rawson, D.M., 2005. The effect of external medium composition on membrane water permeability of zebrafish (Danio rerio) embryos. Theriogenology, 64 (7), 1591-1602.
  • 118. Lubzens, E., Zhang, T. et al., 2005. Cryopreservation of fish oocytes: Achievements and prospects. Proceddings of Larvi' 05- Fish &Shellfish Larviculture Symposium, 296-297.
  • Zhang, T., Wex, H., Rawson, D.M. and Thompson, R.S., 2005. Rapid ecotoxicological testing using transformed BF-2 cells incorporating a luminescent reporter gene. Toxicol In Vitro, 19 (6), 797-803.
  • Zhang, T., Isayeva, A., Adams, S.L. and Rawson, D.M., 2005. Studies on membrane permeability of zebrafish (Danio rerio) oocytes in the presence of different cryoprotectants. Cryobiology, 50 (3), 285-293.
  • Kopeika, J., Zhang, T., Rawson, D.M. and Elgar, G., 2005. Effect of cryopreservation on mitochondrial DNA of zebrafish (Danio rerio) blastomere cells. Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 570 (1), 49-61.
  • Adams, S.L., Zhang, T. and Rawson, D.M., 2004. The effect of external medium composition on membrane water permeability of zebrafish (Danio rerio) embryos. Cryobiology, 49, 347-348.
  • Isayeva, A., Zhang, T. and Rawson, D.M., 2004. Studies on membrane permeability of zebrafish (Danio rerio) oocytes in the presence of different cryoprotectants. Cryobiology, 49, 346-347.
  • Plachinta, M., Zhang, T. and Rawson, D.M., 2004. Preliminary studies on cryopreservation of zebrafish (Danio rerio) vitellogenic oocytes using controlled slow cooling. Cryobiology, 49, 347.
  • Zhang, T., Bao, Q.-Y., Wang, Y. and Rawson, D.M., 2004. Development of a new rapid measurement technique for fish embryo membrane permeability studies using impedance spectroscopy. Cryobiology, 49, 318.
  • Kopeika, J., Zhang, T. and Rawson, D.M., 2004. Cryoinjury to the genome. Any consequences? Cryobiology, 49, 322.
  • Daniel, M., Sharpe, A., Driver, J., Knight, A.W., Keenan, P.O., Walmsley, M.M., Robinson, A., Zhang, T. and Rawson, D., 2004. Results of a technology demonstration project to compare rapid aquatic toxicity screening tests in the analysis of industrial effluents. Journal of Environmental Monitoring, 6 (11), 855-865.
  • Plachinta, M., Zhang, T. and Rawson, D.M., 2004. Studies on cryoprotectant toxicity to zebrafish (Danio rerio) oocytes. Cryo Letters, 25 (6), 415-424.
  • Isayeva, A., Zhang, T. and Rawson, D.M., 2004. Studies on chilling sensitivity of zebrafish (Danio rerio) oocytes. Cryobiology, 49 (2), 114-122.
  • Kopeika, J., Kopeika, E., Zhang, T., Rawson, D.M. and Holt, W.V., 2004. Effect of DNA repair inhibitor (3-aminobenzamide) on genetic stability of loach (Misgurnus fossilis) embryos derived from cryopreserved sperm. Theriogenology, 61 (9), 1661-1673.
  • Zhang, T., Robinson, A. and Rawson, D.M., 2004. Biosensor applications in toxicity determination of chemical industrial effluents. Comparative Biochemistry and Physiology, 137 (3), 117.
  • Kopeika, J., Zhang, T., Rawson, D.M. and Elgar, G., 2003. Effect of cryopreservation on mitochondrial DNA of zebrafish (Danio rerio) blastomere cells. Cryobiology, 47, 259.
  • Zhang, T., Plachinta, M., Isayeva, A. and Rawson, D.M., 2003. Studies on zebrafish (Danio rerio) oocytes sensitivity to chilling and cryoprotectant toxicity. Cryobiology, 47, 269-270.
  • Kopeika, J., Kopeika, E., Zhang, T. and Rawson, D.M., 2003. Studies on the toxicity of dimethyl sulfoxide, ethylene glycol, methanol and glycerol to loach (Misgurnus fossilis) sperm and the effect on subsequent embryo development. Cryo-Letters, 24 (6), 365-374.
  • Kopeika, J., Kopeika, E., Zhang, T., Rawson, D.M. and Holt, W.V., 2003. Effect of DNA repair inhibitor (3-Aminobenzamide) on genetic stability of loach (Misgurnus fossilis) embryos derived from cryopreserved sperm. Theriogenology, 59 (7), 1545-1556.
  • Kopeika, J., Kopeika, E., Zhang, T., Rawson, D.M. and Holt, W.V., 2003. Effect of DNA repair inhibitor (3-Aminobenzamide) on genetic stability of loach (Misgurnus fossilis) embryos derived from cryopreserved sperm. Theriogenology, 59 (7), 1545-1556.
  • Zhang, T., Liu, X.-H. and Rawson, D.M., 2003. Effects of methanol and developmental arrest on chilling injury in zebrafish (Danio rerio) embryos. Theriogenology, 59 (7), 1545-1556.
  • Kopeika, J., Kopeika, E., Zhang, T., Rawson, D.M. and Holt, W.V., 2003. Detrimental effects of cryopreservation of loach (Misgurnus fossilis) sperm on subsequent embryo development are reversed by incubating fertilised eggs in caffeine. Cryobiology, 46 (1), 43-52.
  • Kopeika, J., Kopeika, E., Zhang, T., Rawson, D.M. and Holt, W.V., 2002. Detrimental effect of loach (Misgurnus fossilis) sperm cryopreservation on subsequent embryo development are reversed by incubating fertilised eggs in caffeine. Cryobiology, 46 (1), 43-52.
  • Zhang, T. and Rawson, D.M., 2002. Studies on cryopreservation of luc gene transfected bluegill sunfish fibroblast cell line. Cryo Letters, 23 (3), 191-196.
