The impact of viral mutations on recognition by SARS-CoV-2 specific T cells

Authors: de Silva, T.I., Buchan, S.L. et al.

Journal: iScience

Volume: 24

Issue: 11

eISSN: 2589-0042

DOI: 10.1016/j.isci.2021.103353

Abstract:

We identify amino acid variants within dominant SARS-CoV-2 T cell epitopes by interrogating global sequence data. Several variants within nucleocapsid and ORF3a epitopes have arisen independently in multiple lineages and result in loss of recognition by epitope-specific T cells assessed by IFN-γ and cytotoxic killing assays. Complete loss of T cell responsiveness was seen due to Q213K in the A∗01:01-restricted CD8+ ORF3a epitope FTSDYYQLY207-215; due to P13L, P13S, and P13T in the B∗27:05-restricted CD8+ nucleocapsid epitope QRNAPRITF9-17; and due to T362I and P365S in the A∗03:01/A∗11:01-restricted CD8+ nucleocapsid epitope KTFPPTEPK361-369. CD8+ T cell lines unable to recognize variant epitopes have diverse T cell receptor repertoires. These data demonstrate the potential for T cell evasion and highlight the need for ongoing surveillance for variants capable of escaping T cell as well as humoral immunity.

https://eprints.bournemouth.ac.uk/36742/

Source: Scopus

The impact of viral mutations on recognition by SARS-CoV-2 specific T cells.

Authors: de Silva, T.I. et al.

Journal: iScience

Volume: 24

Issue: 11

Pages: 103353

eISSN: 2589-0042

DOI: 10.1016/j.isci.2021.103353

Abstract:

We identify amino acid variants within dominant SARS-CoV-2 T cell epitopes by interrogating global sequence data. Several variants within nucleocapsid and ORF3a epitopes have arisen independently in multiple lineages and result in loss of recognition by epitope-specific T cells assessed by IFN-γ and cytotoxic killing assays. Complete loss of T cell responsiveness was seen due to Q213K in the A∗01:01-restricted CD8+ ORF3a epitope FTSDYYQLY207-215; due to P13L, P13S, and P13T in the B∗27:05-restricted CD8+ nucleocapsid epitope QRNAPRITF9-17; and due to T362I and P365S in the A∗03:01/A∗11:01-restricted CD8+ nucleocapsid epitope KTFPPTEPK361-369. CD8+ T cell lines unable to recognize variant epitopes have diverse T cell receptor repertoires. These data demonstrate the potential for T cell evasion and highlight the need for ongoing surveillance for variants capable of escaping T cell as well as humoral immunity.

https://eprints.bournemouth.ac.uk/36742/

Source: PubMed

The impact of viral mutations on recognition by SARS-CoV-2 specific T cells

Authors: de, S.T.I. et al.

Journal: ISCIENCE

Volume: 24

Issue: 11

eISSN: 2589-0042

DOI: 10.1016/j.isci.2021.103353

https://eprints.bournemouth.ac.uk/36742/

Source: Web of Science (Lite)

The impact of viral mutations on recognition by SARS-CoV-2 specific T cells

Authors: de Silva, T.I., Buchan, S.L. et al.

Journal: iScience

Volume: 24

Issue: 11

eISSN: 2589-0042

DOI: 10.1016/j.isci.2021.103353

Abstract:

We identify amino acid variants within dominant SARS-CoV-2 T cell epitopes by interrogating global sequence data. Several variants within nucleocapsid and ORF3a epitopes have arisen independently in multiple lineages and result in loss of recognition by epitope-specific T cells assessed by IFN-γ and cytotoxic killing assays. Complete loss of T cell responsiveness was seen due to Q213K in the A∗01:01-restricted CD8+ ORF3a epitope FTSDYYQLY207-215; due to P13L, P13S, and P13T in the B∗27:05-restricted CD8+ nucleocapsid epitope QRNAPRITF9-17; and due to T362I and P365S in the A∗03:01/A∗11:01-restricted CD8+ nucleocapsid epitope KTFPPTEPK361-369. CD8+ T cell lines unable to recognize variant epitopes have diverse T cell receptor repertoires. These data demonstrate the potential for T cell evasion and highlight the need for ongoing surveillance for variants capable of escaping T cell as well as humoral immunity.

https://eprints.bournemouth.ac.uk/36742/

Source: Manual

The impact of viral mutations on recognition by SARS-CoV-2 specific T cells.

Authors: de Silva, T.I. et al.

Journal: iScience

Volume: 24

Issue: 11

Pages: 103353

eISSN: 2589-0042

ISSN: 2589-0042

DOI: 10.1016/j.isci.2021.103353

Abstract:

We identify amino acid variants within dominant SARS-CoV-2 T cell epitopes by interrogating global sequence data. Several variants within nucleocapsid and ORF3a epitopes have arisen independently in multiple lineages and result in loss of recognition by epitope-specific T cells assessed by IFN-γ and cytotoxic killing assays. Complete loss of T cell responsiveness was seen due to Q213K in the A∗01:01-restricted CD8+ ORF3a epitope FTSDYYQLY207-215; due to P13L, P13S, and P13T in the B∗27:05-restricted CD8+ nucleocapsid epitope QRNAPRITF9-17; and due to T362I and P365S in the A∗03:01/A∗11:01-restricted CD8+ nucleocapsid epitope KTFPPTEPK361-369. CD8+ T cell lines unable to recognize variant epitopes have diverse T cell receptor repertoires. These data demonstrate the potential for T cell evasion and highlight the need for ongoing surveillance for variants capable of escaping T cell as well as humoral immunity.

https://eprints.bournemouth.ac.uk/36742/

Source: Europe PubMed Central

The impact of viral mutations on recognition by SARS-CoV-2 specific T cells

Authors: de Silva, T.I. et al.

Journal: iScience

Volume: 24

Issue: 11

ISSN: 2589-0042

Abstract:

We identify amino acid variants within dominant SARS-CoV-2 T cell epitopes by interrogating global sequence data. Several variants within nucleocapsid and ORF3a epitopes have arisen independently in multiple lineages and result in loss of recognition by epitope-specific T cells assessed by IFN-γ and cytotoxic killing assays. Complete loss of T cell responsiveness was seen due to Q213K in the A∗01:01-restricted CD8+ ORF3a epitope FTSDYYQLY207-215; due to P13L, P13S, and P13T in the B∗27:05-restricted CD8+ nucleocapsid epitope QRNAPRITF9-17; and due to T362I and P365S in the A∗03:01/A∗11:01-restricted CD8+ nucleocapsid epitope KTFPPTEPK361-369. CD8+ T cell lines unable to recognize variant epitopes have diverse T cell receptor repertoires. These data demonstrate the potential for T cell evasion and highlight the need for ongoing surveillance for variants capable of escaping T cell as well as humoral immunity.

https://eprints.bournemouth.ac.uk/36742/

Source: BURO EPrints

Preferred by: Anna Mantzouratou