  • Kopeika, J., Zhang, T., Kopeika, E., Holt, W.V. and Rawson, D.M., 2001. Indirect evidence of genetic effect of cryopreservation on the sperm of loach (Missgurnus fossilis). Cryobiology, 43 (4), 388.
  • Liu, X.H., Zhang, T. and Rawson, D.M., 2001. Differential scanning calorimetry studies of intraembryonic freezing and cryoprotectant penetration in zebrafish (Danio rerio) embryos. J Exp Zool, 290 (3), 299-310.
  • Liu, X.H., Zhang, T. and Rawson, D.M., 2001. Effect of cooling rate and partial removal of yolk on the chilling injury in zebrafish (Danio rerio) embryos. Theriogenology, 55 (8), 1719-1731.
  • Zhang, T., Bentley, A. and Rawson, D.M., 2001. Cryopreservation of luc gene transfected bluegill sunfish fibroblast cell line. CryoLetters, 22 (2), 81-82.
  • Liu, X.-H., Zhang, T. and Rawson, D.M., 2000. Differential scanning calorimetry study on the characterisation of intraembryonic freezing and cryoprotectant penetration in zebrafish (Danio rerio) embryos. Cryobiology, 41 (4), 355-356.
  • Rawson, D.M., Zhang, T., Kalicharan, D. and Jongebloed, W.L., 2000. Field emission scanning electron microscopy and transmission electron microscopy studies of the chorion, plasma membrane and syncytial layers of the gastrula-stage embryo of the zebrafish Brachydanio rerio: A consideration of the structural and functional relationships with respect to cryoprotectant penetration. Aquaculture Research, 31 (3), 325-336.
  • Rawson, D.M., Zhang, T., Kalicharan, D. and Jongebloed, W.L., 2000. FE-SEM and TEM studies of the chorion, plasma membrane and syncytial layers of the gastrula stage embryo of the zebrafish (Brachydanio rerio): a consideration of the structural and functional relationship with respect to cryoprotectant penetration. Aquaculture Research, 31 (3), 325-336.
  • Liu, X.-H., Zhang, T. and Rawson, D.M., 1999. Effects of cooling rates and anoxia on the chilling sensitivity of zebrafish (Danio rerio) embryos. Cryobiology, 39 (4), 318-319.
  • Liu, X.H., Zhang, T. and Rawson, D.M., 1999. The effect of partial removal of yolk on the chilling sensitivity of zebrafish (Danio rerio) embryos. Cryobiology, 39 (3), 236-242.
  • Kalicharan, D., Jongebloed, W.L., Rawson, D.M. and Zhang, T., 1998. Variations in fixation techniques for field emission SEM and TEM of zebrafish (Branchydanio rerio) embryo inner and outer membranes. Journal of Electron Microscopy, 47 (6), 645-658.
  • Liu, X.H., Zhang, T. and Rawson, D.M., 1998. Feasibility of vitrification of zebrafish (Danio rerio) embryos using methanol. Cryo-Letters, 19 (5), 309-318.
  • Zhang, T. and Rawson, D.M., 1998. Permeability of dechorionated one-cell and six-somite stage zebrafish (Brachydanio rerio) embryos to water and methanol. Cryobiology, 37 (1), 13-21.
  • Stokroos, D., Kalicharan, D., Rawson, D.M. and Zhang, T., 1998. Non coating and CRYO-FE-SEM of zebrafish (Brachydanio rerio) embryo blastodermal cells and syncytial layer. Proceedings of Dutch Society for Microscopy’98, Rynsbury.
  • Zhang, T., Rawson, D.M. and Jongebloed, W.L., 1998. Further studies on zebrafish (Brachydanio rerio) embryo membranes: permeability and ultra-structure. CryoLetters, 3, 65.
  • Liu, X.-H., Zhang, T. and Rawson, D.M., 1997. A preliminary study on vitrification of intact zebrafish (Brachydanio rerio) embryos using methanol. Cryobiology, 35 (4), 372.
  • Jongebloed, W.L., Kalicharan, D., Zhang, T. and Rawson, D.M., 1997. The zebrafish (Brachydanio rerio) egg/embryo inner and outer membrane: A FE-SEM and TEM investigation. Part 2: Fixation regimes. Proceedings of Dutch Society for Microscopy’97.
  • Rawson, D.M., Zhang, T. and Jongebloed, W.L., 1997. Structure-permeability relationships of the chorion and vitelline membranes of zebrafish (Brachydanio rerio) embryos. CryoLetters, 18 (2), 67.
  • Zhang, T. and Rawson, D.M., 1996. Permeability of the vitelline membrane of zebrafish (Brachydanio rerio) embryos to methanol and propane-1,2-diol. Cryo-Letters, 17 (5), 273-280.
  • Zhang, T., Maisse, G. and Rawson, D.M., 1996. Preliminary studies on the cryopreservation of red blood cells of rainbow trout (Oncorhynchus mykiss) and European catfish (Silurus glanis L.). Cryo-Letters, 17 (5), 303-308.
  • Zhang, T., Maisse, G. and Rawson, D.M., 1996. Preliminary studies on the cryopreservation of red blood cells of rainbow trout (Oncorhynchus mykiss) and European catfish (Silurus glanis L.). CryoLetters, 17 (6), 303-308.
  • Zhang, T. and Rawson, D.M., 1996. Permeability of the vitelline membrane of zebrafish (Brachydanio rerio) embryos to methanol and propane-1,2-diol. CryoLetters, 17 (6), 273-280.
  • Zhang, T. and Rawson, D.M., 1996. Feasibility studies on vitrification of intact zebrafish (Brachydanio rerio) embryos. Cryobiology, 33 (1), 1-13.
  • Zhang, T. and Rawson, D.M., 1995. Preliminary studies on vitelline membrane permeability of zebrafish (Brachydanio rerio) embryos. CryoLetters, 16, 319.
  • Zhang, T. and Rawson, D.M., 1995. Studies on chilling sensitivity of zebrafish (Brachydanio rerio) embryos. Cryobiology, 32 (3), 239-246.
  • Ellis, J.B., Shutes, R.B., Revitt, D.M. and Zhang, T.T., 1994. Use of macrophytes for pollution treatment in urban wetlands. Resources, Conservation and Recycling, 11 (1-4), 1-12.
  • Zhang, T. and Rawson, D.M., 1993. Cryoprotectant toxicity and permeability studies on zebrafish (Brachydanio rerio) embryos. Cryobiology, 6, 640.
  • Zhang, T., 1993. The use of zebrafish (Brachydanio rerio) for toxicity testing. Local Economy, 3, 54-57.
  • Shutes, R.B.E., Ellis, J.B., Revitt, D.M. and Zhang, T., 1993. The use of Typha latifolia for heavy metal pollution control in urban wetlands. Proceedings of International Symposium on Constructed Wetlands for Water Quality Improvement, 407-414.
  • Zhang, T., Rawson, D.M. and Morris, G.J., 1993. Cryopreservation of pre-hatch embryos of zebrafish (Brachydanio rerio). Aquatic Living Resources, 6 (2), 145-153.
  • Zhang, T. and Shutes, B., 1992. An uncommon taste for heavy metals. Industrial Waste Management, 10, 13.
  • Zhang, T., Ellis, J.B., Revitt, D.M., Shutes, R.B.E. and Xie, X.Y., 1990. The use of macrophytes for water pollution control in China and UK. IAWPRC Newsletter, 3, 25-31.
  • Zhang, T., Ellis, J.B., Revitt, D.M. and Shutes, R.B.E., 1990. Metal uptake and associated pollution control by Typha latifolia in urban wetlands. Proceedings of International Conference on the Use of Constructed Wetlands in Water Pollution Control, 451-459.
  • Li, G. and Zhang, T., 1986. Investigation of Gangutai conservation arae. Proceedings of National Conservation Symposium.
  • Li, G. and Zhang, T., 1986. Investigation of Gangutai conservation area. Proceedings of National Conservation Symposium (China), 67-75.
  • Zhang, T. and Wang, Y., 1985. The significance of Liaoning forest resources protection. Liaoning Economy (China), 3, 29-36.
  • Li, G. and Zhang, T., 1984. Aspects of environmental pollution of Liaoning western dry area. Proceedings of Agricultural Ecology Society Symposium (China), 89-101.
  • Xie, X., Zhang, Y.R. and Zhang, T., 1984. Sewage purification by water hyacinth (Eichhornia crassipes). Environmental Science (China), 5, 45-56.
  • Li, G. and Zhang, T., 1983. Effects of water pollution on Liaoning aquatic ecosystem. Proceedings of National Ecological Society Symposium (China), 75-92.

Chapters

  • Streit Jr, D.P., Godoy, L., Ribeiro, R.P., Fornari, D.C., Digmayer, M. and Zhang, T., 2014. Cryopreservation of Embryos and Oocytes of South American Fish Species. In: Yamashiro, H., ed. Recent Advances in Cryopreservation. InTech.
  • Zhang, T. and Lubzens, E., 2008. Cryopreservation of fish oocytes. Methods in Reproductive Aquaculture: Marine and Freshwater Species. CRC Press.
  • Kopeika, J., Kopeika, E., Zhang, T. and Rawson, D.M., 2008. Cryopreservation of sperm of loach (Misgurnus fossilis). Methods in Reproductive Aquaculture: Marine and Freshwater Species. CRC Press.
  • Kopeika, E., Kopeika, J. and Zhang, T., 2007. Cryopreservation of fish sperm. 203-217.
  • Zhang, T., Rawson, D.M., Pekarsky, I., Blais, I. and Lubzens, E., 2007. Low temperature preservation of fish gonad cells and oocytes. 'The Fish Oocyte: From Basic Studies to Biotechnological Applications. Kluwer Academic Publishers B.V.
  • Zhang, T., 2004. Cryopreservation of gametes and embryos of aquatic species. In: Fuller, B., Lane, N. and Benson, E., eds. 'Life in the Frozen State. CRC Press.
  • Zhang, T., 2001. Cryopreservation of fish eggs and embryos. In: Watson, P.F. and Holt, W.V., eds. Wildlife Conservation for the Future. Taylor and Francis.

Conferences

  • Anil, S., Rawson, D.M. and Zhang, T., 2016. In-vitro culture protocol development for early stage zebrafish ovarian follicles. In: Cryo2016 53rd Annual Meeting of the Society for Cryobiology. 1-4 July 2016 Otawa, Canada.
  • Zhang, T., 2015. Cryopreservation in Aquatic Species and Applications in Aquaculture and Conservation. In: International Workshop on Reproduction and Conservation in Fish 1-5 August 2015 Porto Alegre, Brazil.
  • Zhang, T., 2014. Latest development on cryopreservation of reproductive materals of fish gamates. In: Workshop on Cryopreservation of Fish Gametes and Embryos 1 December 2014-5 December 2016 Manaus, Brazil.
  • Zhang, T., 2014. Cryopreservation of aquatic species. In: 50th Society for Low Temperature Biology Annual Conference 1-4 September 2014 London.
  • Zhang, T., 2013. Cryopreservation of fishgGametes and applications in aquaculture, conservation and biomedicine. In: Workshop on the Biology of Fish Gametes 17-20 September 2013 Algarve, Portugal.
  • Ahmed, R., Spikings, E., Thompsett, A., Zhao, S. and Zhang, T., 2013. Investigation of the effects of different cryopreservation parameters on the genome of zebrafish (Danio rerio) embryos. In: Cryo2013 50rd Annual Meeting of the Society for Cryobiology July 2013 1-4 July 2013 Bethesda, Maryland, USA.
  • Anil, S., Rawson, D.M. and Zhang, T., 2013. Development of cryopreservation protocol for zebrafish ovarian fragments using controlled slow cooling. In: Cryo2013 50rd Annual Meeting of the Society for Cryobiology 1-4 July 2013 Bethesda, Maryland, USA.
  • Salavati, M., Ghafari, F., Zhang, T. and Fouladi-Nashta, A., 2012. Effects of oxygen concentration on in vitro maturation of canine oocytes. 532.
  • Ghaffarilaleh, V., Marei, W.F., Ghafari, F., Zhang, T. and Fouladi-Nashta, A., 2012. Effects of different sizes of HA on development of sheep embryos and blastocyst survival after vitrification. In: Society for Reproduction and Fertility Conference. 1-4 July 2012 Edinburgh, UK.
  • Anil, S., Zampolla, T. and Zhang, T., 2012. Development of molecular markers for stage I and stage II zebrafish ovarian follicles after in vitro culture. In: Cryo2012 49th Annual Meeting of the Society for Cryobiology. 1-4 June 2012 Rosario, Argentina.
  • Anil, S., Zampolla, T. and Zhang, T., 2012. Development of molecular markers for stage I and stage II zebrafish ovarian follicles after in vitro culture. In: Cryo2012 49th Annual Meeting of the Society for Cryobiology. 1-4 June 2012 Rosario, Argentina.
  • Godoy, L., Zampolla, T., Streit Jr, D., Bos-Mikich, A. and Zhang, T., 2012. Vitrification of zebrafish (Danio rerio) ovarian follicles. In: Cryo2012 49th Annual Meeting of the Society for Cryobiology. 1-4 June 2012 Rosario, Argentina.
  • Anil, S., Zampolla, T. and Zhang, T., 2012. Development of molecular markers for stage I and stage II zebrafish ovarian follicles after in vitro culture. In: Cryo2012 49th Annual Meeting of the Society for Cryobiology 1-4 June 2012 Rosario Argentina.
  • Zhou, S., Yan, H., Zhang, T. and Beattie, J., 2012. An investigation into the optimum conditions for separating low abundance and abundant proteins in rat plasma using a seppro column. In: 2nd International Conference on Proteomic 2012 1-4 January 2012 Unknown.
  • Zhang, T., 2011. Cryopreservation of fish gamates and its applications. In: 4th International Conference on Environmental Science 27-28 October 2011 Incheon, South Korea.
  • Zhang, T., 2011. Cryopreservation of fish gametes and its applications. In: Society of Cryobiology Annual Conference Cryo2011 24-29 July 2011 Oregon, USA.
  • Desai, K., Spikings, E. and Zhang, T., 2011. Studies on the role of hsp47 and hsp90 in zebrafish (Danio rerio) embryos under chilled conditions. In: Cryo2011 48th Annual Meeting of the Society for Cryobiology. 1-4 July 2011 Oregon, USA.
  • Anil, S., Zampolla, T. and Zhang, T., 2011. Development of in vitro culture method for zebrafish ovarian tissue fragments. In: Cryo2011 48th Annual Meeting of the Society for Cryobiology. 1-4 July 2011 Oregon, USA.
  • Desai, K., Spikings, E. and Zhang, T., 2011. Effect of chilling on sox2, sox3 and sox9 gene expression in zebrafish (Danio rerio) embryos. In: Society of Low Temperature Biology Symposium 2011 ‘Regenerative Medichine and Cryobiology’. 1-4 March 2011 London UK.
  • Zhang, T., 2011. Latest development of cryobiology research at LIRANS. In: 7. Workshop on ‘Latest Development on Cryopreservation of Fish Gametes and embryos’, Yellow Sea Fisheries Research Institute (YSFRI) 12 January 2011 Qingdao, China.
  • Zhang, T., 2010. Cryopreservation of fish gametes – current state of the art. In: 8. Workshop on Application of Cryopreservation of Fish Gametes in Aquaculture 23 November 2010 State University of Maringa, Brazil.
  • Zhang, T., 2010. Cryopreservation of fish gametes and it’s applications in aquaculture and conservation. In: 9. Workshop on Cryopreservation of Gametes and Embryos of Aquatic Species 11 November 2010 University of Rio Grande do Sul, Brazil.
  • Zampolla, T., Spikings, E., Zhang, T. and Rawson, D.M., 2010. Investigation of methanol impact on mitochondrial transport and cytoskeleton proteins in stage III zebrafish (Danio rerio) ovarian follicles. In: Cryo2010 47th Annual Meeting of the Society for Cryobiology. 1-5 July 2010 Bristol, UK.
  • Zampolla, T., Zhang, T. and Rawson, D.M., 2010. Cryoconservation and species diversity. In: The Fisheries Society of the British Isles Annual International Symposium ‘Fish and Climate Change’. 1-4 July 2010 Belfast, UK.
  • Spikings, E., Vishnolia, K., Rawson, D.M. and Zhang, T., 2010. Effect of methanol and chilling on expression of mitochondrial DNA replication factors and mitochondrial DNA replication in zebrafish embryos. In: Cryo2010 47th Annual Meeting of the Society for Cryobiology. 1-4 July 2010 Bristol, UK.
  • Anil, S., Ghafari, F., Zampolla, T., Rawson, D.M. and Zhang, T., 2010. Studies on cryoprotectant toxicity to zebrafish (Danio rerio) ovarian tissue fragments. In: Cryo2010 47th Annual Meeting of the Society for Cryobiology. 1-4 July 2010 Bristol, UK.
  • Desai, K., Spikings, E., Rawson, D.M. and Zhang, T., 2010. Studies on impact of chilling on sox gene expression in zebrafish (Danio rerio) embryos. In: Cryo2010 47th Annual Meeting of the Society for Cryobiology. 1-4 July 2010 Bristol, UK.
  • Rawson, D.M., Zhang, T. and Zampolla, T., 2010. Cryo-Banking of Cells and Tissues from Freshwater and Marine Fish in the UK Waters. In: Diversity in Biobanking: Embracing Differences, Harnessing Commonalities. Annual Meeting of International Society of Biological and Envarionmental Repositories. 1-5 May 2010 Rotterdam, Netherlands.
  • Spikings, E., Zampolla, T., Wang, Y., Rawson, D.W. and Zhang, T., 2010. Methanol disrupts mitochondrial organisation in zebrafish ovarian follicles. In: Controversies in Cryopreservation of Stem Cells, Reproductive Cells, Tissues and Organs. 1-5 April 2010 Valencia, Spain.
  • Spikings, E., Zampolla, T., Wang, Y., Rawson, D.W. and Zhang, T., 2010. Effect of cryoprotectant and chilling on oxidative stress in stage I zebrafish (Danio rerio) ovarian follicles. In: Controversies in Cryopreservation of Stem Cells, Reproductive Cells, Tissues and Organs. 1-4 April 2010 Valencia, Spain.
  • Zhang, T., Zampolla, T., Srirangarajah, S., Ramesh, S. and Rawson, D.M., 2010. Effect of cryoprotectants exposure and cryopreservation on zebrafish (Danio rerio) ovarian tissues. In: Controversies in Cryopreservation of Stem Cells, Reproductive Cells, Tissues and Organs. 1 April 2010-4 December 2016 Valencia, Spain.
  • Spikings, E., Zhang, T. and Rawson, D.M., 2009. Effect of methanol on expression of mitochondrial DNA replication factors and mitochondrial DNA replication in zebrafish embryos. In: Society for Low Temperature Biology Scientific Meeting 1-4 September 2009 Hannover, Germany.
  • Zampolla, T., Spikings, E., Zhang, T. and Rawson, D.W., 2009. Effect of methanol and DMSO exposure on mitochondrial activity and distribution in stage III ovarian follicles of zebrafish (Danio rerio). In: Cryo2009 46th Annual Meeting of the Society for Cryobiology. 1-5 July 2009 Sappora, Japan.
  • Ghafari, F., Spikings, E., Rawson, D.M. and Zhang, T., 2009. Effect of cryoprotectant treatment and chilling on oxidative stress in zebrafish (Danio rerio) early ovarian follicles. In: Cryo2009 46th Annual Meeting of the Society for Cryobiology. 1-5 July 2009 Sappora, Japan.
  • Chanzaropoulos, T., Nathanailides, C. and Zhang, T., 2008. Computer assisted speam motility analysis (CASA) of goldfish after cryopreservation. In: 4th International Congress on Aquaculture, Fisheries Technology and Environmental Management 1-5 November 2008 Athens, Greece.
  • Zhang, T., Tsai, S. and Rawson, D.M., 2008. Latest Development on Cryopreservation of Zebrafish (Danio rerio) Oocytes. In: The 10th World Conference on Animal Production. 1-5 November 2008 Cape Town, South Africa.
  • Ding, Y., Zhang, T. and Rawson, D.M., 2008. Mediated amperometric whole-cell biosensors for rapid in situ screening of heavy metal contaminated soil. In: 3rd International Conference on Soil Pollution and Remediation 1-5 October 2008 Nanjing, China.
  • Lin, C., Spikings, E., Zhang, T. and Rawson, D.M., 2008. Evaluation of potential housekeeping genes for use in expression profiling the effects of chilling and freezing in zebrafish embryos and blastomeres. In: Society for Low Temerature Biology Scientific Meeting 1-5 September 2008 Copenhagen, Denmark.
  • Spikings, E., Zhang, T. and Rawson, D.M., 2008. The Effect of Chilling on Replication of Mitochondrial DNA in Zebrafish Embryos. In: Society for Low Temerature Biology Scientific Meeting 1-5 September 2008 Copenhagen, Denmark.
  • Zampolla, T., Spikings, E., Zhang, T. and Rawson, D.M., 2008. Effect of cryoprotectants exposure on mitochondrial activity and mtDNA copy number. In: Society for Low Temerature Biology Scientific Meeting 1-5 September 2008 Copenhagen, Denmark.
  • Tsai, S., Rawson, D.M. and Zhang, T., 2008. Development of cryopreservation protocols for early stage zebrafish (Danio rerio) ovarian follicles using controlled slow cooling. In: Society for Low Temerature Biology Scientific Meeting 1-5 September 2008 Copenhagen, Denmark.
  • Rawson, D.M. and Zhang, T., 2008. Cryopreservation of somatic cells, gametes and embryos of fish. In: BioCryoBanks meeting 17-20 June 2008 Trier, Germany.
  • Spikings, E., Lin, C.-H., Zhang, T. and Rawson, D.M., 2008. The effect of chilling on mitochondrial DNA replication in zebrafish embryos. In: 8th International Meeting on Zebrafish Development & Genetics. 1-5 June 2008 Madison, USA.
  • Lin, C.-S., Zhang, T. and Rawson, D.M., 2007. Cryopreservation and gene expression in zebrafish blastomeres. In: Society for Low Temperature Biology Annual Scientific Meeting. 1-5 September 2007 Derby.
  • Tsai, S., Rawson, D.M. and Zhang, T., 2007. Studies on cryoprotectant toxicity of early stage zebrafish (Danio rerio). In: Society for Low Temperature Biology Annual Scientific Meeting. 1-5 September 2007 Derby, UK.
  • Zhang, T. and Rawson, D.M., 2007. Cryo-conservation in fish: the potentials and challenges. In: The 44th Annual Meeting of the Society for Crybiology 29 July-1 August 2007 Lake Louise, Canada.
  • Zhang, T., 2007. Cryo-conservation in fish: the potentials and challenges. In: The 44th Annual Meeting of the Society for Cryobiology 29 July-1 August 2007 Lake Louise, Canada.
  • Rawson, D.M. and Zhang, T., 2007. The Frozen Ark: Cryo-banking of genetic and cellular material of endangered species. In: The 44th Annual Meeting of the Society for Crybiology 29 July-1 August 2007 Lake Louise, Canada.
  • Lin, C.-S., Zhang, T., Elgar, G. and Rawson, D.M., 2007. Initial studies on cryopreservation and gene expression in isolated blastomeres of zebrafish (Danio rerio). In: The 44th Annual Meeting of the Society for Crybiology 29 July-1 August 2007 Lake Louise, Canada.
  • Tsai, S., Rawson, D.M. and Zhang, T., 2007. Studies on chilling sensitivity of early stage zebrafish (Danion rerio) oocytes. In: The 44th Annual Meeting of the Society for Crybiology 29 July-1 August 2007 Lake Louise, Canada.
  • Babin, P.J., Andre, M., Forgue, J., Knoll-Gellida, A., Rawson, D.M. and Zhang, T., 2007. Development of oocyte viability molecular signature (OVMS) assays for zebrafish (Danio rerio) oocyte cryopreservation studies. In: 8th International Symposium on Reproductive Physiology of Fish (ISRPF 2007) 1-5 June 2007 St Malo, France.
  • Lin, C., Zhang, T., Elgar, G. and Rawson, D.M., 2007. Cryopreservation and gene expression in zebrafish blastomeres. In: The Zebrafish Genetics and Development Meeting 1-5 July 2007 Amsterdam, The Netherlands.
  • Lubzens, E., Rosenfeld, H., Meiri, I., Olsson, P.E., Cerd, J., Babin, P.J., Admon, A., Carnevali, O., Rawson, D.M. and Zhang, T., 2007. A multidisciplinary approach for investigating the prospects for cryopreservation of fish oocytes. In: IMBC 2007 8th international marine biotechnology conference 1-5 March 2007 Eliat, Isreal.
  • Guan, M., Zhang, T. and Rawson, D.M., 2006. Effects of improved controlled slow cooling protocols on the survival of zebrafish (Danio rerio) oocytes. In: Cryo2006 1-5 July 2006 Hamburg, Germany.
  • Guan, M., Zhang, T. and Rawson, D.M., 2006. Successful enzymatic separation of zebrafish (Danio rerio) oocytes using hyaluronidase. In: Cryo2006 1-5 July 2006 Hamburg, Germany.
  • Zampolla, T., Rawson, D.M. and Zhang, T., 2006. Development of new viability assessments methods for zebrafish (Danio rerio) oocytes. In: Cryo2006 1-5 July 2006 Hamburg, Germany.
  • Zhang, T., 2005. Latest development on cryopreservation of zebrafish (Danio rerio) oocytes. In: International Meeting on Oocytes Cryopreservation 1-5 September 2005 Bordeaux, France.
  • Lubzens, E., Zhang, T. et al., 2005. Cryopreservation of fish oocytes: obstacles and achievements. In: 4th Fish & Shellfish Larviculture Symposium 1-5 September 2005 Belgium.
  • Li, Y.-Z., Zhang, T., Wang, R.Y. and Rawson, D.M., 2005. Effect of ultrasound treatment on survival of zebrafish (Danio rerio) oocytes and embryos. In: 41st Annual SLTB meeting 1-5 September 2005 York, UK.
  • Rawson, D.M., Zhang, T., Wex, H. and Bhatia, R., 2005. Cell based biosensors for environmental monitoring: challenges in exploiting cell bioassays in disposable sensor configurations. In: Annual Conference of the Society for Cryobiology (Cryo2005) 1-5 July 2005 Minneapolis USA.
  • Zhang, T., Plachinta, M., Kopeika, J., Rawson, D.M., Cionna, C., Tosti, L. and Carnevali, O., 2005. Membrane integrity and cathepsin activities of zebrafish (Danio rerio) oocytes after freezing to -196C using controlled slow cooling. In: Annual Conference of the Society for Cryobiology (Cryo2005) 1-5 July 2005 Minneapolis USA.
  • Kopeika, J., Zhang, T. and Rawson, D.M., 2005. Preliminary study on modification of yolk sec of zebrafish embryos (Danio rerio) using micro-injection. In: Annual Conference of the Society for Cryobiology (Cryo2005) 1-3 July 2005 Minneeapolis, USA.
  • Plachinta, M., Zhang, T. and Rawson, D.M., 2005. Studies on the effect of certain supplements in cryoprotective medium on zebrafish (Danio rerio) oocytes quality after controlled slow cooling. In: Annual Conference of the Society for Cryobiology (Cryo2005) 1-3 July 2005 Minneapolis, USA.
  • Zhang, T., 2005. Membrane integrity and cathepsin activities of zebrafish (Danio rerio) oocytes after freezing to -196C using controlled slow cooling. In: Annual Conference of the Society for Cryobiology (Cryo2005) 1-4 July 2005 Minneapolis, USA.
  • Hannah, W., Zhang, T. and Rawson, D.M., 2005. Use of biosensor and impedance spectroscopy assays to investigate the influence of temperature on E. coli sensitivity to 3,5-dichlorophenol. In: Bioelectrochemistry 2005 1-3 June 2005 Coimbra, Portugal.
  • Forgue, J., Knoll-Gellida, A., Andre, M., Zhang, T., Rawson, D.M. and Babin, P.J., 2005. Transcriptome analysis of zebrafish oocyte and development of an optimized cryopreservation procedure. In: Annual INRA Scientific Meeting 1-3 March 2005 Tours, France.
  • Rawson, D.M. and Zhang, T., 2005. New approaches to the cryopreservation of fish oocytes and embryos. In: International Workshop on The Role of Biotechnology for the Characterisation and Conservation of Crop, Forestry, Animal and Fishery Genetic Resources 1-3 March 2005 Turin, Italy.
  • Lubzens, E., Zhang, T. et al., 2004. Towards the development of technologies for cryopreservation of fish oocytes (CRYOCYTE). In: European Aquaculture Society Conference. 1-3 October 2004 Barcelona, Spain.
  • Zhang, T., 2004. Effect of ultrasound treatment on survival of zebrafish (Danio rerio) embryos. In: International Conferences on Preservation of Genetic Resources. 1-4 October 2004 St. Petersburg, Russia.
  • Plachinta, M., Zhang, T. and Rawson, D.M., 2004. Preliminary studies on cryopreservation of zebrafish (Danio rerio) vitellogenic oocytes using controlled slow cooling. In: World Congress of Cryobiology and Cryomedicine (CRYO'2004) 1-3 July 2004 Beijing, China.
  • Zhang, T., Bao, Q.-Y., Wang, R.Y. and Rawson, D.M., 2004. Development of a new rapid measurement technique for fish embryo membrane permeability studies using impedance spectroscopy. In: World Congress of Cryobiology and Cryomedicine (CRYO'2004) 1-3 July 2004 Beijing, China.
  • Kopeika, J., Zhang, T. and Rawson, D.M., 2004. Cryoinjury to the genome and its consequences. In: World Congress of Cryobiology and Cryomedicine (CRYO'2004) 1-3 July 2004 Beijing, China.
  • Adams, S.L., Zhang, T. and Rawson, D.M., 2004. The effect of external medium composition on membrane water permeability of zebrafish (Danio rerio) embryos. In: World Congress of Cryobiology and Cryomedicine (CRYO'2004). 1-3 July 2004 Beijing, China.
  • Zhang, T. and Rawson, D.M., 2004. Studies on membrane permeability of zebrafish (Danio rerio) oocytes in the presence of different cryoprotectants. In: World Congress of Cryobiology and Cryomedicine (CRYO'2004 1-2 July 2004 Beijing, China.
  • Zhang, T. and Rawson, D.M., 2004. Towards the development of technologies for cryopreservation of fish oocytes. In: EuroOcean 2004. European Conference on Marine Science and Ocean Technology. 1-2 May 2004 Gateway, Ireland.
  • Zhang, T., 2004. Biosensor applications in toxicity determination of chemical industrial effluents. In: Royal Society of Chemistry, Society of the Chemical Industry, Society of Environmental Toxicology & Chemistry and the Society for Experimental Biology conference. 1-4 March 2004 Edinburgh, UK.
  • Rawson, D.M. and Zhang, T., 2003. The Challenges and potential of cryopreservation of fish eggs and embryos. In: International Marine Biotechnological Industry and Science Conference. 1-3 December 2003 Taiwan.
  • Isayeva, A., Zhang, T. and Rawson, D.M., 2003. Studies on toxicity of cryoprotectants to zebrafish (Danio rerio) oocytes. In: Society for Low Temperature Biology Annual Meeting. 2-3 September 2003 Potters Bar, UK.
  • Kopeika, J., Zhang, T., Rawson, D.M. and Elgar, G., 2003. Effect of cryopreservation on mitochondrial DNA of zebrafish (Danio rerio) blastomere cells. In: CryoBioMol, 40th Annual Meeting of the Society for Cryobiology in conjunction with the Society of Low Temperature Biology. 1-2 September 2003 Coimbra, Portugal.
  • Zhang, T., Plachinta, M., Isayeva, A. and Rawson, D.M., 2003. Studies on zebrafish (Danio rerio) oocytes sensitivity to chilling and cryoprotectant toxicity. In: CryoBioMol, 40th Annual Meeting of the Society for Cryobiology in conjunction with the Society of Low Temperature Biology. 1-3 September 2003 Coimbra, Portugal.
  • Plachinta, M., Zhang, T. and Rawson, D.M., 2003. Studies on chilling sensitivity to zebrafish (Danio rerio) oocytes. In: Society for Low Temperature Biology Annual Meeting. 1-2 September 2003 Potters Bar, UK.
  • Wex, H., Zhang, T. and Rawson, D.M., 2003. The influence of temperature on the sensitivity of microbes to toxicants. In: SETAC Europe 13th Annual Meeting 1-2 May 2003 Hamburg, Germany.
  • Kopeika, J., Kopeika, E., Zhang, T., Rawson, D.M. and Holt, W.V., 2002. Effect of inhibitor of the DNA repair system on the survival of embryos (Misgurnus fossilis), derived from cryopreserved sperm. In: International Institute of Refrigeration (IIR) International Conference in association with the Society of Low Temperature Biology 1 May-2 February 2002 Hradec Kralove, Czech Republic.
  • Wex, H., Zhang, T., Depledge, M.H. and Rawson, D.M., 2001. The effects of temperature on cytotoxicity in Escherichia coli. In: Society of Environmental Toxicology and Chemistry (UK) Annual Meeting 1-2 August 2001 University of East Anglia, UK.
  • Kopeika, J., Zhang, T., Kopeika, E., Holt, W.V. and Rawson, D.M., 2001. Indirect evidence of genetic effect of cryopreservation on the sperm of loach (Missgurnus fossilis). In: CRYO 2001, 38th meeting of the Society for Cryobiology in association with the Society for Low Temperature Biology 1-2 July 2001 Edinburgh, UK.
  • Kopeika, J., Kopeika, E., Zhang, T. and Rawson, D.M., 2001. Effect of caffeine on the survival of embryos (Misgurnus fossilis) derived from cryopreserved sperm. In: Society for Low Temperature Biology Annual Meeting. 1-2 April 2001 University of Luton, UK.
  • Zhang, T., 2001. Problems associated with zebrafish (Danio rerio) embryo cryopreservation. In: 15. International Workshop on Conservation of Endangered Fish Species through the Cryopreservation of Fish Embryos. 1 March 2001 Washington DC, USA.
  • Zhang, T., 2000. Factors limiting the successful cryopreservation of zebrafish (Danio rerio) embryos. In: 16. The First International Symposium on ‘Low Temperature Gene Bank for Aquaculture Rare and Endangered Fish and Other Hydrobionts Species’. 20-23 November 2000 Pushchino, Russia.
  • Zhang, T., Bentley, A. and Rawson, D.M., 2000. Cryopreservation of luc gene transfected bluegill sunfish fibroblast cell line. In: Society for Low Temperature Biology Symposium. 1-2 September 2000 Ambleside, UK.
  • Liu, X.-H., Zhang, T. and Rawson, D.M., 2000. Differential scanning calorimetry studies of intraembryonic freezing and cryoprotectant penetration in zebrafish (Danio rerio) embryos. In: CRYO 2000, 37th Annual Meeting of the Society for Cryobiology 1-2 August 2000 Boston, USA.
  • Liu, X.-H., Zhang, T. and Rawson, D.M., 1999. Effects of cooling rates and anoxia on the chilling sensitivity of zebrafish (Danio rerio) embryos. In: World Congress of Cryobiology (CRYO’99) 1-2 July 1999 Marseilles, France.
  • Liu, X.-H., Zhang, T. and Rawson, D.M., 1998. Effect of partial removal of yolk on the survival and chilling sensitivity of zebrafish (Danio rerio) embryos. In: Society for Low Temperature Biology Symposium. 1-2 September 1998 Bristol, UK.
  • Zhang, T. and Rawson, D.M., 1997. Further studies on zebrafish (Brachydanio rerio) embryo membranes: permeability and ultra-structure. In: Society for Low Temperature Biology Symposium: Cryopreservation and the Conservation of Biodiversity. 1-2 September 1997 London, UK.
  • Liu, X.-H., Zhang, T. and Rawson, D.M., 1997. A preliminary study on vitrification of intact zebrafish (Brachydanio rerio) embryos using methanol. In: CRYO 97, 34th Annual Conference of the Society for Cryobiology 1-2 June 1997 Barcelona, Spain.
  • Rawson, D.M., Zhang, T. and Jongebloed, W., 1996. Structure-permeability relationships of the chorion and vitelline membranes of zebrafish (Brachydanio rerio) embryos. In: Society for Low Temperature Biology Symposium. 1-2 September 1996 Dundee, UK.
  • Zhang, T. and Rawson, D.M., 1995. Preliminary studies on vitelline membrane permeability of zebrafish (Brachydanio rerio) embryos. In: Society for Low Temperature Biology Conference. 1-2 September 1995 Cardiff, UK.
  • Zhang, T. and Rawson, D.M., 1994. Studies on chilling sensitivity of zebrafish (Brachydanio rerio) embryos. In: Society for Low Temperature Biology Symposium - Cryopreservation of Oocytes. 1-2 November 1994 London, UK.
  • Zhang, T. and Rawson, D.M., 1993. Cryopreservation of zebrafish embryos by supercooling. In: Society for Low Temperature Biology Annual Symposium. 1-2 September 1993 Chelwood Gate, UK.
  • Zhang, T. and Rawson, D.M., 1993. Cryoprotectant toxicity and permeability studies on zebrafish (Brachydanio rerio) embryos. In: Society for Cryobiology 30th Annual Conference. 1-2 July 1993 Atlanta, USA.
  • Zhang, T., Rawson, D.M. and Morris, G.J., 1992. Cryopreservation of pre-hatch embryos of zebrafish (Brachydanio rerio). In: International Workshop on Gamete and Embryo Storage and Cryopreservation in Aquatic Organisms. 1-2 April 1992 Paris, France.

Reports

  • Zhang, T., Plachinta, M. and Rawson, D.M., 2005. Cryopreservation of zebrafish (Danio rerio) oocytes using controlled slow cooling and vitrification. EU FPV CRYOCYTE.
  • Zhang, T., Isayeva, A., Plachinta, M. and Rawson, D.M., 2004. Studies on zebrafish (Danio rerio) oocytes membrane permeability to water and cryoprotectants and their cryopreservation using controlled slow cooling. EU FPV CRYOCYTE.
  • Zhang, T. and Rawson, D.M., 2003. Technology demonstration project: rapid aquatic toxicity screening test methods. DTI/BIO-WISE, SOCSA project.
  • Zhang, T., Isayeva, A., Plachinta, M. and Rawson, D.M., 2003. Studies on zebrafish (Danio rerio) oocytes sensitivity to chilling and cryoprotectant toxicity. Research Report, EU FPV CRYOCYTE.
  • Zhang, T. and Rawson, D.M., 2001. Development of bioassays for ecotoxicological testing, based on transformed fish cell lines incorporating luminescent reporter genes. DTI/LINK cell engineering programme.
  • Zhang, T. and Li, G., 1987. Ecological evaluation of the impact of pesticides in Liaoning province. Liaoning Environmental Protection Institute, China.
  • Zhang, T., 1985. Environmental impact of Liaoning nuclear power plant. Liaoning Environmental Protection Institute, China.

Invited Lectures

  • 1. Workshop on the Biology of Fish Gametes, Algarve, Portugal, 17-20 Sept 2013.

Grants

  • Development of cryopreservation protocols for gametes and embryos of migratory Brazilian fish (Brazilian Federal Agency for the Support & Evaluation of Graduate Education, Brazil, 01 Sep 2013). Awarded
  • Cryopreservation of oocytes of endangered species by vitrification (Science and Technology Council of HE, Ministry of Education, Brazil, 01 Jan 2011). Awarded
  • PhD Studentship (HEFCE ORSAS, 01 Sep 2009). Awarded
  • DNA Damage, Repair & Cryopreservation (HEFCE Research Informed Teaching Grant, 01 Sep 2008). Awarded
  • Cryopreservation of fish sperm (Greek Ministry of Education, 01 Sep 2005). Awarded
  • Investigation of new approaches to the cryopreservation of fish embryos (The Wellcome Trust Functional Genomics Development Initiative (GR06988MA), 01 Sep 2003). Awarded
  • Towards the development of technologies for cryopreservation of fish gametes (European Commission, Quality of Life and Management of Living Resources, 01 Sep 2002). Awarded
  • Cryopreservation of fish eggs and embryos (HEFCE, 01 Sep 1996). Awarded
  • Bioassays for ecotoxicological testing, based on transformed fish cell lines (DTI/LINK Cell Engineering Programme, 01 Sep 1992). Awarded

External Responsibilities

  • China Agriculture University, Visiting Professor (2008-)
  • BBSRC, USA, Grant Referee (2007-)
  • Journal of Aquaculture R&D, Board Member
  • Cyro Letters, Associate Editor
  • Chinese Life Science Society UK, Vice President (2011-)
  • Society of Cryobiology, Member of Board of Governors (2013-)
  • Society for Low Temperature Biology, Chairman

Qualifications

  • PhD in Cryobiology (University of Bedfordshire, 1994)
  • MPhil in Environmental Biology (Middlesex University, 1990)
  • BSc (Hons) in Environmental Biology (Liaoning University (China), 1982)
The data on this page was last updated at 04:04 on October 21, 2017.